FB2024_03 , released June 25, 2024
Allele: Dmel\sliGA20
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General Information
Symbol
Dmel\sliGA20
Species
D. melanogaster
Name
FlyBase ID
FBal0123697
Feature type
allele
Associated gene
Dmel\sli
Associated Insertion(s)
Carried in Construct
Also Known As
slitGA20
Key Links
Genomic Maps

Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Battye et al., 2001)
Mutagen
ethyl methanesulfonate
(Battye et al., 2001)
Progenitor genotype
Cytology
Description

Deletion of one base in G-Domain results in a frameshift and immediate translation termination.

(Battye et al., 2001)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
2R:15,876,192..15,876,192 [-]
Amino acid change:

L837term | sli-PC, L813term | sli-PB, L813term | sli-PA

Reported amino acid change:

L?term

Comment:

A one base deletion leads to early translation termination.

(Battye et al., 2001)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      In stage 16 sliGA20 mutant embryos 46% of the ganglionic branches cross the midline and the rest are either stalled outside the ventral nerve cord or misrouted inside it.

      (Gallio et al., 2004)

      1.9% of expected mutant embryos hatch as first instar larvae. The latest observed stage of mutants is L1. Nerve cord length is 90% of embryo length. Midline guidance failure of repellence phenotype, evidenced by midline crossing, is severe.

      (Battye et al., 2001)

      The GMC-1 in mutants frequently (about 10%) divide symmetrically to generate two RP2s instead of an RP2 and a sib. The RP2 and aCC pCC and other neurons are occasionally misplaced within a hemisegment of the CNS of 13-hour or older mutant embryos, however in younger embryos these cells are not displaced and do not cross the midline or segmental boundary.

      (Mehta and Bhat, 2001)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      sliGA20 has larval ventral nerve cord phenotype, enhanceable by robo[+]/robo11

      (Battye et al., 2001)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      23.8% of dMP2 neurons project contralaterally in sliGA20/robo1 transheterozygous embryos. This phenotype is suppressed by expression of sliScer\UAS.cKa under the control of Scer\GAL4605.

      (Hiramoto and Hiromi, 2006)

      The effects of sliGA20 and ruPLLb on migration of ganglionic branch trachea approaching the ventral midline during stage 16 appear to be additive.

      (Gallio et al., 2004)

      Percentage of segments with midline crossovers in robo1/sliGA20 transheterozygotes is 60%.

      (Battye et al., 2001)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Protein null.

      (Battye et al., 2001)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      sliGA20
      (MacMullin and Jacobs, 2006)
      slitGA20
      (Hiramoto and Hiromi, 2006, Battye et al., 2001)
      Name Synonyms
      Secondary FlyBase IDs
        References (6)