FB2024_03 , released June 25, 2024
Allele: Dmel\Cdk7T170A.UAS.Tag:HA
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General Information
Symbol
Dmel\Cdk7T170A.UAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0105264
Feature type
allele
Associated gene
Dmel\Cdk7
Associated Insertion(s)
Carried in Construct
P{UAS-Cdk7.T170A}
Key Links
Transgenic product class
missense_variant
(Leclerc et al., 2000)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Leclerc et al., 2000)
Mutagen
in vitro construct
(Leclerc et al., 2000)
Progenitor genotype
Carried in construct
P{UAS-Cdk7.T170A}
Cytology
Description

UAS sequences drive a mutated Cdk7 cDNA (carries the amino acid replacement D137A) tagged at the C terminus with Tag:HA.

(Leclerc et al., 2000)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Cdk7
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      When Cdk7T170A.Scer\UAS.T:Ivir\HA1 is driven by Scer\GAL4Bx-MS1096 malformations are induced in the wing. They range from alterations of the vein pattern to severe wing blade reduction. This phenotype is enhanced by the addition of Cdk7null3. When Cdk7T170A.Scer\UAS.T:Ivir\HA1 is expressed in early embryos under the control of Scer\GAL4Kr.PM, at the end of the maternal division program, a corresponding zone with twice the normal density of interphase 14 nuclei are observed, resulting from the appearance of an extra synchronous division in the domain of expression of the Cdk7T170A.Scer\UAS.T:Ivir\HA1 proteins.

      (Leclerc et al., 2000)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Extra copies of twe+t3.7 or twe+t10 or combinations thereof, enhance the mitotic cycle phenotype seen in Cdk7T170A.Scer\UAS.T:Ivir\HA1 flies, when driven by Scer\GAL4Kr.PM, in a dose dependant manner.

      (Leclerc et al., 2000)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Cdk7T170A.UAS.Tag:HA is rescued by Scer\GAL4Bx-MS1096/Cdk7UAS.Tag:HA

      (Leclerc et al., 2000)
      Fails to rescue

      Cdk7T170A.UAS.Tag:HA/Scer\GAL4Bx-MS1096 fails to rescue Cdk7null3

      (Leclerc et al., 2000)
      Comments

      Expression of Cdk7T170A.Scer\UAS.T:Ivir\HA1 by Scer\GAL4Bx-MS1096 or under basal expression under the control of the Hsp70 promoter fails to rescue the Cdk7null3 wing phenotypes.

      (Leclerc et al., 2000)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Cdk7DN
      (Leclerc et al., 2000)
      Cdk7T170A.Scer\UAS.T:Ivir\HA1
      Cdk7T170A.UAS.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (1)