FlyBase Allele Report: Dmel\Cdk4[D175N.UAS.Tag:MYC]

General Information

Symbol

Dmel\Cdk4^{D175N.UAS.Tag:MYC}

Species

D. melanogaster

Name

FlyBase ID

FBal0119690

Feature type

allele

Associated gene

Dmel\Cdk4

Associated Insertion(s)

Carried in Construct

P{UAS-Cdk4^D175N-myc}

Key Links

Transgenic product class

missense_variant

(Meyer et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Meyer et al., 2000)

Mutagen

in vitro construct

(Meyer et al., 2000)

Progenitor genotype

Cdk4^UAS.Tag:MYC

(Meyer et al., 2000)

Carried in construct

P{UAS-Cdk4^D175N-myc}

Cytology

Description

Construct: Amino acid replacement: D175N.

(Meyer et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Cdk4

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size, with Scer\GAL4^da.G32

(Meyer et al., 2000)

Phenotype Manifest In

abdominal 4 ventral denticle belt, with Scer\GAL4^{VP16.mat.αTub67C}

(Chen et al., 2003)

ventral denticle belt, with Scer\GAL4^{VP16.mat.αTub67C}

(Chen et al., 2003)

Detailed Description

Statement

Reference

Cdk4^{D175N.Scer\UAS.T:Hsap\MYC}; Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} embryos have segmentation defects, such as loss of denticle belt A4, and abnormal formation of other denticle belts.

(Chen et al., 2003)

When driven by Scer\GAL4^hs.2sev, Scer\GAL4^GMR.PF or Scer\GAL4^ey.PH, no eye phenotype is seen.

(Datar et al., 2000)

The addition of Cdk4^{D175N.Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^da.G32 to wild-type flies lead to a slight but significant decrease in weight.

(Meyer et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^ey.PH, CycD^UAS.cDa/Cdk4^{D175N.UAS.Tag:MYC} is a suppressor | partially of eye phenotype of Rbf^UAS.cDa, Scer\GAL4^ey.PH

(Datar et al., 2000)

NOT Suppressor of

Statement

Reference

Cdk4^{D175N.UAS.Tag:MYC}, Scer\GAL4^ey.PH is a non-suppressor of eye phenotype of Rbf^UAS.cDa, Scer\GAL4^ey.PH

(Datar et al., 2000)

Other

Statement

Reference

Cdk4^{D175N.UAS.Tag:MYC}, CycD^UAS.cDa, Scer\GAL4^hs.2sev has eye phenotype

(Datar et al., 2000)

Cdk4^{D175N.UAS.Tag:MYC}, CycD^UAS.cDa, Scer\GAL4^GMR.PF has eye phenotype

(Datar et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

When Cdk4^{D175N.Scer\UAS.T:Hsap\MYC} and CycD^Scer\UAS.cDa are driven by Scer\GAL4^ey.PH no eye phenotype is seen. When either Scer\GAL4^hs.2sev or Scer\GAL4^GMR.PF is the driver, no eye overgrowth is seen, though a slight eye phenotype is seen. The addition of Cdk4^{D175N.Scer\UAS.T:Hsap\MYC} to Rbf^Scer\UAS.cDa flies (when driven by Scer\GAL4^ey.PH, Scer\GAL4^hs.2sev or Scer\GAL4^GMR.PF) has nol effect on the eye phenotype. The addition of Cdk4^{D175N.Scer\UAS.T:Hsap\MYC} to Rbf^Scer\UAS.cDa, CycD^Scer\UAS.cDa flies when driven by Scer\GAL4^hs.2sev or Scer\GAL4^GMR.PF produces eyes that are slightly larger than normal. When driven by Scer\GAL4^ey.PH produces eyes that are slightly smaller than seen with Rbf^Scer\UAS.cDa, CycD^Scer\UAS.cDa and Scer\GAL4^ey.PH alone.

(Datar et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

Scer\GAL4^da.G32/Cdk4^{D175N.UAS.Tag:MYC} fails to rescue Cdk4³

(Meyer et al., 2000)

Comments

The addition of Cdk4^Scer\UAS.cMa driven by Scer\GAL4^da.G32 fails to rescue the weight, body and cell size phenotypes seen in Cdk4³ flies.

(Meyer et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Cdk4^{D175N.Scer\UAS.T:Hsap\MYC}

Cdk4^{D175N.UAS.Tag:MYC}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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