FlyBase Allele Report: Dmel\Pten[UAS.cGa]

General Information

Symbol

Dmel\Pten^UAS.cGa

Species

D. melanogaster

Name

FlyBase ID

FBal0104062

Feature type

allele

Associated gene

Dmel\Pten

Associated Insertion(s)

Carried in Construct

P{UAS-Pten.G}

Also Known As

UAS-dPTEN

Key Links

Transgenic product class

wild_type

(von Stein et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(von Stein et al., 2005)

Mutagen

in vitro construct

(von Stein et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-Pten.G}

Cytology

Description

UAS regulatory sequences drive expression of Pten isoform 3.

(von Stein et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Pten

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number, with Scer\GAL4^dpp.blk1

(Goberdhan et al., 1999)

decreased cell size, with Scer\GAL4^dpp.blk1

(Goberdhan et al., 1999)

partially lethal - majority die, with Scer\GAL4^en-e16E

(Goberdhan et al., 1999)

visible, with Scer\GAL4^ap-md544

(Lee et al., 2010)

visible, with Scer\GAL4^ci.PU

(Mesquita et al., 2010)

visible, with Scer\GAL4^salm.PU

(Mesquita et al., 2010)

Phenotype Manifest In

embryo | dorsal closure stage, with Scer\GAL4^en.PU

(Pickering et al., 2013)

embryo | dorsal closure stage, with Scer\GAL4^αTub84B.PL

(Pickering et al., 2013)

embryonic leading edge cell, with Scer\GAL4^en.PU

(Pickering et al., 2013)

wing, with Scer\GAL4^ci.PU

(Mesquita et al., 2010)

wing, with Scer\GAL4^dpp.blk1

(Goberdhan et al., 1999)

wing, with Scer\GAL4^salm.PU

(Mesquita et al., 2010)

wing blade, with Scer\GAL4^ap-md544

(Lee et al., 2010)

wing blade posterior compartment, with Scer\GAL4^en-e16E

(Goberdhan et al., 1999)

Detailed Description

Statement

Reference

The dorsal hole is elongated compared to wild type during dorsal closure in embryos expressing Pten^Scer\UAS.cGa under the control of Scer\GAL4^αTub84B.PL.

Cells expressing Pten^Scer\UAS.cGa under the control of Scer\GAL4^en.PU show a significant decrease in zippering speed during dorsal closure compared to neighbouring control cells. This results in the mutant cells often zippering later than more central wild-type cells, leaving transient gaps in the zippering seam. The number of filopodia at the leading edge is significantly reduced in the mutant cells.

(Pickering et al., 2013)

Expression of Pten^Scer\UAS.cGa driven by Scer\GAL4^ap-md544 in dorsal cells of the wing causes a phenotype in which the wing bends upward.

(Lee et al., 2010)

Expression of Thor^{T37A.T46A.Scer\UAS} under the control of Scer\GAL4^ci.PU results in a reduction in total wing area compared to control flies. A reduction in area of both the wing compartment expressing the Thor^{T37A.T46A.Scer\UAS} transgene and the neighbouring, non-expressing wing compartment is seen.

Expression of Pten^Scer\UAS.cGa under the control of Scer\GAL4^salm.PU results in wings with smaller wings than normal, without a large impact of the overall size of the animal (adult weight and pupal length are unchanged compared to controls).

A clear increase in the number of TUNEL-positive cells is seen in the territory of Pten^Scer\UAS.cGa expression in wing discs expressing Pten^Scer\UAS.cGa under the control of Scer\GAL4^en-e16E, as well as in adjacent cells that are not expressing Pten^Scer\UAS.cGa.

(Mesquita et al., 2010)

Hemocytes in Pten^Scer\UAS.cGa; Scer\GAL4^srp.Hemo late embryos do not exhibit abnormal aggregation.

(Bruckner et al., 2004)

When expression is driven by Scer\GAL4^dpp.blk1 the area between wing veins LIII and LIV is significantly reduced due to a decrease in both cell number and cell size.

When expression is driven by Scer\GAL4^en-e16E, viability is decreased, but in the 20% of flies that eclose from pupae the posterior compartment is reduced in size.

