FlyBase Allele Report: Dmel\Rab7[UAS.GFP]

General Information

Symbol

Dmel\Rab7^UAS.GFP

Species

D. melanogaster

Name

FlyBase ID

FBal0120653

Feature type

allele

Associated gene

Dmel\Rab7

Associated Insertion(s)

Carried in Construct

P{UAS-Rab7.GFP}

Also Known As

UAS-Rab7-GFP, UAS-GFP-Rab7, Rab7-GFP, UAS-GFP:Rab7, GFP-Rab7, UAS-Rab7::GFP, UAS-Rab7GFP, Rab7GFP, UASrab7-GFP, UAS-Rab7.GFP

Key Links

Transgenic product class

wild_type

(Entchev et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Entchev et al., 2000)

Mutagen

in vitro construct

(Entchev et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-Rab7.GFP}

Cytology

Description

UAS regulatory sequences drive expression of Rab7 tagged at the N-terminal end with GFP.

(Entchev et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Rab7

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by Hsap\SNCA^A53T.UAS

(Dinter et al., 2016)

exacerbates Alzheimer's disease 1

modeled by Hsap\APP^2xAPP.SP.UAS

(Yu et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

late endosome, with Scer\GAL4^unspecified

(Rives et al., 2006)

Detailed Description

Statement

Reference

Expression of Rab7^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^GMR.PFa does not perturb the structure or appearance of the adult eye compared to controls.

(Ruan et al., 2016)

Wing discs expressing Rab7^{Scer\UAS.T:Avic\GFP} contain enlarged late endosomes.

(Rives et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Rab7^UAS.GFP, Scer\GAL4^{elav.Switch.PO} is an enhancer of short lived | RU486 conditional phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^{elav.Switch.PO}

(Yu et al., 2020)

NOT Enhancer of

Statement

Reference

Rab7^UAS.GFP, Scer\GAL4^ninaE.PT is a non-enhancer of decreased cell number | adult stage | progressive phenotype of Hsap\BIN1^UAS.1, Scer\GAL4^ninaE.PT

(Lambert et al., 2022)

Suppressor of

Statement

Reference

Rab7^UAS.GFP, Scer\GAL4^elav.PLu is a suppressor of abnormal locomotor behavior | adult stage | progressive phenotype of Hsap\SNCA^A53T.UAS, Scer\GAL4^elav.PLu

(Dinter et al., 2016)

Rab7^UAS.GFP, Scer\GAL4^GMR.PFa is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^GMR.PFa, egr^UAS.cMa

(Ruan et al., 2016)

NOT Suppressor of

Statement

Reference

Rab7^UAS.GFP, Scer\GAL4^ninaE.PT is a non-suppressor of decreased cell number | adult stage | progressive phenotype of Hsap\BIN1^UAS.1, Scer\GAL4^ninaE.PT

(Lambert et al., 2022)

Phenotype Manifest In

Enhancer of

Statement

Reference

Rab7^UAS.GFP, Scer\GAL4^Dll-md23 is an enhancer of leg phenotype of RhoGAP68F^UAS.mCherry, Scer\GAL4^Dll-md23

(de Madrid et al., 2015)

Rab7^UAS.GFP, Scer\GAL4^Dll-md23 is an enhancer of epithelium phenotype of RhoGAP68F^UAS.mCherry, Scer\GAL4^Dll-md23

(de Madrid et al., 2015)

NOT Enhancer of

Statement

Reference

Rab7^UAS.GFP, Scer\GAL4^ninaE.PT is a non-enhancer of outer photoreceptor cell | adult stage | decreased number phenotype of Hsap\BIN1^UAS.1, Scer\GAL4^ninaE.PT

(Lambert et al., 2022)

Suppressor of

Statement

Reference

Rab7^UAS.GFP, Scer\GAL4^GMR.PFa is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PFa, egr^UAS.cMa

(Ruan et al., 2016)

NOT Suppressor of

Statement

Reference

Rab7^UAS.GFP, Scer\GAL4^ninaE.PT is a non-suppressor of outer photoreceptor cell | adult stage | decreased number phenotype of Hsap\BIN1^UAS.1, Scer\GAL4^ninaE.PT

(Lambert et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

The dot-like small eye phenotype characteristic for flies expressing egr^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PFa is partially suppressed by co-expression of Rab7^{Scer\UAS.T:Avic\GFP}.

(Ruan et al., 2016)

Co-expression of Rab7^{Scer\UAS.T:Avic\GFP} dramatically increases the incidence of tears in the leg epithelium in animals with moderate expression of RhoGAP68F^{Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^Dll-md23 (penetrance increases from 12% to 74%).

(de Madrid et al., 2015)

Xenogenetic Interactions

Statement

Reference

Expression of Rab7^{Scer\UAS.T:Avic\GFP} does not lead to a significant difference in the the locomotion activity of flies expressing Hsap\SNCA^{A53T.Scer\UAS} under the control of Scer\GAL4^elav.PLu at 5 days old, however, at 15 and 25 days old results in significantly improved locomotion, delaying the onset of locomotion defects seen in flies expressing Hsap\SNCA^{A53T.Scer\UAS} under the control of Scer\GAL4^elav.PLu.

(Dinter et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

42705

w^*; P{UAS-Rab7.GFP}2

42706

w^*; P{UAS-Rab7.GFP}3