FlyBase Allele Report: Dmel\slmb[L.hs]

General Information

Symbol

Dmel\slmb^L.hs

Species

D. melanogaster

Name

FlyBase ID

FBal0117353

Feature type

allele

Associated gene

Dmel\slmb

Associated Insertion(s)

Carried in Construct

P{hs-slmb.L}

Also Known As

hs-slmb, HS-slmbL

Key Links

Transgenic product class

cDNA

(Miletich and Limbourg-Bouchon, 2000)

wild_type

(Miletich and Limbourg-Bouchon, 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Miletich and Limbourg-Bouchon, 2000)

wild_type

(Miletich and Limbourg-Bouchon, 2000)

Mutagen

in vitro construct

(Miletich and Limbourg-Bouchon, 2000)

Progenitor genotype

Carried in construct

P{hs-slmb.L}

Cytology

Description

A 3kb fragment of the slmb cDNA, including the entire ORF and a large part of the 3' untranslated region is driven by a heat-shock promoter.

(Miletich and Limbourg-Bouchon, 2000)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

slmb

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm, with slmb⁸

(Grima et al., 2002)

abnormal locomotor rhythm, with slmb⁸

(Grima et al., 2002)

lethal - all die during first instar larval stage, with slmb⁸

(Heriche et al., 2003)

lethal - all die during third instar larval stage | heat sensitive, with slmb⁸

(Heriche et al., 2003)

Phenotype Manifest In

egg chamber, with slmb^9H4-17/slmb⁸

(Morais-de-Sá et al., 2014)

Detailed Description

Statement

Reference

slmb^L.hs; slmb⁸/slmb^9H4-17 transheterozygous ovaries exhibit defects in stau localisation and in the organisation of epithelial follicle cells. Mutant egg chambers exhibit multiple cell layers.

(Morais-de-Sá et al., 2014)

slmb⁸, slmb^L.hs mutants, in the absence of heat shock, exhibit early lethality. A single 30 minute heat shock at 37[o]C during first instar is enough to allow some larvae to reach the wandering third instar stage 4 days later.

(Heriche et al., 2003)

When slmb^L.hs is used to rescue slmb⁸ mutants to adulthood, mutant adults completely arrhythmic in constant dark (DD) conditions. Ventral lateral neurons are normal. The addition of slmb^Scer\UAS.cGa driven by Scer\GAL4^P2.4.Pdf or Scer\GAL4¹¹¹⁸ rescues this phenotype.

(Grima et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

slmb^9H4-17/slmb⁸, slmb^L.hs has egg chamber phenotype, suppressible | partially by aPKC^k06403/aPKC[+]

(Morais-de-Sá et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Lowering aPKC levels, by a aPKC^k06403 heterozygous background, partially suppresses the multilayering cell phenotype found in slmb^L.hs;slmb⁸/slmb^9H4-17 mutants.

(Morais-de-Sá et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

slmb⁸/slmb^L.hs is rescued by slmb^UAS.cGa/Scer\GAL4¹¹¹⁸

(Grima et al., 2002)

slmb⁸/slmb^L.hs is rescued by slmb^UAS.cGa/Scer\GAL4^P2.4.Pdf

(Grima et al., 2002)

Rescues

slmb^L.hs rescues slmb⁸

(Grima et al., 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

slmb^L.hs

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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