FB2024_03 , released June 25, 2024
Allele: Dmel\robo21
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General Information
Symbol
Dmel\robo21
Species
D. melanogaster
Name
FlyBase ID
FBal0121562
Feature type
allele
Associated gene
Dmel\robo2
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Sano et al., 2005, Rajagopalan et al., 2000)
Mutagen
ethyl methanesulfonate
(Rajagopalan et al., 2000)
Progenitor genotype
robo2EP2582
(Rajagopalan et al., 2000)
Cytology
Description

Amino acid replacements are a revision of the data reported in FBrf0132258. They are numbered relative to the 1406 amino acid residue predicted protein.

(Dickson, 2001.3.14)

Amino acid replacement: R384term.

(Dickson, 2001.3.14)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2L:1,392,318..1,392,318 [-]
Nucleotide change:

C1392318T

Amino acid change:

R441term | robo2-PA; R441term | robo2-PB

Reported amino acid change:

R441term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Rajagopalan et al., 2000)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      learobo2-1 embryos show weak salivary gland guidance defects.

      (Kolesnikov and Beckendorf, 2005)

      learobo2-1/learobo2-5 and learobo2-1/learobo2-4 have defects in the ellipsoid body and fan-shaped body.

      16% of mushroom bodies lack dorsal projections in learobo2-1/learobo2-5 animals, and 32% have a partial median projection. In addition, the diameter of the peduncle is larger than in wild-type animals.

      34% of mushroom bodies lack dorsal projections in learobo2-4/learobo2-1 animals, and 18% have a partial median projection. The median lobes are positioned more posteriorly than in wild type. In addition, the the diameter of peduncle is larger than in wild-type animals.

      (Nicolas and Preat, 2005)

      learobo2-1/learobo2-1 DM2 glomerulus innervating neurons, produced by mitotic recombination in a learobo2-1/+ background, fail to cross over to the contralateral lobe through the antennal commissure. Instead they terminate at the midline forming small "glomerular-like" structures. Targeting of these neurons to antennal glomerulus DM2 occurs normally, although in many cases the glomeruli appear less intensely innervated (13/16).

      (Jhaveri et al., 2004)

      The lateral Fas2-expressing fascicle often merges with the intermediate fascicle in learobo2-1/learobo2-4 mutant embryos. Midline crossing defects also occur. 7.9% of embryos show fusion between the medial and intermediate fascicles, 17.1% show fusion between the lateral and and intermediate fascicles and 13.4% show breaks in the lateral fascicle.

      (Rajagopalan et al., 2000)

      In learobo2-1/learobo2-4 mutants about a quarter of Fas2 positive axons in the ventral nerve cord are misrouted. These form bundles of varying thickness.

      (Rajagopalan et al., 2000)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      robo21 has presumptive embryonic salivary gland phenotype, enhanceable by robo11

      (Kolesnikov and Beckendorf, 2005)
      Enhancer of
      Statement
      Reference

      robo21 is an enhancer of presumptive embryonic salivary gland phenotype of robo11

      (Kolesnikov and Beckendorf, 2005)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      In robo1 learobo2-1 double mutants, the salivary gland guidance phenotype is enhanced. Glands are curved medially towards the midline, instead of lying parallel to the midline as in wild type.

      (Kolesnikov and Beckendorf, 2005)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      References (11)