FlyBase Allele Report: Dmel\Ras85D[x7b]

General Information

Symbol

Dmel\Ras85D^x7b

Species

D. melanogaster

Name

FlyBase ID

FBal0123112

Feature type

allele

Associated gene

Dmel\Ras85D

Associated Insertion(s)

Carried in Construct

Also Known As

ras^x7b

Key Links

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Halfar et al., 2001)

Mutagen

P-element activity

(Halfar et al., 2001)

Progenitor genotype

Ras85D⁰⁵⁷⁰³

(Halfar et al., 2001)

Cytology

Description

Deletion of Ras85D starting 18bp upstream of the transcription start and ending 198bp downstream of the putative polyadenylation signal.

(Halfar et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible | recessive | somatic clone

(Halfar et al., 2001)

visible | somatic clone, with Ras85D^D38E

(Halfar et al., 2001)

Phenotype Manifest In

Detailed Description

Statement

Reference

Ras85D^x7b mutant eye disc clones in a minute background do not show any differentiation of ommatidial cells.

(Mavromatakis and Tomlinson, 2012)

Ras85D^x7b homozygous clones in the dorsal air sac primordium grow more slowly than wild-type clones and never contribute to the tip of the primordium.

(Cabernard and Affolter, 2005)

Ras85D^x7b clones induced in the wing disc in the first larval instar can be recovered in the prospective wing blade domain, wing hinge and medial notum, but are generally not seen in the prospective lateral notum domain.

(Zecca and Struhl, 2002)

Homozygous clones are not recovered in the adult eye. Homozygous clones are seen in the eye imaginal disc, but the size of the clone is reduced compared to the wild-type sister clone (twin spot). Clones in the eye behind the morphogenetic furrow are often wedge shaped and much reduced in size. Clusters of apoptotic cells are seen in clones posterior to the morphogenetic furrow. Homozygous clones in the eye which are Minute+ (in a RpS3² background) are substantially larger and cover large areas of the disc. As seen in a wild-type background, the size of these clones is reduced behind the morphogenetic furrow. Flies homozygous for Ras85D^x7b in the eye disc develop with a severely reduced head that almost completely lacks eyes. Introduction of Ras85D^D38E into flies that are homozygous for Ras85D^x7b in the eye disc results in a slight enlargement of the eye field, due to the presence of unpigmented cuticle, but no additional ommatidial structures are formed.

(Halfar et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Ras85D^D38E, Ras85D^x7b has visible | somatic clone phenotype, suppressible by rl[+]/rl^Sem

(Halfar et al., 2001)

NOT suppressed by

Statement

Reference

Ras85D^x7b has visible | recessive | somatic clone phenotype, non-suppressible by rl[+]/rl^Sem

(Halfar et al., 2001)

Phenotype Manifest In

Suppressed by

Statement

Reference

Ras85D^D38E, Ras85D^x7b has adult head | somatic clone phenotype, suppressible by rl[+]/rl^Sem

(Halfar et al., 2001)

Ras85D^D38E, Ras85D^x7b has eye | somatic clone phenotype, suppressible by rl[+]/rl^Sem

(Halfar et al., 2001)

NOT suppressed by

Statement

Reference

Ras85D^x7b has eye | somatic clone phenotype, non-suppressible by rl[+]/rl^Sem

(Halfar et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

rl^Sem/+ alone is not sufficient to rescue the reduced eye and head phenotypes of flies that are homozygous for Ras85D^x7b in the eye disc. However, the addition of Ras85D^D38E in combination with rl^Sem results in a substantial rescue of the Ras85D^x7b eye and head size phenotypes; differentiated photoreceptor cells are seen and a substantial fraction of the ommatidia contain the full complement of photoreceptors.

(Halfar et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Ras85D^x7b is rescued by Ras85D^+t3.6

(Halfar et al., 2001)

Partially rescued by

Ras85D^x7b is partially rescued by Ras85D^D38E

(Halfar et al., 2001)

Not rescued by

Ras85D^x7b is not rescued by Ras85D^Y40C

(Halfar et al., 2001)

Comments

Ras85D^D38E partially rescues the growth defects of Ras85D^x7b clones in the eye disc. In addition, the rescued cells survive beyond the morphogenetic furrow and R8 photoreceptor cells are seen to develop in the clone. The partial rescue is not increased by increased Ras85D^D38E copy number nor by the addition of Ras85D^Y40C.

(Halfar et al., 2001)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Ras85D^x7b

Ras^x7b

(Halfar et al., 2001)

ras85D^x7b

(Auer et al., 2015)

ras^x7b

(Mavromatakis and Tomlinson, 2012, Cabernard and Affolter, 2005, Zecca and Struhl, 2002)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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