The median lifespan for prosV27 animals (the age after egg laying at which 50% of homozygotes have died) is 2.66 days at 25oC.
Homozygous third instar larvae show reduced locomotor activity compared to controls.
Homozygous second instar larvae are indifferent to 0.1M sucrose in a taste response assay, in contrast to wild-type larvae which are significantly attracted by sucrose. They are repulsed by 0.3M NaCl (as are wild-type larvae).
The main branch of the neuromuscular junction of muscles 6 and 7 of the second abdominal segment has fewer secondary ramifications in mutant larvae compared to that of wild-type larvae.
The courtship index of heterozygous males towards decapitated Canton-S males is significantly higher than that of wild-type males towards decapitated Canton-S males. The courtship index of heterozygous males towards decapitated Canton-S males is not significantly different from that of heterozygous prosV1 males towards decapitated Canton-S males. Lethality of homozygotes occurs throughout most developmental stages.