FlyBase Allele Report: Dmel\w[RNAi.UAS.cKa]

General Information

Symbol

Dmel\w^RNAi.UAS.cKa

Species

D. melanogaster

Name

FlyBase ID

FBal0128964

Feature type

allele

Associated gene

Dmel\w

Associated Insertion(s)

Carried in Construct

P{UAS-w-RNAi.K}

Also Known As

W RNAi

Key Links

Transgenic product class

RNAi_reagent

(Kalidas and Smith, 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Kalidas and Smith, 2002)

Mutagen

in vitro construct

(Kalidas and Smith, 2002)

Progenitor genotype

Carried in construct

P{UAS-w-RNAi.K}

Cytology

Description

UAS sequences drive expression of the second and third exons and introns and the splice acceptor of the fourth intron of the genomic w locus, fused with an inverted w cDNA fragment.

(Kalidas and Smith, 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eye color, with Scer\GAL4^GMR.PF

(Sanders and Smith, 2011, Kalidas and Smith, 2002)

abnormal eye color, with Scer\GAL4^αTub84B.PL

(Schulz et al., 2009)

abnormal memory, with Scer\GAL4^Ddc.PL

(Sitaraman et al., 2008)

visible, with Scer\GAL4^GMR.PF

(Kalidas and Smith, 2002)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Kalidas and Smith, 2002)

eye, with Scer\GAL4^αTub84B.PL

(Schulz et al., 2009)

pigment cell, with Scer\GAL4^GMR.PF

(Kalidas and Smith, 2002)

Detailed Description

Statement

Reference

Flies expressing w^{dsRNA.Scer\UAS.cKa} under the control of Scer\GAL4^GMR.PF have defective eye pigmentation - their eyes are orange.

(Sanders and Smith, 2011)

Expression of w^{dsRNA.Scer\UAS.cKa} under the control of Scer\GAL4^αTub84B.PL leads to total eye color loss.

(Schulz et al., 2009)

Flies expressing w^{dsRNA.Scer\UAS.cKa} in serotonergic and dopaminergic neurons under the control of Scer\GAL4^Ddc.PL show conditioned memory deficits compared to controls.

Expression of w^{dsRNA.Scer\UAS.cKa} in dopaminergic neurons under the control of Scer\GAL4^ple.PF does not affect memory performance.

(Sitaraman et al., 2008)

Expression of w^{dsRNA.Scer\UAS.cKa} driven by Scer\GAL4^GMR.PF eliminates eye pigmentation.

(Kalidas and Smith, 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

w^RNAi.UAS.cKa has abnormal eye color phenotype, suppressible | somatic clone by Taf11¹/Taf11¹

(Liang et al., 2015)

w^RNAi.UAS.cKa has abnormal eye color phenotype, suppressible | somatic clone by Taf11²/Taf11²

(Liang et al., 2015)

w^RNAi.UAS.cKa has abnormal eye color phenotype, suppressible | somatic clone by Taf11³/Taf11³

(Liang et al., 2015)

w^RNAi.UAS.cKa has abnormal eye color phenotype, suppressible | somatic clone by Taf11⁴/Taf11⁴

(Liang et al., 2015)

w^RNAi.UAS.cKa has abnormal eye color phenotype, suppressible | somatic clone by Taf11⁵/Taf11⁵

(Liang et al., 2015)

w^RNAi.UAS.cKa has abnormal eye color phenotype, suppressible | somatic clone by Taf11⁶/Taf11⁶

(Liang et al., 2015)

w^RNAi.UAS.cKa has abnormal eye color phenotype, suppressible | somatic clone by Taf11⁷/Taf11⁷

(Liang et al., 2015)

Scer\GAL4^GMR.PF, w^RNAi.UAS.cKa has abnormal eye color phenotype, suppressible by AGO2⁴¹⁴

(Sanders and Smith, 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, w^RNAi.UAS.cKa has abnormal eye color phenotype, non-suppressible by blanks^KG00804

(Sanders and Smith, 2011)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The eye colour of w^{dsRNA.Scer\UAS.cKa}, Scer\GAL4^GMR.PF flies reverts to red in a AGO2⁴¹⁴ background.

The eye colour of w^{dsRNA.Scer\UAS.cKa}, Scer\GAL4^GMR.PF flies remains orange in a blanks^KG00804 background.

(Sanders and Smith, 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

w^RNAi.UAS.cKa, Scer\GAL4^αTub84B.PL, w^RESC.UAS rescues w¹¹¹⁸

(Schulz et al., 2009)

Comments

Co-expression of w^{RESC.Scer\UAS} and w^{dsRNA.Scer\UAS.cKa} under the control of Scer\GAL4^αTub84B.PL restores eye color in w¹¹¹⁸ mutants. The eye color seen is identical to that seen when only w^{RESC.Scer\UAS} is expressed.

(Schulz et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

65409

Taf11⁵ P{neoFRT}40A/CyO; P{UAS-w-RNAi.K}3, P{GAL4-ninaE.GMR}3, e¹/TM6B, Tb⁺

65410

Taf11¹ P{neoFRT}40A/CyO; P{UAS-w-RNAi.K}3, P{GAL4-ninaE.GMR}3, e¹/TM6B, Tb⁺

Notes on Origin

Discoverer

Comments

Transcription from the P{UAS-w-dsRNA.K} produces w dsRNA, resulting in dsRNA interference (RNAi) of the w.

(Kalidas and Smith, 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

w^RNAi.UAS.cKa

w^{dsRNA.Scer\UAS.cKa}

w^{dsRNA.Scer\UAS}

w^{dsRNA.UAS.cKa}

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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