(Goberdhan et al., 1999)

When expression is driven by Scer\GAL4^dpp.blk1 the area between wing veins LIII and LIV is significantly reduced due to a decrease in both cell number and cell size. When expression is driven by Scer\GAL4^en-e16E, viability is decreased, but in the 20% of flies that eclose from pupae the posterior compartment is reduced in size.

(Goberdhan et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Pten^UAS.cGa, Scer\GAL4^ap-md544 has visible phenotype, enhanceable by Sesn^d04539, Scer\GAL4^ap-md544

(Lee et al., 2010)

Suppressed by

Statement

Reference

Pten^UAS.cGa, Scer\GAL4^en-e16E has partially lethal - majority die phenotype, suppressible | partially by Pi3K92E^UAS.Tag:MYC, Scer\GAL4^en-e16E

(Goberdhan et al., 1999)

Suppressor of

Statement

Reference

Pten^UAS.cGa, Scer\GAL4^en-e16E is a suppressor | partially of partially lethal - majority die phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^en-e16E

(Goberdhan et al., 1999)

Pten^UAS.cGa, Scer\GAL4^en-e16E is a suppressor | partially of some die during pupal stage phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^en-e16E

(Goberdhan et al., 1999)

Pten^UAS.cGa, Scer\GAL4^en-e16E is a suppressor | partially of some die during larval stage phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^en-e16E

(Goberdhan et al., 1999)

Other

Statement

Reference

Dcp-1^UAS.cKa, Pten^UAS.cGa, Scer\GAL4^GMR.PF has lethal phenotype

(Kim et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Pten^UAS.cGa, Scer\GAL4^ap-md544 has wing blade phenotype, enhanceable by Sesn^d04539, Scer\GAL4^ap-md544

(Lee et al., 2010)

Suppressed by

Statement

Reference

Pten^UAS.cGa, Scer\GAL4^en.PU has embryo | dorsal closure stage phenotype, suppressible by Rac1^UAS.cLa, Scer\GAL4^en.PU

(Pickering et al., 2013)

Pten^UAS.cGa, Scer\GAL4^en.PU has embryonic leading edge cell phenotype, suppressible by Rac1^UAS.cLa, Scer\GAL4^en.PU

(Pickering et al., 2013)

Pten^UAS.cGa, Scer\GAL4^en-e16E has wing | posterior phenotype, suppressible by Pi3K92E^UAS.Tag:MYC, Scer\GAL4^en-e16E

(Goberdhan et al., 1999)

Suppressor of

Statement

Reference

Pten^UAS.cGa, Scer\GAL4^en-e16E is a suppressor of wing | posterior phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^en-e16E

(Goberdhan et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Rac1^Scer\UAS.cLa suppresses the decrease in zippering speed during dorsal closure seen in cells expressing Pten^Scer\UAS.cGa under the control of Scer\GAL4^en.PU. The reduction in number of filopodia at the leading edge is also suppressed.

(Pickering et al., 2013)

Co-expression of Pten^Scer\UAS.cGa and Dcp-1^Scer\UAS.cKa (insertion line P{UAS-Dcp-1.K}19-2) under the control of Scer\GAL4^GMR.PF is lethal.

(Kim et al., 2010)

Co-expression of Sesn^d04539 with Pten^Scer\UAS.cGa driven by Scer\GAL4^ap-md544 enhances the wing-bending phenotype of Pten^Scer\UAS.cGa.

(Lee et al., 2010)

Flies expressing both Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX} and Pten^Scer\UAS.cGa driven by Scer\GAL4^en-e16E show a much higher viability than those expressing either alone. The posterior compartment of the wing is nearly as reduced in area as in animals expressing Pten^Scer\UAS.cGa driven by Scer\GAL4^en-e16E alone.

(Goberdhan et al., 1999)

Flies expressing both Pi3K92E^{Scer\UAS.T:Hsap\MYC} and Pten^Scer\UAS.cGa driven by Scer\GAL4^en-e16E show a much higher viability than those expressing either alone. The posterior compartment of the wing is nearly as reduced in area as in animals expressing Pten^Scer\UAS.cGa driven by Scer\GAL4^en-e16E alone.

(Goberdhan et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Pten^Scer\UAS.cGa

Pten^UAS.cGa

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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