indirect flight muscle motor neuron MN4 & bouton | ectopic
hiwEMS mutant C4da neuronal clones do not exhibit defects in dendrite pruning or neuronal cell death 18-20 hours after puparium formation.
The number of boutons per muscle surface area at the neuromuscular junction is increased in homozygotes compared to wild type.
No defects are seen in axon pruning during metamorphosis, though axon misguidance and branching defects are seen when somatic clones are made in the neuroblast.
In hiwEMS hemizygous larvae MN4a and MN4b motor terminals are present on muscle 4 and muscle 6/7 but contain many more branches and boutons. The number of boutons per muscle was also at least double that seen in wild-type. Synapses also have a 2-fold increase in synaptic size compared to wild-type (after correction for muscle area). The synapses on muscle 12 in mutants are larger in terms of total length, the length of individual branches and the number of branches per synapse. The increase in bouton number is seen even 30 minutes after hatching. Initial axon pathfinding and pathfinding appears normal. A normal pattern of axon pathways, muscle innervation and specificity of contacts are seen. Bouton ultrastructure appears normal. The mean amplitude of spontaneous miniature junctional potentials (mEJPs) is slightly reduced in hiwEMS mutants (about 0.65mV as compared to 0.91mV in wild-type). The evoked release is greatly reduced; the mutant shows a 66% decrease compared to wild-type (about 7.75mV versus 1.72mV in wild-type). This indicates a significant reduction in quantal content and thus a reduction in presynaptic function.
hiwEMS has abnormal neuroanatomy phenotype, enhanceable by tkvQ199D.UAS.Tag:HA/Scer\GAL4elav.PLu
hiwEMS has abnormal neuroanatomy phenotype, suppressible by MedC246/MedC246
hiwEMS has abnormal neuroanatomy phenotype, suppressible by witHA4
hiwEMS has abnormal neuroanatomy phenotype, suppressible by Medunspecified/Med[+]
hiwEMS is an enhancer of increased cell growth rate phenotype of Atg1UAS.cSa, Scer\GAL4elav.PLu
hiw[+]/hiwEMS is an enhancer of abnormal neuroanatomy phenotype of Atg1UAS.cSa, Scer\GAL4elav.PLu
hiw[+]/hiwEMS is an enhancer of increased cell growth rate phenotype of Atg1UAS.cSa, Scer\GAL4elav.PLu
hiwEMS is an enhancer of abnormal neuroanatomy phenotype of Atg1UAS.cSa, Scer\GAL4elav.PLu
hiwEMS has bouton | increased number phenotype, enhanceable by tkvQ199D.UAS.Tag:HA/Scer\GAL4elav.PLu
hiwEMS has neuromuscular junction phenotype, enhanceable by tkvQ199D.UAS.Tag:HA/Scer\GAL4elav.PLu
hiwEMS has NMJ bouton phenotype, suppressible by Df(3L)pbl-NR/+
hiwEMS has NMJ bouton phenotype, suppressible by +/Df(3L)Exel6279
hiwEMS has NMJ bouton phenotype, suppressible by Df(3L)pbl-X1/+
hiwEMS has bouton | increased number phenotype, suppressible by MedC246/MedC246
hiwEMS has bouton | increased number phenotype, suppressible by witHA4
hiwEMS has bouton | increased number phenotype, suppressible by Medunspecified/Med[+]
hiwEMS has neuromuscular junction phenotype, suppressible by MedC246/MedC246
hiwEMS has neuromuscular junction phenotype, suppressible by witHA4
hiwEMS has neuromuscular junction phenotype, suppressible by Medunspecified/Med[+]
hiw[+]/hiwEMS is an enhancer of NMJ bouton phenotype of Atg1UAS.cSa, Scer\GAL4elav.PLu
hiw[+]/hiwEMS is an enhancer of neuromuscular junction phenotype of Atg1UAS.cSa, Scer\GAL4elav.PLu
hiwEMS is an enhancer of NMJ bouton phenotype of Atg1UAS.cSa, Scer\GAL4elav.PLu
hiwEMS is an enhancer of neuromuscular junction phenotype of Atg1UAS.cSa, Scer\GAL4elav.PLu
A hiwEMS heterozygous or homozygous background significantly enhances the high levels of autophagy and bouton number found upon expression of Atg1Scer\UAS.cSa under the control of Scer\GAL4elav.PLu.
One copy of Medunspecified causes a moderate but significant reduction in the number of boutons per muscle surface area at the neuromuscular junction in hiwEMS homozygotes. The excessive synaptic bouton phenotype seen at the neuromuscular junction in hiwEMS homozygotes is strongly suppressed by MedC246/MedC246. The boutons in the double mutant synapses are smaller than wild type and appear more similar to those seen in hiwEMS single mutants. The excessive synaptic bouton phenotype seen at the neuromuscular junction in hiwEMS homozygotes is strongly suppressed by witHA4. In some cases the synaptic span in these double mutants is still slightly larger than wild type. The boutons in the double mutant synapses are smaller than wild type and appear more similar to those seen in hiwEMS single mutants. Expression of tkvQ199D.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4elav.PLu in a hiwEMS background results in a 30% increase in the number of boutons per muscle surface area at the neuromuscular junction compared to hiwEMS single mutants.
The small eye phenotype caused by co-expression of gigScer\UAS.cTa and Tsc1Scer\UAS.cTa under the control of Scer\GAL4ey.PH is enhanced by hiwEMS.