FlyBase Allele Report: Dmel\emc[EP3620]

General Information

Symbol

Dmel\emc^EP3620

Species

D. melanogaster

Name

FlyBase ID

FBal0119254

Feature type

allele

Associated gene

Dmel\emc

Associated Insertion(s)

P{EP}emc^EP3620

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Bidet et al., 2003, Pena-Rangel et al., 2002, Gene Disruption Project members, 2001-, Abdelilah-Seyfried et al., 2000)

Progenitor genotype

Associated Insertion(s)

P{EP}emc^EP3620

Cytology

Description

P{EP} insertion 212bp upstream of the emc translation initiation codon. The P{EP} element is oriented such that expression from the element results in overexpression of emc.

(Bidet et al., 2003)

P{EP} insertion at -72bp relative to the start of the transcription unit.

(Abdelilah-Seyfried et al., 2000)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:749,335..749,335 [+]

(Bhattacharya and Baker, 2011, Singh et al., 2005, Adam and Montell, 2004, Bidet et al., 2003, Pena-Rangel et al., 2002, Gene Disruption Project members, 2001-, Abdelilah-Seyfried et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

egg chamber, with Scer\GAL4^c306

(Adam and Montell, 2004)

egg chamber | anterior, with Scer\GAL4^upd1.PU

(Adam and Montell, 2004)

external sensory organ precursor cell | somatic clone, with Scer\GAL4^en.PU

(Bhattacharya and Baker, 2011)

eye disc | somatic clone, with Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Bhattacharya and Baker, 2011)

eye disc anterior to the morphogenetic furrow | somatic clone, with Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Bhattacharya and Baker, 2011)

leg disc anterior compartment | somatic clone, with Scer\GAL4^en.PU

(Bhattacharya and Baker, 2011)

leg sensillum | somatic clone, with Scer\GAL4^en.PU

(Bhattacharya and Baker, 2011)

oocyte, with Scer\GAL4^upd1.PU

(Adam and Montell, 2004)

photoreceptor cell R2 | somatic clone, with Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Bhattacharya and Baker, 2011)

photoreceptor cell R3 | somatic clone, with Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Bhattacharya and Baker, 2011)

photoreceptor cell R4 | somatic clone, with Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Bhattacharya and Baker, 2011)

photoreceptor cell R5 | somatic clone, with Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Bhattacharya and Baker, 2011)

photoreceptor cell R8 | somatic clone, with Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Bhattacharya and Baker, 2011)

polar follicle cell, with Scer\GAL4^upd1.PU

(Adam and Montell, 2004)

polar follicle cell | increased number, with Scer\GAL4^upd1.PU

(Adam and Montell, 2004)

Detailed Description

Statement

Reference

Approximately 46% of eye disc clones expressing emc^EP3620 anterior to the morphogenetic furrow (under the control of Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C}) exhibit missing R8 cells. R8 cells are missing more often close to the posterior margin of the clone. Even when the R8 clones are present, 53% of the ommatidia have one or more cells from the R2, R3, R4 and R5 classes missing or failing to express the transcription factor ro.

Maintaining emc expression in the posterior compartment of leg imaginal discs through expression of emc^EP3620 under the control of Scer\GAL4^en.PU at 23[o]C reduces sensory organ precursor selection and subsequent sensory bristle numbers in the posterior compartment of adult legs by 70%.

(Bhattacharya and Baker, 2011)

Expression of emc^EP3620 under the control of Scer\GAL4^c306 results in egg chambers containing multiple germline cysts with high penetrance. Expression of emc^EP3620 under the control of Scer\GAL4^upd results in polar cells being absent from one end or other of about 20% of egg chambers. The oocyte is sometimes mispositioned (4.7% of egg chambers) and the egg chambers are sometimes round in shape at the anterior end (2% of egg chambers). Extra polar cells are formed in 4.2% of egg chambers. Temperature shift experiments indicate that egg chambers that were at stage 2 or 3 during high levels of expression of emc^EP3620 under the control of Scer\GAL4^upd and were analysed at stage 4-8 show more frequent loss of polar follicle cells, while extra polar follicle cells are most often seen in egg chambers that were at stage 5 during high levels of expression of emc^EP3620 under the control of Scer\GAL4^upd and were analysed at stage 9.

(Adam and Montell, 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

emc^EP3620/Scer\GAL4^ey.PH is an enhancer of visible | dominant phenotype of L²

(Singh et al., 2005)

emc^EP3620/Scer\GAL4^pnr-MD237 is an enhancer of visible phenotype of pnr^MD237

(Pena-Rangel et al., 2002)

Phenotype Manifest In

Enhancer of

Statement

Reference

emc^EP3620/Scer\GAL4^ey.PH is an enhancer of eye phenotype of L²

(Singh et al., 2005)

emc^EP3620/Scer\GAL4^pnr-MD237 is an enhancer of chaeta phenotype of pnr^MD237

(Pena-Rangel et al., 2002)

Other

Statement

Reference

Scer\GAL4^pnr-MD237, emc^EP3620, pnr^MD237/pnr[+] has scutellar bristle phenotype

(Pena-Rangel et al., 2002)

Scer\GAL4^pnr-MD237, emc^EP3620, pnr^MD237/pnr[+] has dorsocentral bristle phenotype

(Pena-Rangel et al., 2002)

Scer\GAL4^pnr-MD237, emc^EP3620, pnr^MD237 has scutellar bristle phenotype

(Pena-Rangel et al., 2002)

Scer\GAL4^pnr-MD237, emc^EP3620, pnr^MD237 has dorsocentral bristle phenotype

(Pena-Rangel et al., 2002)

Scer\GAL4^pnr-MD237, emc^EP3620, pnr^MD237 has microchaeta phenotype

(Pena-Rangel et al., 2002)

Scer\GAL4^pnr-MD237, emc^EP3620, pnr^MD237/pnr[+] has microchaeta phenotype

(Pena-Rangel et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

pnr^MD237/+ adults expressing emc^EP3620 under the control of Scer\GAL4^pnr-MD237 show complete loss of dorsocentral and scutellar macrochaetae and microchaetae in the dorsal midline including the abdomen.

(Pena-Rangel et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: a P{EP} insertion line that modifies the pnr^MD237/+ phenotype when expressed using Scer\GAL4^pnr-MD237.

(Pena-Rangel et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

EP(3)3620

(Abdelilah-Seyfried et al., 2000)

emc^EP3620

(Bhattacharya and Baker, 2011)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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