FlyBase Allele Report: Dmel\grim::rpr[rg.UAS]

General Information

Symbol

Dmel\grim::rpr^rg.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0123850

Feature type

allele

Associated gene

grim::rpr

Associated Insertion(s)

Carried in Construct

P{UAS-r/grim}

Also Known As

P[UAS-r/grim]

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Wing et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-r/grim}

Cytology

Description

Amino acids 1-14 of rpr are linked to amino acids 15-138 of grim. This chimera is expressed under the control of UASt sequences.

(Wing et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

grim::rpr

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eye color | heat sensitive, with Scer\GAL4^GMR.PF

(Wing et al., 2002)

lethal, with Scer\GAL4^GMR.PF

(Wing et al., 2001)

visible, with Scer\GAL4^GMR.PF

(Zhou et al., 2013, Wing et al., 2002, Wing et al., 2001)

visible | heat sensitive, with Scer\GAL4^GMR.PF

(Wing et al., 2002)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Zhou et al., 2013, Wing et al., 2002, Wing et al., 2001)

eye | heat sensitive, with Scer\GAL4^GMR.PF

(Wing et al., 2002)

ommatidium, with Scer\GAL4^GMR.PF

(Wing et al., 2002)

pigment cell, with Scer\GAL4^GMR.PF

(Wing et al., 2002)

Detailed Description

Statement

Reference

Flies expressing grim::rpr^rg.Scer\UAS under the control of Scer\GAL4^GMR.PF exhibit a moderate eye cell death phenotype that includes a reduction in size and pigmentation of the adult compound eye.

(Zhou et al., 2013)

grim::rpr^rg.Scer\UAS when driven by Scer\GAL4^GMR.PF leads to a temperature sensitive loss of eye tissue and pigmentation.

(Wing et al., 2002)

Flies expressing grim::rpr^rg.Scer\UAS under the control of Scer\GAL4^GMR.PF show a slight reduction in eye size and roughening of the eye at 17^oC. At 26^oC, a strong loss of eye tissue is seen. Reduced eye pigmentation is also seen in flies expressing grim::rpr^rg.Scer\UAS under the control of Scer\GAL4^GMR.PF.

(Wing et al., 2002)

Expression of grim::rpr^rg.Scer\UAS when driven by Scer\GAL4^Gp150-52A does not result in ectopic midline cell death. Expression of grim::rpr^rg.Scer\UAS when driven by Scer\GAL4^GMR.PF can lead to lethality, or semi-lethality with a moderate loss of eye tissue (depending on the particular insertion used).

(Wing et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has visible phenotype, enhanceable by morgue^G421C.UAS/Scer\GAL4^GMR.PF

(Zhou et al., 2013)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has visible phenotype, enhanceable by morgue^G421A.UAS/Scer\GAL4^GMR.PF

(Zhou et al., 2013)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has visible phenotype, enhanceable by morgue^G421S.UAS/Scer\GAL4^GMR.PF

(Zhou et al., 2013)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has visible phenotype, enhanceable by morgue^{UAS.N.Tag:FLAG}/Scer\GAL4^GMR.PF

(Zhou et al., 2013)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has visible phenotype, enhanceable by morgue^{UAS.C.Tag:FLAG}/Scer\GAL4^GMR.PF

(Zhou et al., 2013)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has visible phenotype, enhanceable by morgue^UAS.cZa/Scer\GAL4^GMR.PF

(Zhou et al., 2013)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has abnormal eye color | heat sensitive phenotype, enhanceable by skl^UAS.cWa/Scer\GAL4^GMR.PF

(Wing et al., 2002)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has visible phenotype, enhanceable by Scer\GAL4^GMR.PF/morgue^EP2367

(Wing et al., 2002)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has visible phenotype, enhanceable by Scer\GAL4^GMR.PF/Uba1^EP2375

(Wing et al., 2002)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has visible phenotype, enhanceable by SkpA^EP1423/Scer\GAL4^GMR.PF

(Wing et al., 2002)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has visible phenotype, non-enhanceable by morgue^ΔFB.UAS/Scer\GAL4^GMR.PF

(Zhou et al., 2013)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has visible phenotype, non-enhanceable by morgue^ΔUEV.UAS/Scer\GAL4^GMR.PF

(Zhou et al., 2013)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has visible phenotype, non-enhanceable by morgue^ΔZF.UAS/Scer\GAL4^GMR.PF

