FB2024_03 , released June 25, 2024
Allele: Dmel\ago4
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General Information
Symbol
Dmel\ago4
Species
D. melanogaster
Name
FlyBase ID
FBal0126455
Feature type
allele
Associated gene
Dmel\ago
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Moberg et al., 2001)
Progenitor genotype
Cytology
Description

Amino acid replacement: A1117V.

(Moberg et al., 2001)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3L:4,248,319..4,248,319 [-]
Nucleotide change:

C4248319T

Amino acid change:

A1117V | ago-PA; A1117V | ago-PB; A1117V | ago-PC

Reported amino acid change:

A1117V

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Moberg et al., 2001)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      When ago4 mutant clones are induced in the ovary, some cysts have fewer nurse cells than wild-type, some have more, and many degenerate. In addition, strongly DAPI staining regions of nurse cell nuclei (which may respond to compact heterochromatin) are enlarged compared to wild-type.

      (Doronkin et al., 2003)

      Adult eyes mosaic for mutations in ago are composed mostly of mutant tissue, exhibiting a proliferative advantage over their wild-type twin-spots.

      (Moberg et al., 2001)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Statement
      Reference

      ago4/ago[+] is an enhancer of visible phenotype of MycUAS.cZa, Scer\GAL4GMR.PF

      (Secombe et al., 2007)
      Other
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      ago4/ago[+] is an enhancer of eye phenotype of MycUAS.cZa, Scer\GAL4GMR.PU

      (Li et al., 2013)

      ago4/ago[+] is an enhancer of eye phenotype of MycUAS.cZa, Scer\GAL4GMR.PF

      (Secombe et al., 2007)
      Other
      Statement
      Reference

      CSN5quo-1, ago4 has nurse cell phenotype

      (Doronkin et al., 2003)

      CSN5quo-1, ago4 has nurse cell & nucleus phenotype

      (Doronkin et al., 2003)

      CSN5quo-1, ago4/ago[+] has nurse cell phenotype

      (Doronkin et al., 2003)

      CSN5quo-1, ago4/ago[+] has nurse cell & nucleus phenotype

      (Doronkin et al., 2003)

      CSN5quo-1/CSN5[+], ago4 has nurse cell phenotype

      (Doronkin et al., 2003)

      CSN5quo-1/CSN5[+], ago4 has nurse cell & nucleus phenotype

      (Doronkin et al., 2003)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Unlike either single heterozygote, ovaries from CSN5quo-1; ago4 transheterozygous females exhibit ectopic cell death and increased or decreased nurse cells per egg chamber. Decreased nurse cell numbers per cyst are accompanied by an increase in nurse cell ploidy, suggesting that the decrease results from a block in cell division without a block in DNA replication. In addition, strongly DAPI staining regions of nurse cell nuclei (which may respond to compact heterochromatin) are enlarged compared to wild-type.

      (Doronkin et al., 2003)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      ago4
      (Bandura et al., 2013, Li et al., 2013, Secombe et al., 2007, Firth and Baker, 2005)
      Name Synonyms
      Secondary FlyBase IDs
        References (7)