FlyBase Allele Report: Dmel\Fas2[EP1462]

General Information

Symbol

Dmel\Fas2^EP1462

Species

D. melanogaster

Name

FlyBase ID

FBal0123172

Feature type

allele

Associated gene

Dmel\Fas2

Associated Insertion(s)

P{EP}Fas2^EP1462

Carried in Construct

Also Known As

fasII^EP1462, EP(X)1462

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Kraut et al., 2001, Kraut et al., 2001)

Progenitor genotype

Associated Insertion(s)

P{EP}Fas2^EP1462

Cytology

Description

The P{EP} element is inserted 96 nucleotides 5' to the cDNA start.

(Kraut et al., 2001)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

X:4,206,129..4,206,129 [-]

(Buff et al., 2007, Pascual et al., 2005, Gene Disruption Project members, 2001-, Kraut et al., 2001, Kraut et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

Phenotype Manifest In

adult Malpighian tubule, with Scer\GAL4^CapaR.1.5

(Halberg et al., 2016)

adult Malpighian tubule, with Scer\GAL4^Uro.PT

(Halberg et al., 2016)

anterior fascicle & synapse, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

anterior fascicle & synapse | supernumerary, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

brush border | adult stage, with Scer\GAL4^Uro.PT

(Halberg et al., 2016)

larval intersegmental nerve, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

microvillus | adult stage, with Scer\GAL4^CapaR.1.5

(Halberg et al., 2016)

microvillus | adult stage, with Scer\GAL4^Uro.PT

(Halberg et al., 2016)

Detailed Description

Statement

Reference

Expression of Fas2^EP1462 under the control of Scer\GAL4^Uro.PT leads to denser and longer microvillar brush border; a significant increase in microvilli length; larger bundles of microvilli with a significant decrease in intermicrovillar distance; and a significant increase in the stimulated rate of fluid secretion in renal tubules compared to control adults.

(Halberg et al., 2016)

Mutant larvae expressing Fas2^EP1462 under the control of Scer\GAL4^elav-C155 have ISN neuron pathfinding defects. Mutant larvae expressing Fas2^EP1462 under the control of Scer\GAL4^elav-C155 have excessive or ectopic synapses. Mutant larvae expressing Fas2^EP1462 under the control of Scer\GAL4^elav-C155 have reduced or abnormal synapses.

(Kraut et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Fas2^EP1462, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Buff et al., 2007)

Other

Statement

Reference

Phenotype Manifest In

Suppressor of

Statement

Reference

Fas2^EP1462, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Buff et al., 2007)

Other

Statement

Reference

Additional Comments

Genetic Interactions

Statement

Reference

Third instar larvae with co-expression of GluRIIC^{dsRNA.Scer\UAS.cBa} and Fas2^EP1462 driven simultaneously by Scer\GAL4^elav-C155, Scer\GAL4^sca.PC and Scer\GAL4^C57 show significant decreases in mEPSP (decreasing quantal size) and show significant (though not complete) impairment of compensatory homeostatic increases in quantal content (compared to quantal content increases seen with expression of GluRIIC^{dsRNA.Scer\UAS.cBa} alone) at the NMJ.

Third instar GluRIIA^SP16/GluRIIA^SP16 larvae with expression of Fas2^EP1462 driven by Scer\GAL4^C57 show significant decreases in mEPSP (decreasing quantal size) and show significant (though not complete) impairment of compensatory homeostatic increases in quantal content (compared to quantal content increases seen in GluRIIA^SP16/GluRIIA^SP16 mutants alone) at the NMJ; GluRIIA^SP16/GluRIIA^SP16 larvae with Fas2^EP1462 expression driven by Scer\GAL4^elav-C155 show similar decreases in mEPSP and increases in quantal content as GluRIIA^SP16/GluRIIA^SP16 mutants alone.

Third instar GluRIIA^SP16/GluRIIA^SP16 larvae with co-expression of Fas2^EP1462 and Csk^{Scer\UAS.T:Avic\GFP-YFP} driven by Scer\GAL4^C57 show significant decreases in mEPSP (decreasing quantal size) and show complete impairment of compensatory homeostatic increases in quantal content (compared to significant quantal content increases seen in GluRIIA^SP16/GluRIIA^SP16 mutants alone) at the NMJ.

(Spring et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11231

w¹¹¹⁸ P{EP}Fas2^EP1462

Notes on Origin

Discoverer

Selected as: an insertion line that modifies the eye phenotype caused by expression of Ppt1^Scer\UAS.cKa under the control of Scer\GAL4^GMR.PF when co-expressed using Scer\GAL4^GMR.PF.

(Buff et al., 2007)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Fas2^EP1462

Fas2EP

(Halberg et al., 2016)

FasII^EP1462

(Spring et al., 2016)

fas2^EP1462

(Johnson et al., 2012)

fasII^EP1462

(Pascual et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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