FlyBase Allele Report: Dmel\otk[3]

General Information

Symbol

Dmel\otk³

Species

D. melanogaster

Name

FlyBase ID

FBal0127920

Feature type

allele

Associated gene

Dmel\otk

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Winberg et al., 2001)

Mutagen

Progenitor genotype

otk^EP2017

(Winberg et al., 2001)

Cytology

Description

Imprecise excision of the P{EP} insertion in otk^EP2017 has led to a 3 kb deletion that extends downstream of the insertion. The putative start codon and the signal peptide have been removed.

(Winberg et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

axon

(Winberg et al., 2001)

eye photoreceptor cell & axon & larva | somatic clone

(Cafferty et al., 2004)

lamina & glial cell & larva | somatic clone

(Cafferty et al., 2004)

larval abdominal segmental nerve

(Winberg et al., 2001)

larval intersegmental nerve

(Winberg et al., 2001)

medulla | somatic clone

(Cafferty et al., 2004)

photoreceptor cell R1 & axon & larva | somatic clone

(Cafferty et al., 2004)

photoreceptor cell R1 & axon | somatic clone

(Cafferty et al., 2004)

photoreceptor cell R2 & axon & larva (with otk^EP2017)

(Cafferty et al., 2004)

photoreceptor cell R2 & axon & larva | somatic clone

(Cafferty et al., 2004)

photoreceptor cell R2 & axon | somatic clone

(Cafferty et al., 2004)

photoreceptor cell R3 & axon & larva (with otk^EP2017)

(Cafferty et al., 2004)

photoreceptor cell R3 & axon & larva | somatic clone

(Cafferty et al., 2004)

photoreceptor cell R3 & axon | somatic clone

(Cafferty et al., 2004)

photoreceptor cell R4 & axon & larva (with otk^EP2017)

(Cafferty et al., 2004)

photoreceptor cell R4 & axon & larva | somatic clone

(Cafferty et al., 2004)

photoreceptor cell R4 & axon | somatic clone

(Cafferty et al., 2004)

photoreceptor cell R5 & axon & larva (with otk^EP2017)

(Cafferty et al., 2004)

photoreceptor cell R5 & axon & larva | somatic clone

(Cafferty et al., 2004)

photoreceptor cell R5 & axon | somatic clone

(Cafferty et al., 2004)

photoreceptor cell R6 & axon & larva (with otk^EP2017)

(Cafferty et al., 2004)

photoreceptor cell R6 & axon & larva | somatic clone

(Cafferty et al., 2004)

photoreceptor cell R6 & axon | somatic clone

(Cafferty et al., 2004)

ventral denticle belt

(Peradziryi et al., 2011)

Detailed Description

Statement

Reference

otk³/+ larvae do not display increased dendrite crossing in the class IV dendrite arborizing neurons.

(Meltzer et al., 2016)

Mutant embryos show loss of several rows of denticles per segment, with more cells adopting a named cuticle fate.

(Peradziryi et al., 2011)

otk³ homozygous photoreceptor cells in somatic clones in the late third instar larval retina have defective axonal projections: small gaps are frequently observed in the R1-R6 terminal field; the terminal field within the medulla is disorganized, with abnormally thick bundles frequently formed; more than 32% (average 33 axons or axon bundles per hemisphere, n=16 hemispheres) of ommatidia project one or more R2-R5 axons or axon bundles aberrantly into the medulla. Despite this, overall organization of photoreceptor cell axons within the developing optic lobe to form the topographic map appears normal. The organisation of photoreceptors into ommatidia in the retina also appears normal in these clones. In adults carrying these clones, an abnormally high number of eye photoreceptor cell axons terminate in the the medulla. These include R1-R6 cell axons, which normally all terminate in the lamina: approximately 42% of mutant ommatidia project one or more R1-R6 axons aberrantly into the medulla. In contrast, R7 axon targetting is normal In late third instar larvae carrying otk³ homozygous clones, lamina glial cells appear less organized than that in wild type, but the numbers of lamina glial cells surrounding the lamina plexus are normal. In late third instar otk³/otk^EP2017 there are minor defects in axonal projections from developing eye photoreceptor cells: an average of 18 R2-R5 axons or axon bundles per hemisphere are mistargeted.

(Cafferty et al., 2004)

Mutants exhibit an axon guidance phenotype. The dorsal branch of the SNa, about a fifth of the time, does not divide in the proper location but rather extends as a single thicker branch, remaining fasciculated together in over 60% of segments. The ISNb can sometimes fail to defasciculate from the ISN, and the branches from the ISNb to individual muscles are also sometimes bypassed. The central nervous system of these animals are also affected. The outer tracts are discontinuous, with a clumped appearance where axons have stalled. Inner tracts are somewhat "unravelled".

(Winberg et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

otk³ has larval abdominal segmental nerve phenotype, enhanceable by Df(4)C3

(Winberg et al., 2001)

otk³ has larval intersegmental nerve phenotype, enhanceable by Df(4)C3

(Winberg et al., 2001)

otk³ has larval intersegmental nerve phenotype, enhanceable by Sema1a^unspecified

(Winberg et al., 2001)

otk³ has larval abdominal segmental nerve phenotype, enhanceable by Sema1a^unspecified

(Winberg et al., 2001)

Enhancer of

Statement

Reference

otk³ is an enhancer of larval intersegmental nerve phenotype of Sema1a^unspecified

(Winberg et al., 2001)

otk³ is an enhancer of larval abdominal segmental nerve phenotype of Sema1a^unspecified

(Winberg et al., 2001)

Suppressor of

Statement

Reference

otk³/otk[+] is a suppressor of motor neuron phenotype of Scer\GAL4^l(3)H94-H94, Sema1a^UAS.cYa

(Winberg et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

otk³,Df(2R)Sema-2b-C4 double mutant larvae do not display increased dendrite crossing in the class IV dendrite arborizing neurons.

(Meltzer et al., 2016)

Embryos expressing Wnt4^Scer\UAS.cGa under the control of Scer\GAL4^da.G32 in a otk³ background show loss of denticle rows similar to that see in otk³ single mutants.

Embryos expressing wg^Scer\UAS.cHa under the control of Scer\GAL4^da.G32 in a otk³ background have no denticles.

Homozygous clones in the wing do not result in a mutant phenotype.

(Peradziryi et al., 2011)

The addition of Sema-1a^unspecified/+ or Df(4)C3/+ enhances the SNa and ISNb axon guidance phenotypes seen in otk³/+ flies.

(Winberg et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

otk^EP2017/otk³ is rescued by otk^UAS.cWa/Scer\GAL4^GMR.PF

(Cafferty et al., 2004)

otk³ is rescued by otk^UAS.cWa/Scer\GAL4^elav.PLu

(Winberg et al., 2001)

Comments

Mistargetting of developing eye photoreceptor axons in otk³/otk^EP2017 late third instar larvae is largely suppressed by otk^Scer\UAS.cWa; Scer\GAL4^GMR.PF.

(Cafferty et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

92359

w^*; otk³/CyO, P{en1}wg^en11

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

otk³

(Xie et al., 2022, Meltzer et al., 2016, Peradziryi et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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