FB2024_03 , released June 25, 2024
Allele: Dmel\NDN.UAS
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General Information
Symbol
Dmel\NDN.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0125378
Feature type
allele
Associated gene
Dmel\N
Associated Insertion(s)
Carried in Construct
P{UAS-N.DN}
Also Known As
UAS-NDN, UAS-NotchDN
Key Links
Transgenic product class
dominant_negative_variant
(Kumar and Moses, 2001)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Kumar and Moses, 2001)
Mutagen
in vitro construct
(Kumar and Moses, 2001)
Progenitor genotype
Carried in construct
P{UAS-N.DN}
Cytology
Description

UAS regulatory sequences drive expression of a dominant negative form of N.

(Kumar and Moses, 2001)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
N
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      model of  cancer
      is ameliorated by Atg1UAS.cSa
      (Lee et al., 2021)
      is ameliorated by wgJF01257
      (Lee et al., 2021)
      ameliorates  carcinoma
      modeled by Vps25N55
      (Herz et al., 2006)
      ameliorates  intestinal cancer
      modeled by ykiS111A.S168A.S250A.UAS.Tag:V5
      (Pranoto et al., 2023)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of NDN.Scer\UAS results in adults exhibiting supernumerary thoracic sensory bristles (under the control of either Scer\GAL4sca-C253 or Scer\GAL4pros.PMG) and results in third instar larval wing discs exhibiting an increased number of sensory organ precursor cells (under the control of Scer\GAL4sca-C253), as compared to controls.

      (Shukla et al., 2017)

      Expression of NDN.Scer\UAS under the control of Scer\GAL4esg-NP5130 (in combination with a Gal80[ts] transgene to restrict expression to the adult stage) results in a significant increase in the number of mitotic cells in the midgut, as compared to controls.

      (Nie et al., 2015)

      Flies expressing NDN.Scer\UAS under the control of Scer\GAL4bi.PU show distal vein thickenings.

      (Pereira et al., 2006)

      Eye-antennal discs in which NECN.Scer\UAS is expressed under the control of either Scer\GAL4ey.PB or Scer\GAL4ey.PH display small or no eye fields by mid- to late-L3 and some discs display full or partial double antennal domains. This leads to adult flies lacking eyes and sometimes displaying double antennae. The double antennae appear to originate from a duplication of the antenna primordium, rather than an eye-to-antenna transformation. Expression of NDN.Scer\UAS, under the control of Scer\GAL4ey.PB or Scer\GAL4ey.PH, in mid-late L2 eye-antennal discs results in a severe suppression of cell division, shown by a decreased number of mitotic cells.

      (Kenyon et al., 2003)

      When NDN.Scer\UAS is driven by Scer\GAL4sca-P309, a weak reduction in external structures on the adult antenna is seen, with a concomitant increase in glial cell numbers, with a concomitant increase in glial cell numbers. The diameter of fascicles in antennal segment 3 are also increased. When Scer\GAL4pros.PMG is driven by Scer\GAL4pros.PMG, no defects in external cells are seen on antennal segment 3.

      (Sen et al., 2003)

      Expression of NDN.Scer\UAS under the control of Scer\GAL4ey.PB can result in homeotic transformation of the eye to antenna. Third instar larvae show complete replacement of the eye disc by an antennal disc.

      (Kumar and Moses, 2001)

      Expression of NDN.Scer\UAS under the control of Scer\GAL4dpp.blk1 inhibits furrow reincarnation but not birth in the eye disc.

      (Kumar and Moses, 2001)

      When expression is driven by Scer\GAL4btl.PS, ectopic fusions occur between adjacent dorsal branches and terminal branching is repressed. The phenotype is similar to that caused by HScer\UAS.cGa, but is less penetrant.

      (Llimargas, 1999)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      Enhancer of
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Other
      Phenotype Manifest In
      Enhanced by
      NOT Enhanced by
      Suppressed by
      Statement
      Reference

      NDN.UAS, Scer\GAL4ey.PB has eye disc phenotype, suppressible by CycEUAS.cLa, Scer\GAL4ey.PB

      (Kenyon et al., 2003)

      NDN.UAS, Scer\GAL4ey.PH has eye disc phenotype, suppressible by CycEUAS.cLa, Scer\GAL4ey.PH

      (Kenyon et al., 2003)
      Enhancer of
      NOT Enhancer of
      Statement
      Reference

      NDN.UAS/Scer\GAL4Tub.PU is a non-enhancer of eye disc | somatic clone phenotype of Vps25N55

      (Herz et al., 2006)
      Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The co-expression of both NDN.Scer\UAS and Rbfox1dsRNA.Scer\UAS strongly enhances both the supernumerary thoracic sensory bristle phenotype in adults (when under the control of Scer\GAL4sca-C253, but not when under the control of Scer\GAL4pros.PMG) and the increased number of sensory organ precursor cells in third instar larval wing discs (when under the control of Scer\GAL4sca-C253) which result from the expression of either NDN.Scer\UAS alone or Rbfox1dsRNA.Scer\UAS alone.

      The co-expression of both NDN.Scer\UAS and Rbfox1Scer\UAS.cUa under the control of Scer\GAL4sca-C253 results in the virtual loss of thoracic sensory macrochaeta, but not microchaeta, as compared to controls.

      (Shukla et al., 2017)

      Co-expression of bunGD1392 suppresses the increased number of mitotic cells seen in the midguts of flies expressing NDN.Scer\UAS under the control of Scer\GAL4esg-NP5130.

      (Nie et al., 2015)

      Vps25N55/NDN.Scer\UAS mutants (using the MARCM technique) exhibit reduced or absent Stat92E activity. Cell proliferation is not significantly increased in Vps25N55/NDN.Scer\UAS mutants, with eye imaginal discs appearing normal in shape and size. These Vps25N55/NDN.Scer\UAS double mutant clones exhibit the same level of apoptosis as Vps25N55 single mutant clones

      (Herz et al., 2006)

      When CycEScer\UAS.cLa is expressed under the control of either Scer\GAL4ey.PB or Scer\GAL4ey.PH in NDN.Scer\UAS eye discs, rescue of the NDN.Scer\UAS mitotic phenotype is seen; eye discs are larger and contain more proliferating cells.

      (Kenyon et al., 2003)

      Furrow initiation is blocked in eye discs coexpressing phlAct.Scer\UAS and NDN.Scer\UAS under the control of Scer\GAL4dpp.blk1.

      (Kumar and Moses, 2001)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      N(DN)
      (Nystul and Spradling, 2010)
      NDN.Scer\UAS
      NDN.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (22)