Construct: Amino acid replacement: L1P. Amino acid replacement: Y12F. Amino acid replacement: T23F. Amino acid replacement: M35I. Amino acid replacement: I61L. Amino acid replacement: Q62R. Amino acid replacement: I64V. Amino acid replacement: L67I. All of the amino acids in the homeodomain and C terminus conserved region are changed to the equivalent residue in abd-A. The numbering is from the first amino acid in the homeodomain. Expression of this mutated Ubx1a is driven by Hsp70 promoter.
Ubiquitous expression of UbxA.hs transforms the three thoracic segments and the first abdominal segment into extra A2 segments. This transformation is less complete for segments T2 and T3.