FlyBase Allele Report: Dmel\rl[UAS.cKa]

General Information

Symbol

Dmel\rl^UAS.cKa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Kumar

FlyBase ID

FBal0124962

Feature type

allele

Associated gene

Dmel\rl

Associated Insertion(s)

Carried in Construct

P{UAS-rl.K}

Also Known As

UAS-rl, UAS::rolled

Key Links

Transgenic product class

wild_type

(Kumar and Moses, 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Kumar and Moses, 2001)

Mutagen

in vitro construct

(Kumar and Moses, 2001)

Progenitor genotype

Carried in construct

P{UAS-rl.K}

Cytology

Description

UAS regulatory sequences drive expression of rl.

(Kumar and Moses, 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^sd-SG29.1

(Mouchel-Vielh et al., 2008)

visible | adult stage, with Scer\GAL4^sd.PU

(Mouchel-Vielh et al., 2011)

Phenotype Manifest In

wing vein | ectopic, with Scer\GAL4^sd.PU

(Mouchel-Vielh et al., 2011)

wing vein | ectopic, with Scer\GAL4^sd-SG29.1

(Mouchel-Vielh et al., 2008)

wing vein | ectopic | increased number, with Scer\GAL4^Bx-MS1096

(Maeng et al., 2012)

Detailed Description

Statement

Reference

Expression of rl^Scer\UAS.cKa using Scer\GAL4^Bx-MS1096 generates extra and ectopic veins in various wing regions.

(Maeng et al., 2012)

Expression of rl^UAS.cKa under the control of Scer\GAL4^sd.PU leads to ectopic wing veins.

(Mouchel-Vielh et al., 2011)

100% of flies expressing rl^Scer\UAS.cKa under the control of Scer\GAL4^sd-SG29.1 have an ectopic wing vein phenotype.

(Mouchel-Vielh et al., 2008)

Expression of rl^Scer\UAS.cKa under the control of Scer\GAL4^ey.PB does not result in homeotic transformation of the eye to antenna.

(Kumar and Moses, 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^sd.PU, rl^UAS.cKa has visible | adult stage phenotype, enhanceable by corto^07128b/corto[+]

(Mouchel-Vielh et al., 2011)

NOT Enhancer of

Statement

Reference

rl^UAS.cKa, Scer\GAL4^GMR.PU is a non-enhancer of visible | adult stage phenotype of Hsap\KCNC3^R423H.UAS, Scer\GAL4^GMR.PU

(Khare et al., 2017)

NOT Suppressor of

Statement

Reference

rl^UAS.cKa, Scer\GAL4^GMR.PU is a non-suppressor of visible | adult stage phenotype of Hsap\KCNC3^R423H.UAS, Scer\GAL4^GMR.PU

(Khare et al., 2017)

Other

Statement

Reference

Lamtor3^RNAi.UASp, Scer\GAL4^sd-SG29.1, rl^UAS.cKa has visible phenotype

(Mouchel-Vielh et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^Bx-MS1096, rl^UAS.cKa has wing vein | ectopic | increased number phenotype, enhanceable by Plzf^UAS.cMa, Scer\GAL4^Bx-MS1096

(Maeng et al., 2012)

Scer\GAL4^sd.PU, rl^UAS.cKa has wing vein | ectopic phenotype, enhanceable by corto^07128b/corto[+]

(Mouchel-Vielh et al., 2011)

Suppressed by

Statement

Reference

Scer\GAL4^Bx-MS1096, rl^UAS.cKa has wing vein | ectopic | increased number phenotype, suppressible by Plzf^Δ145/Plzf[+]

(Maeng et al., 2012)

Scer\GAL4^Bx-MS1096, rl^UAS.cKa has wing vein | ectopic | increased number phenotype, suppressible by Plzf^Δ11/Plzf[+]

(Maeng et al., 2012)

Enhancer of

Statement

Reference

rl^UAS.cKa, Scer\GAL4^Bx-MS1096 is an enhancer of wing vein | ectopic | increased number phenotype of Plzf^UAS.cMa, Scer\GAL4^Bx-MS1096

(Maeng et al., 2012)

NOT Enhancer of

Statement

Reference

rl^UAS.cKa, Scer\GAL4^GMR.PU is a non-enhancer of eye phenotype of Hsap\KCNC3^R423H.UAS, Scer\GAL4^GMR.PU

(Khare et al., 2017)

NOT Suppressor of

Statement

Reference

rl^UAS.cKa, Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Hsap\KCNC3^R423H.UAS, Scer\GAL4^GMR.PU

(Khare et al., 2017)

Other

Statement

Reference

Lamtor3^RNAi.UASp, Scer\GAL4^sd-SG29.1, rl^UAS.cKa has wing phenotype

(Mouchel-Vielh et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Scer\GAL4^Bx-MS1096-mediated expression of both rl^Scer\UAS.cKa and Plzf^Scer\UAS.cMa generates significantly enhanced extra and ectopic veins as compared to expression of either transgene alone. This effect is more dramatic at 18[o]C than 25[o]C.

The Scer\GAL4^Bx-MS1096, rl^Scer\UAS.cKa extra wing vein phenotype is completely suppressed in Plzf^Δ145/+ or Plzf^Δ11/+ heterozygotes.

(Maeng et al., 2012)

corto^07128b/+ enhances the ectopic wing vein phenotype induced by expression of rl^UAS.cKa under the control of Scer\GAL4^sd.PU.

(Mouchel-Vielh et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^elav-C155/rl^UAS.cKa partially rescues rl¹

(Park et al., 2017)

rl^UAS.cKa/Scer\GAL4^Mef2.PU partially rescues rl¹

(Park et al., 2017)

Comments

Scer\GAL4^elav-C155-driven expression of rl^Scer\UAS.cKa efficiently rescues the ectopic targetting of type III axons to the abdominal body-wall muscle 13 but fails to rescue the premature defasciculation of axons bundles at the boundary between muscles 13 and 12 in rl¹ mutant third instar larvae. Scer\GAL4^Mef2.PU-controlled expression significantly improves both of these innervation defects.

(Park et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

36270

w¹¹¹⁸; P{UAS-rl.K}2A

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

rl^Scer\UAS.cKa

rl^UAS.cKa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Kumar

(Kumar and Moses, 2001)

Secondary FlyBase IDs

References (8)

Report Sections

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