At 29^oC, expression of en::Xlae\Meis3^{1-333.Scer\UAS} under the control of Scer\GAL4^dpp.blk1 results in pupal lethality. At 24^oC, pharate adults are formed. The pharate adults have enlarged eyes and in many cases ectopic eyes are seen. The antennae are partially transformed into legs; the third segment is transformed distally to distal leg tissue. The endogenous legs are malformed and truncated, with reduced proximal segments, abnormal mid-leg and loss of the most distal structures. Outgrowth of leg-like structures from the anal region is seen in flies expressing en::Xlae\Meis3^{1-333.Scer\UAS} under the control of Scer\GAL4^dpp.blk1. The outgrowths have distal leg identity, as suggested by the presence of bracted bristles. A substantial enlargement of the presumptive anal region is seen in the genital discs.

Scer\GAL4^dpp.blk1, Xlae\Meis3^1-333.UAS has lethal | heat sensitive phenotype, suppressible by hth^UAS.cPa/Scer\GAL4^dpp.blk1