FlyBase Allele Report: Dmel\acj6[1,3,4.UAS]

General Information

Symbol

Dmel\acj6^1,3,4.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0117613

Feature type

allele

Associated gene

Dmel\acj6

Associated Insertion(s)

Carried in Construct

P{UAS-acj6.1,3,4}

Also Known As

UAS-Acj6(1,3,4)

Key Links

Transgenic product class

wild_type

(Certel et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Certel et al., 2000)

Mutagen

in vitro construct

(Certel et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-acj6.1,3,4}

Cytology

Description

This construct contains exons 1, 3, 4 the long form of exon 5, 6, 7, the short form of exon 8, and exon 9.

(Bai and Carlson, 2010)

UASt regulatory sequences drive expression of the acj6 "1,3,4" isoform.

(Certel et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

acj6

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^sca-537.4

(Certel et al., 2000)

Phenotype Manifest In

abdominal ventral longitudinal muscle 1 & neuromuscular junction, with Scer\GAL4^sca-537.4

(Certel et al., 2000)

abdominal ventral longitudinal muscle 2 & neuromuscular junction, with Scer\GAL4^sca-537.4

(Certel et al., 2000)

abdominal ventral longitudinal muscle 3 & neuromuscular junction, with Scer\GAL4^sca-537.4

(Certel et al., 2000)

abdominal ventral longitudinal muscle 4 & neuromuscular junction, with Scer\GAL4^sca-537.4

(Certel et al., 2000)

anterior fascicle & motor neuron, with Scer\GAL4^sca-537.4

(Certel et al., 2000)

bouton | ectopic, with Scer\GAL4^sca-537.4

(Certel et al., 2000)

neuromuscular junction | ectopic, with Scer\GAL4^sca-537.4

(Certel et al., 2000)

Detailed Description

Statement

Reference

Expression of acj6^{1,3,4.Scer\UAS} under the control of Scer\GAL4^acj6-PG63 in a acj6^PG63/Y background is able to induce a fenchone odor response, indicating activation of Or85e.

(Bai and Carlson, 2010)

The ISNb fascicles correctly leave the nerve cord, defasciculate from the ISN and project to target muscle clefts in nearly all late stage 16/stage 17 embryos expressing acj6^{1,3,4.Scer\UAS} under the control of Scer\GAL4^sca-537.4. However, in 77% of hemisegments with normal initial axon pathfinding, a striking increase in the number of nerve terminal branches and processes arising from each motor axon is seen. In addition, some of these ectopic terminal branches extend and form connections onto inappropriate muscle fibres. Third instar larvae show a 27% increase in the number of boutons at the neuromuscular junction at muscles 6 and 7 and a 22% increase in the number of boutons at muscles 12 and 13.

(Certel et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^acj6-PG63/acj6^1,3,4.UAS partially rescues acj6^PG63

(Bai and Carlson, 2010)

Scer\GAL4^acj6-PG63/acj6^1,3,4.UAS partially rescues acj6⁶

(Bai and Carlson, 2010)

Comments

Expression of acj6^{1,3,4.Scer\UAS} under the control of Scer\GAL4^acj6-PG63 rescues the response of the sensilla pb1A neuron to ethyl acetate, isoamyl acetate, 4-methyl phenol, benzaldehyde and fenchone.

Expression of acj6^{1,3,4.Scer\UAS} under the control of Scer\GAL4^acj6-PG63 in a acj6^PG63/Y background is able to induce a fenchone odor response, indicating activation of Or85e.

Expression of acj6^{1,3,4.Scer\UAS} under the control of Scer\GAL4^acj6-PG63 in a acj6^PG63/Y background suppresses the (pb2C, 0) type of sensilla normally found in acj6^PG63 mutants. Expression of acj6^J.Scer\UAS, but not acj6^1,4.Scer\UAS or acj6^{1,3,4.Scer\UAS}, is able to induce normal (pb2A, pb2B) sensilla.

Expression of acj6^{1,3,4.Scer\UAS} under the control of Scer\GAL4^acj6-PG63 in a acj6^PG63/Y background induces two unexpected types of sensilla. One type contains a normal pb2B cell and a novel ORN that responds to 3-octanol but to no other odorants. This ORN is termed here pb2D. This (pb2D, pb2B) sensillum represents 21% of the sensilla containing pb2A or pb2B neurons in flies expressing acj6^{1,3,4.Scer\UAS}. The second type of sensilla, representing 53% of the pb2 sensilla in acj6^{1,3,4.Scer\UAS} expressing flies, contains a normal pb2B cell and a novel ORN that responds to both 3-octanol and fenchone and is termed her pb2E.

Expression of acj6^{1,3,4.Scer\UAS} under the control of Scer\GAL4^acj6-PG63 in a acj6^PG63/Y background, fully rescues the sensilla pb1 phenotype, yielding (pb1A, pb1B) sensilla that respond as expected to all odorants tested (ethyl acetate, isoamyl acetate, 4-methyl phenol, benzaldehyde, 3-octanol and 4-propyl phenol).

Expression of acj6^{1,3,4.Scer\UAS} under the control of Scer\GAL4^acj6-PG63 in a acj6^PG63/Y background rescues the pb3 sensilla.

(Bai and Carlson, 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

acj6^{1,3,4.Scer\UAS}

acj6^1,3,4.UAS

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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