FlyBase Allele Report: Dmel\trbl[EP3519]

General Information

Symbol

Dmel\trbl^EP3519

Species

D. melanogaster

Name

FlyBase ID

FBal0117324

Feature type

allele

Associated gene

Dmel\trbl

Associated Insertion(s)

P{EP}trbl^EP3519

Carried in Construct

Also Known As

EP(3)3519

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gene Disruption Project members, 2001-, Abdelilah-Seyfried et al., 2000, Großhans and Wieschaus, 2000, Mata et al., 2000, Rorth et al., 2000, Seher and Leptin, 2000)

Progenitor genotype

Associated Insertion(s)

P{EP}trbl^EP3519

Cytology

Description

P{EP} insertion.

(Abdelilah-Seyfried et al., 2000)

P{EP} insertion in the 5' untranslated region.

(Großhans and Wieschaus, 2000)

P{EP} insertion upstream of trbl. The transcriptional orientation of the P{EP} element is the same as the direction of transcription of the trbl locus.

(Mata et al., 2000)

The P{EP} insertion is 8bp upstream of the presumed transcription start site of trbl.

(Seher and Leptin, 2000)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:20,401,596..20,401,596 [-]

(Otsuki and Brand, 2018, Schulz et al., 2004, Gene Disruption Project members, 2001-, Abdelilah-Seyfried et al., 2000, Großhans and Wieschaus, 2000, Mata et al., 2000, Rorth et al., 2000, Seher and Leptin, 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle

(Seher and Leptin, 2000)

increased occurrence of cell division | embryonic stage

(Seher and Leptin, 2000)

increased occurrence of cell division | spermatogenesis, with Scer\GAL4^{VP16.nanos.UTR}

(Schulz et al., 2004)

partially lethal - majority die (with trbl^D3)

(Masoner et al., 2013)

partially lethal - majority die (with trbl^D13)

(Masoner et al., 2013)

partially lethal - majority live

(Großhans and Wieschaus, 2000)

visible, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

wild-type, with Scer\GAL4^ap-md544

(Seher and Leptin, 2000)

wild-type, with Scer\GAL4^GMR.12

(Seher and Leptin, 2000)

wild-type, with Scer\GAL4^GMR.15

(Seher and Leptin, 2000)

wild-type, with Scer\GAL4^hs.2sev

(Seher and Leptin, 2000)

wild-type, with Scer\GAL4^Kr.PM

(Seher and Leptin, 2000)

wild-type, with Scer\GAL4^prd.RG1

(Seher and Leptin, 2000)

wild-type, with Scer\GAL4^sd-SG29.1

(Seher and Leptin, 2000)

wild-type, with Scer\GAL4^twi.PG

(Seher and Leptin, 2000)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of trbl^EP3519 under the control of Scer\GAL4^slbo.2.6 partially blocks border cell migration.

(Masoner et al., 2013)

Expression of trbl^EP3519 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} (the animals are initially raised at 18^oC and are then shifted to 29^oC after 7 days) causes spermatogonia to continue mitotic proliferation at the expense of differentiation. This defect is seen in at least 50% of testes examined.

(Schulz et al., 2004)

Expression of trbl^EP3519 under the control of Scer\GAL4^sca-537.4 results in the loss of macrochaetae. Sensory organs with no socket and one shaft and thickened shafts are seen.

(Abdelilah-Seyfried et al., 2000)

Cells in the ventral domain (mitotic domain 10) are the first to enter mitosis in trbl^EP3519 embryos. The order of the other mitotic domains is not altered. As a consequence of the early mitosis in mitotic domain 10, the mesodermal precursors remain on the surface and do not form a proper ventral furrow.

(Großhans and Wieschaus, 2000)

Expression of trbl^EP3519 under the control of Scer\GAL4^slbo.2.6 affects border cell migration.

(Rorth et al., 2000)

At the time when the ventral furrow has invaginated fully in wild-type, in homozygous trbl^EP3519 mutant embryos, the majority of the prospective mesodermal cells are in mitosis (dividing too early) and no furrow is formed. In contrast to wild-type homozygous mutants approximately 75% of embryos have dividing pole cells between stage 6 and stage 10. Only individual cells are seen in mitosis. No phenotype is seen when trbl^EP3519 is driven by Scer\GAL4^twi.PG, Scer\GAL4^prd.RG1, Scer\GAL4^Kr.PM, Scer\GAL4^sd-SG29.1, Scer\GAL4^ap-md544, Scer\GAL4^hs.2sev, Scer\GAL4^GMR.15 or Scer\GAL4^GMR.12.

(Seher and Leptin, 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^sca-537.4, trbl^EP3519 has visible phenotype, enhanceable by N^55e11

(Abdelilah-Seyfried et al., 2000)

Scer\GAL4^sca-537.4, trbl^EP3519 has visible phenotype, enhanceable by H^E21

(Abdelilah-Seyfried et al., 2000)

Suppressor of

Statement

Reference

trbl^EP3519/Scer\GAL4^vg.PM is a suppressor of visible phenotype of Scer\GAL4^vg.PM, slbo^UAS.cRa

(Rorth et al., 2000)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^sca-537.4/trbl^EP3519 is an enhancer of phenotype of N^55e11

(Abdelilah-Seyfried et al., 2000)

Scer\GAL4^sca-537.4/trbl^EP3519 is an enhancer of phenotype of H^E21

(Abdelilah-Seyfried et al., 2000)

Scer\GAL4^sca-537.4/trbl^EP3519 is an enhancer of external sensory organ phenotype of H^E21

(Abdelilah-Seyfried et al., 2000)

Suppressor of

Statement

Reference

Scer\GAL4^sca-537.4/trbl^EP3519 is a suppressor of phenotype of H^E21

(Abdelilah-Seyfried et al., 2000)

Scer\GAL4^sca-537.4/trbl^EP3519 is a suppressor of trichogen cell phenotype of H^E21

(Abdelilah-Seyfried et al., 2000)

trbl^EP3519/Scer\GAL4^slbo.2.6 is a suppressor of border follicle cell phenotype of Scer\GAL4^slbo.2.6, slbo^UAS.cRa

(Rorth et al., 2000)

trbl^EP3519/Scer\GAL4^vg.PM is a suppressor of wing phenotype of Scer\GAL4^vg.PM, slbo^UAS.cRa

(Rorth et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The small wing phenotype caused by expression of slbo^Scer\UAS.cRa under the control of Scer\GAL4^vg.PM is suppressed by coexpression of trbl^EP3519. The delay in border cell migration caused by expression of slbo^Scer\UAS.cRa under the control of Scer\GAL4^slbo.2.6 is suppressed by coexpression of trbl^EP3519.

(Rorth et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

CG33969^EY20583

(Masoner et al., 2013)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

58441

w¹¹¹⁸; P{EP}trbl^EP3519/TM3, Sb¹

Notes on Origin

Discoverer

Comments

Precise excision of the P{EP} element reverts the mutant phenotype to full viability and normal gastrulation.

(Großhans and Wieschaus, 2000)

Mutant phenotype can be reverted by excising the P{EP} insertion.

(Seher and Leptin, 2000)

Order of allelic strength: trbl^D13 = trbl^D3 > trbl^EP3519 > trbl^EP1119.

(Masoner et al., 2013)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

EP(3)3519

(Abdelilah-Seyfried et al., 2000, Großhans and Wieschaus, 2000, Seher and Leptin, 2000)

trbl³⁵¹⁹

(LaFerriere and Zars, 2017)

trbl^EP3519

(Otsuki and Brand, 2018, Masoner et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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