(Zhou et al., 2013)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS, skl^UAS.cWa has abnormal eye color | heat sensitive phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PF

(Wing et al., 2002)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS, skl^UAS.cWa has lethal phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PF

(Wing et al., 2002)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has visible phenotype, suppressible by Df(2L)dp-h25/+, Scer\GAL4^GMR.PF

(Wing et al., 2002)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has visible phenotype, suppressible by Df(2L)morgue¹⁹/+, Scer\GAL4^GMR.PF

(Wing et al., 2002)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has visible phenotype, suppressible by morgue[+]/morgue^EP1184/Scer\GAL4^GMR.PF

(Wing et al., 2002)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has visible phenotype, suppressible by SkpA^EP1216a/EP1216b^EP1216b, Scer\GAL4^GMR.PF

(Wing et al., 2002)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has lethal phenotype, suppressible by Dronc^C318S.GMR

(Wing et al., 2001)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has visible phenotype, suppressible by Dronc^C318S.GMR

(Wing et al., 2001)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has lethal phenotype, suppressible by Diap1^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Wing et al., 2001)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has lethal phenotype, suppressible | partially by Diap2^UAS.cWa, Scer\GAL4^GMR.PF

(Wing et al., 2001)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has visible phenotype, suppressible by Diap1^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Wing et al., 2001)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has visible phenotype, suppressible | partially by Diap2^UAS.cWa, Scer\GAL4^GMR.PF

(Wing et al., 2001)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has lethal phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PF

(Wing et al., 2001)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has visible phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PF

(Wing et al., 2001)

Other

Statement

Reference

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS, skl^UAS.cWa has lethal phenotype

(Wing et al., 2002)

Scer\GAL4^GMR.PF, faf^EP3520, grim::rpr^rg.UAS has lethal phenotype

(Wing et al., 2002)

Scer\GAL4^GMR.PF, SkpA^EP1423, grim::rpr^rg.UAS has partially lethal - majority die phenotype

(Wing et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has eye phenotype, enhanceable by morgue^G421C.UAS/Scer\GAL4^GMR.PF

(Zhou et al., 2013)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has eye phenotype, enhanceable by morgue^G421A.UAS/Scer\GAL4^GMR.PF

(Zhou et al., 2013)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has eye phenotype, enhanceable by morgue^G421S.UAS/Scer\GAL4^GMR.PF

(Zhou et al., 2013)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has eye phenotype, enhanceable by morgue^{UAS.N.Tag:FLAG}/Scer\GAL4^GMR.PF

(Zhou et al., 2013)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has eye phenotype, enhanceable by morgue^{UAS.C.Tag:FLAG}/Scer\GAL4^GMR.PF

(Zhou et al., 2013)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has eye phenotype, enhanceable by morgue^UAS.cZa/Scer\GAL4^GMR.PF

(Zhou et al., 2013)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has eye | heat sensitive phenotype, enhanceable by skl^UAS.cWa/Scer\GAL4^GMR.PF

(Wing et al., 2002)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has pigment cell phenotype, enhanceable by Scer\GAL4^GMR.PF/morgue^EP2367

(Wing et al., 2002)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has eye phenotype, enhanceable by SkpA^EP1423/Scer\GAL4^GMR.PF

(Wing et al., 2002)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has pigment cell phenotype, enhanceable by SkpA^EP1423/Scer\GAL4^GMR.PF

(Wing et al., 2002)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has pigment cell phenotype, enhanceable by Scer\GAL4^GMR.PF/Uba1^EP2375

(Wing et al., 2002)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has eye phenotype, non-enhanceable by morgue^ΔFB.UAS/Scer\GAL4^GMR.PF

(Zhou et al., 2013)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has eye phenotype, non-enhanceable by morgue^ΔUEV.UAS/Scer\GAL4^GMR.PF

(Zhou et al., 2013)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has eye phenotype, non-enhanceable by morgue^ΔZF.UAS/Scer\GAL4^GMR.PF

(Zhou et al., 2013)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS, skl^UAS.cWa has eye | heat sensitive phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PF

(Wing et al., 2002)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has eye phenotype, suppressible by SkpA^EP1216a/EP1216b^EP1216b, Scer\GAL4^GMR.PF

(Wing et al., 2002)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has eye phenotype, suppressible by Df(2L)dp-h25/+, Scer\GAL4^GMR.PF

(Wing et al., 2002)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has eye phenotype, suppressible by Df(2L)morgue¹⁹/+, Scer\GAL4^GMR.PF

