FlyBase Allele Report: Dmel\ci[UAS.cDa]

General Information

Symbol

Dmel\ci^UAS.cDa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Dahmann

FlyBase ID

FBal0104669

Feature type

allele

Associated gene

Dmel\ci

Associated Insertion(s)

Carried in Construct

P{UAS-ci.D}

Also Known As

UAS-ci

Key Links

Transgenic product class

wild_type

(Dahmann and Basler, 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Dahmann and Basler, 2000)

Mutagen

in vitro construct

(Dahmann and Basler, 2000)

Progenitor genotype

Carried in construct

P{UAS-ci.D}

Cytology

Description

UASt regulatory sequences drive expression of the full length coding region of ci.

(Dahmann and Basler, 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased occurrence of cell division | larval stage | somatic clone, with Scer\GAL4^Act5C.PP

(Duman-Scheel et al., 2002)

Phenotype Manifest In

Detailed Description

Statement

Reference

Clones of ci^Scer\UAS.cDa in which expression is driven by Scer\GAL4^hh-Gal4, generated along the A/P boundary of the wing disc using the MARCM technique, are misplaced into the A territory.

(Shen and Dahmann, 2005)

Cells in clones in the morphogenetic furrow in the eye disc expressing ci^Scer\UAS.cDa under the control of Scer\GAL4^Act5C.PP enter S phase (they are normally arrested in G1). Expression of ci^Scer\UAS.cDa under the control of Scer\GAL4^C96 promotes S phase in G1-arrested cells in the wing margin in wing discs. Clones in the wing disc expressing ci^Scer\UAS.cDa under the control of Scer\GAL4^Act5C.PP are significantly larger than control clones, covering 143% of the area covered by control clones. Cell proliferation rates in the clone are accelerated, but individual cell size and overall cell cycle phasing are unaffected.

(Duman-Scheel et al., 2002)

Cells in the far anterior compartment of the wing imaginal disc expressing ci^Scer\UAS.cDa under the control of Scer\GAL4^Act5C.PP have wiggly borders to their neighbours. In contrast, cells in the posterior compartment of the wing imaginal disc expressing ci^Scer\UAS.cDa under the control of Scer\GAL4^Act5C.PP sort out from wild-type posterior cells. Clones of posterior origin in the wing disc which are expressing ci^Scer\UAS.cDa under the control of Scer\GAL4^Act5C.PP take up positions normally occupied only by anterior cells and define straight borders to wild-type posterior cells. Anterior clones are strictly confined to anterior territory.

(Dahmann and Basler, 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^Act5C.PP, ci^UAS.cDa has increased occurrence of cell division | somatic clone | larval stage phenotype, suppressible by dap^UAS.cLa/Scer\GAL4^Act5C.PP

(Duman-Scheel et al., 2002)

NOT suppressed by

Statement

Reference

Scer\GAL4^Act5C.PP, ci^UAS.cDa has increased occurrence of cell division | somatic clone | larval stage phenotype, non-suppressible by Rbf^280.UAS, Scer\GAL4^Act5C.PP

(Duman-Scheel et al., 2002)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^Act5C.PP, ci^UAS.cDa has S phase | ectopic | somatic clone phenotype, suppressible by dap^UAS.cLa/Scer\GAL4^Act5C.PP

(Duman-Scheel et al., 2002)

Scer\GAL4^Act5C.PP, ci^UAS.cDa has eye disc morphogenetic furrow | somatic clone phenotype, suppressible by dap^UAS.cLa/Scer\GAL4^Act5C.PP

(Duman-Scheel et al., 2002)

NOT suppressed by

Statement

Reference

Scer\GAL4^Act5C.PP, ci^UAS.cDa has S phase | ectopic phenotype, non-suppressible by Scer\GAL4^bbg-C96/Rbf^280.UAS

(Duman-Scheel et al., 2002)

Scer\GAL4^Act5C.PP, ci^UAS.cDa has wing disc phenotype, non-suppressible by Scer\GAL4^bbg-C96/Rbf^280.UAS

(Duman-Scheel et al., 2002)

Scer\GAL4^Act5C.PP, ci^UAS.cDa has S phase | ectopic | somatic clone phenotype, non-suppressible by Rbf^280.UAS, Scer\GAL4^Act5C.PP

(Duman-Scheel et al., 2002)

Scer\GAL4^Act5C.PP, ci^UAS.cDa has eye disc morphogenetic furrow | somatic clone phenotype, non-suppressible by Rbf^280.UAS, Scer\GAL4^Act5C.PP

(Duman-Scheel et al., 2002)

Suppressor of

Statement

Reference

ci^UAS.cDa/Scer\GAL4^hh-Gal4 is a suppressor of anterior-posterior compartment boundary of the wing disc | somatic clone phenotype of bi^omb-3198

(Shen and Dahmann, 2005)

ci^UAS.cDa/Scer\GAL4^hh-Gal4 is a suppressor of wing disc anterior compartment | somatic clone phenotype of bi^omb-3198

(Shen and Dahmann, 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The bi^omb-3198 mutation prevents the misplacing of ci^Scer\UAS.cDa, Scer\GAL4^hh-Gal4 clones that are generated along the A/P boundary into the A territory.

(Shen and Dahmann, 2005)

Co-expression of Rbf^280.Scer\UAS does not block the ability of ci^Scer\UAS.cDa (expressed under the control of Scer\GAL4^Act5C.PP) to induce S phase in clones in the morphogenetic furrow. Co-expression of Rbf^280.Scer\UAS does not block the ability of ci^Scer\UAS.cDa (expressed under the control of Scer\GAL4^C96) to induce S phase in cells in the wing margin. Co-expression of dap^Scer\UAS.cLa blocks the ability of ci^Scer\UAS.cDa (expressed under the control of Scer\GAL4^Act5C.PP) to induce S phase in clones in the morphogenetic furrow. Expression of ci^Scer\UAS.cDa under the control of Scer\GAL4^Act5C.PP does not significantly increase the size of clones induced in the wing disc in a Cdk4³ mutant background.

(Duman-Scheel et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ci^Scer\UAS.cDa

ci^UAS.cDa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Dahmann

(Dahmann and Basler, 2000)

Secondary FlyBase IDs

References (5)

Report Sections

Open Close