FB2024_03 , released June 25, 2024
Allele: Dmel\Ras85D05703
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General Information
Symbol
Dmel\Ras85D05703
Species
D. melanogaster
Name
FlyBase ID
FBal0042723
Feature type
allele
Associated gene
Dmel\Ras85D
Associated Insertion(s)
P{PZ}Ras85D05703
Carried in Construct
Also Known As
Ras15703, ras5703
Key Links
Genomic Maps

Allele class

hypomorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - genetic evidence

(Rorth, 1996, Schnorr and Berg, 1996)
Mutagen
P-element activity
(Rorth, 1996, Schnorr and Berg, 1996)
Progenitor genotype
Associated Insertion(s)
P{PZ}Ras85D05703
Cytology
Description

P{PZ} insertion is located 28bp upstream of a putative transcription start site as determined by cDNA analysis.

(Schnorr and Berg, 1996)
Allele components
Component
Use(s)
Inserted element
P{PZ}
Encoded product / tool
lacZ
Tagged with
Tag:NLS(P)
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      100% of the eggs laid by Ras85D05703/Ras85De1B mothers have a single central dorsal appendage.

      (Ward and Berg, 2005)

      The Ras85D05703 mutant result in a significant reduction in the number of type I synaptic boutons compared with wild-type.

      (Koh et al., 2002)

      Homozygotes have no detectable eye defects.

      (Rorth, 1996)

      A low percentage of egg chambers from homozygous females degenerate between stages 8 and 10. Some egg chambers contain too few nurse cells or fail to correctly position the developing oocyte within the egg chamber. Female transheterozygotes with Df(3R)by10 have rudimentary ovaries. All late stage egg chambers lack dorsal appendage structures and possess only a patch of chorion centered on the dorsal midline. Few eggs are laid, eggs appear ventralised but their overall shape is normal. Homozygous eyes exhibit slightly disrupted ommatidial array. Ras85DΔC17b/Ras85D05703 and Df(3R)ΔC40b/Ras85De1B transheterozygotes are lethal. Ras85De1B/Ras85D05703 and Ras85De2F/Ras85D05703 transheterozygotes are semi-lethal. Ras85DSutor-341/Ras85D05703 and Ras85DSutor-404/Ras85D05703 transheterozygotes have low viability. Female transheterozygotes with Ras85De2F, Ras85DSutor-341 or Ras85DSutor-404 lay eggs with a severely disrupted eggshell (patch of chorion instead of dorsal appendages), 57%, 40% and 25% of eggs (respectively) hatch and develop to normal larvae. Unhatched Ras85D05703/Ras85De2F embryos have normal cuticles, or are missing elements of the head skeleton or have defects in the anterior and posterior termini, holes in the cuticle and disordered posterior segments. Female transheterozygotes with Ras85De1B lay eggs with a single dorsal appendage, 84% of eggs hatch and develop to normal larvae. Transheterozygotes with Ras85De2F, Ras85DSutor-341 or Ras85DSutor-404 exhibit slightly disrupted ommatidial array (small number of fused and misplaced ommatidia). Transheterozygotes with Ras85De1B exhibit extremely rough eyes (many fused and misplaced ommatidia).

      (Schnorr and Berg, 1996)

      female-sterile.

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Statement
      Reference

      Ras85D05703 is an enhancer of visible phenotype of RasGAP1EP45, Scer\GAL4hs.2sev

      (Rorth, 1996)
      Phenotype Manifest In
      NOT suppressed by
      Statement
      Reference

      Ras85D05703 has phenotype, non-suppressible by Rlb1hs.PE

      (Schnorr and Berg, 1996)
      Enhancer of
      Statement
      Reference

      Ras85D05703/Ras85D[+] is an enhancer of heart primordium phenotype of sli2

      (MacMullin and Jacobs, 2006)

      Ras85D05703 is an enhancer of eye phenotype of RasGAP1EP45, Scer\GAL4hs.2sev

      (Rorth, 1996)
      Suppressor of
      Statement
      Reference

      Ras85D05703/Ras85D[+] is a suppressor of phenotype of Src42ASu(Raf)1-1

      (Zhang et al., 1999)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Ras85D05703/+; sli2/+ embryos produce a mild heart development phenotype.

      (MacMullin and Jacobs, 2006)

      Dominantly suppresses the ability of Src42ASu(phl)1-1 to suppress the lethality of phl1/Y flies.

      (Zhang et al., 1999)

      Expression of Gap1EP45 in homozygous Ras85D05703 flies using Scer\GAL4hs.2sev produces severely disorganised eyes.

      (Rorth, 1996)

      Expression of Rlb1hs.PE fails to ameliorate the phenotype.

      (Schnorr and Berg, 1996)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Ras85D05703 is rescued by Ras85D+t5.5

      (Schnorr and Berg, 1996)
      Not rescued by

      Ras85D05703 is not rescued by Ras85DΔ

      (Schnorr and Berg, 1996)
      Comments

      Mutant phenotypes are completely rescued by Ras85D+t5.5, restoring viability and proper ovarian development. Ras85DΔ fails to rescue the mutant phenotypes.

      (Schnorr and Berg, 1996)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      A. Spradling.

      Comments
      Comments

      Complements: ms(3)85D03565.

      (BDGP Project Members, 1994-1999)

      Precise excision of the P{PZ} insertion reverts the lethal and female sterile phenotypes demonstrating the insertion is responsible for the mutant phenotype.

      (Schnorr and Berg, 1996)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (8)
      Reported As
      Symbol Synonym
      85D5703
      Ras15703
      (Yakoby et al., 2008, Zhang et al., 1999, Mantrova and Hsu, 1998, Rorth, 1996, Schnorr and Berg, 1996)
      Ras85D05703
      (Mahoney et al., 2006)
      Ras85D05703
      (Zartman et al., 2008)
      RasP5703
      (Koh et al., 2002)
      fs(3)0570305703
      fs(3)5703
      ras5703
      (Knox et al., 2011, MacMullin and Jacobs, 2006, Settle et al., 2003)
      Name Synonyms
      Secondary FlyBase IDs
        References (17)