(Wing et al., 2002)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has eye phenotype, suppressible by morgue[+]/morgue^EP1184/Scer\GAL4^GMR.PF

(Wing et al., 2002)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has eye phenotype, suppressible by Dronc^C318S.GMR

(Wing et al., 2001)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has eye phenotype, suppressible by Diap1^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Wing et al., 2001)

Scer\GAL4^GMR.PF, grim::rpr^rg.UAS has eye phenotype, suppressible | partially by Diap2^UAS.cWa, Scer\GAL4^GMR.PF

(Wing et al., 2001)

Other

Statement

Reference

Scer\GAL4^Gp150-52A, grim::rpr^rg.UAS, rpr^UAS.cZa has ventral midline of embryo phenotype

(Wing et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of morgue^Scer\UAS.cZa enhances the level of eye cell death seen when grim::rpr^rg.Scer\UAS is expressed under the control of Scer\GAL4^GMR.PF.

Expression of morgue^{ΔZF.Scer\UAS} does not enhance the level of eye cell death seen when grim::rpr^rg.Scer\UAS is expressed under the control of Scer\GAL4^GMR.PF.

Expression of morgue^{ΔFB.Scer\UAS} does not enhance the level of eye cell death seen when grim::rpr^rg.Scer\UAS is expressed under the control of Scer\GAL4^GMR.PF.

Expression of morgue^{ΔUEV.Scer\UAS} does not enhance the level of eye cell death seen when grim::rpr^rg.Scer\UAS is expressed under the control of Scer\GAL4^GMR.PF.

Expression of morgue^{G421C.Scer\UAS} enhances the level of eye cell death seen when grim::rpr^rg.Scer\UAS is expressed under the control of Scer\GAL4^GMR.PF.

Expression of morgue^{G421A.Scer\UAS} enhances the level of eye cell death seen when grim::rpr^rg.Scer\UAS is expressed under the control of Scer\GAL4^GMR.PF.

Expression of morgue^{G421S.Scer\UAS} enhances the level of eye cell death seen when grim::rpr^rg.Scer\UAS is expressed under the control of Scer\GAL4^GMR.PF.

Expression of morgue^{Scer\UAS.N.T:Zzzz\FLAG} enhances the level of eye cell death seen when grim::rpr^rg.Scer\UAS is expressed under the control of Scer\GAL4^GMR.PF.

Expression of morgue^{Scer\UAS.C.T:Zzzz\FLAG} enhances the level of eye cell death seen when grim::rpr^rg.Scer\UAS is expressed under the control of Scer\GAL4^GMR.PF.

(Zhou et al., 2013)

The addition of skl^Scer\UAS.cWa, to grim::rpr^rg.Scer\UAS, Scer\GAL4^GMR.PF animals leads to complete lethality. At 18^oC there are a few escapers that exhibit severe eye loss of eye tissue. The subsequent addition of BacA\p35^Scer\UAS.cHa represses these phenotypes: The animals are viable and eyes exhibit essentially normal eye size and pigmentation.

(Wing et al., 2002)

Co-expression of Uba1^EP2375 enhances the eye phenotype caused by expression of grim::rpr^rg.Scer\UAS under the control of Scer\GAL4^GMR.PF; eye pigmentation is reduced. Co-expression of skpA^EP1423 enhances the eye phenotype caused by expression of grim::rpr^rg.Scer\UAS under the control of Scer\GAL4^GMR.PF; eye size and/or pigmentation is reduced and the combination shows semi-lethality. Co-expression of morgue^EP2367 enhances the eye phenotype caused by expression of grim::rpr^rg.Scer\UAS under the control of Scer\GAL4^GMR.PF; eye pigmentation is reduced. The eye phenotype caused by expression of grim::rpr^rg.Scer\UAS under the control of Scer\GAL4^GMR.PF is suppressed by the "EP1216" line, which contains two P{EP} insertions; P{EP}Suv4-20^EP1216a and P{EP}EP1216b^EP1216b. The eye cell death phenotype caused by expression of grim::rpr^rg.Scer\UAS under the control of Scer\GAL4^GMR.PF is suppressed by heterozygosity for Df(2L)dp-h25, morgue^EP1184 or Df(2L)morgue¹⁹.

(Wing et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

grim::rpr^rg.Scer\UAS

grim::rpr^rg.UAS

Name Synonyms

Secondary FlyBase IDs

References (4)

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