FlyBase Allele Report: Dmel\Src42A[CA.UAS]

General Information

Symbol

Dmel\Src42A^CA.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0103957

Feature type

allele

Associated gene

Dmel\Src42A

Associated Insertion(s)

Carried in Construct

P{UAS-Src42A.CA}

Also Known As

UAS-Src42A^CA, UAS-Src42A.CA, UAS-Src42^CA, Src42A^CA, UAS-Src42A^YF, UAS-Src42A-CA

Key Links

Transgenic product class

missense_variant

(Tateno et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Tateno et al., 2000)

Mutagen

in vitro construct

(Tateno et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-Src42A.CA}

Cytology

Description

UASt regulatory sequences drive expression of a constitutively active form of Src42A (carries the amino acid replacement Y511F), with the mutation described above.

(Tateno et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Src42A

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of colorectal cancer

CEA

(Cordero et al., 2014)

model of intestinal cancer

CEA

(Kohlmaier et al., 2015)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of colorectal cancer

is ameliorated by Egfr^KK100051

(Cordero et al., 2014)

is ameliorated by Stat92E^GD4492

(Cordero et al., 2014)

is ameliorated by Egfr^t1

(Cordero et al., 2014)

is ameliorated by Ras85D^KK108029

(Cordero et al., 2014)

is ameliorated by Ras85D^GD12553

(Cordero et al., 2014)

is NOT ameliorated by dome^GD2612

(Cordero et al., 2014)

model of intestinal cancer

is ameliorated by Egfr^RNAi.UAS.cUa

(Kohlmaier et al., 2015)

is ameliorated by Stat92E^RNAi.UAS.cUa

(Kohlmaier et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell adhesion | wandering third instar larval stage, with Scer\GAL4^GMR.PF

(Pütz, 2019)

abnormal mitotic cell cycle | adult stage, with Scer\GAL4^NP7397

(Kohlmaier et al., 2015)

abnormal neuroanatomy | wandering third instar larval stage, with Scer\GAL4^GMR.PF

(Pütz, 2019)

abnormal planar polarity | embryonic stage | maternal effect, with Scer\GAL4^{VP16.mat.αTub67C}

(Tamada et al., 2021)

abnormal size | wandering third instar larval stage, with Scer\GAL4^GMR.PF

(Pütz, 2019)

hyperplasia, with Scer\GAL4^NP5130

(Cordero et al., 2014)

increased cell number | adult stage, with Scer\GAL4^NP7397

(Kohlmaier et al., 2015)

increased occurrence of cell division | adult stage, with Scer\GAL4^NP5130

(Cordero et al., 2014)

increased occurrence of cell division | adult stage, with Scer\GAL4^NP7397

(Perea et al., 2017, Kohlmaier et al., 2015)

lethal, with Scer\GAL4^hs.2sev

(Pütz, 2019)

majority die during larval stage, with Scer\GAL4^hs.2sev

(Pütz, 2019)

Phenotype Manifest In

actin filament | ectopic, with Scer\GAL4^slbo.2.6

(Somogyi and Rorth, 2004)

adherens junction | wandering third instar larval stage, with Scer\GAL4^GMR.PF

(Pütz, 2019)

adult midgut, with Scer\GAL4^NP7397

(Kohlmaier et al., 2015)

border follicle cell, with Scer\GAL4^slbo.2.6

(Somogyi and Rorth, 2004)

embryo | maternal effect, with Scer\GAL4^{VP16.nanos.UTR}

(Hayden et al., 2022)

embryonic/larval tracheal dorsal trunk | embryonic stage 17, with Scer\GAL4^btl.PS

(Öztürk-Çolak et al., 2016)

embryonic/larval tracheal section, with Scer\GAL4^btl.PS

(Förster and Luschnig, 2012, Shindo et al., 2008)

eye disc | wandering third instar larval stage, with Scer\GAL4^GMR.PF

(Pütz, 2019)

intestinal stem cell of posterior adult midgut epithelium, with Scer\GAL4^NP5130

(Cordero et al., 2014)

intestinal stem cell of posterior adult midgut epithelium, with Scer\GAL4^NP7397

(Perea et al., 2017)

photoreceptor R3 precursor cell | wandering third instar larval stage, with Scer\GAL4^GMR.PF

(Pütz, 2019)

photoreceptor R4 precursor cell | wandering third instar larval stage, with Scer\GAL4^GMR.PF

(Pütz, 2019)

taenidial fold | embryonic stage 17, with Scer\GAL4^btl.PS

(Öztürk-Çolak et al., 2016)

Detailed Description

Statement

Reference

Expressing Src42A^CA.UAS under the control of Scer\GAL4^GMR.PF leads to massive defects in eye development when flies are raised at 25[o]C, but only mild defects at 18[o]C. At 18[o]C a significant proportion of CadN-immunopositive adherens junctions between photoreceptor precursor cells R3 and R4 in row 5 ommatidia of wandering third instar larvae are shorter compared to controls.

(Pütz, 2019)

Adult flies expressing Src42A^CA.Scer\UAS driven by Scer\GAL4^esg-NP7397 combined with tub-Gal80[ts] (for temporal control) display significant increase in midgut stem cell proliferation (assessed by number of pH3-positive cells).

(Perea et al., 2017)

In the tracheal dorsal trunk of stage 17 embryos, expression of Src42A^CA.UAS under the control of Scer\GAL4^btl.PS leads to disruption of taenidial fold structure and F-actin bundling when compared to controls.

(Öztürk-Çolak et al., 2016)

Scer\GAL4^esg-NP5130-mediated expression of Src64B^Scer\UAS.cCa (restricted to adult stages by using P{tubP-GAL80^ts}) results in substantial intestinal stem cell hyperproliferation and hyperplasia of the adult intestine.

(Cordero et al., 2014)

Expression of Src42A^Scer\UAS.cPa under the control of Scer\GAL4^btl.PS results in over-elongated and convoluted dorsal trunks. Dorsal trunk metameres are 23% longer than in controls although dorsal trunk cell number is unchanged. Cells are flattened and markedly elongated axially.

(Förster and Luschnig, 2012)

Expression of Src42A^CA.Scer\UAS under the control of Scer\GAL4^btl.PS causes disintegration of the tracheal epithelium. Tracheal cell contacts persist but becomes loose, cells become rounded and the lumen becomes discontinuous. Dorsal branch tip cells occasionally become detached, and in some cases tracheal cells that are detached from one branch re-attach to a neighbouring branch. Tracheal cells fail to stabilise cell junctions and to extend dorsal branches, although they retain filopodia activity.

(Shindo et al., 2008)

When Src42A^CA.Scer\UAS is driven by Scer\GAL4^slbo.2.6 in the follicle cells, an increase is seen in the accumulation of F-actin. These cells also show a disruption of the normal cell shape. Border cell migration is completely blocked.

(Somogyi and Rorth, 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^NP7397, Src42A^CA.UAS has increased occurrence of cell division | adult stage phenotype, non-enhanceable by Scer\GAL4^NP7397/dome^ΔTMCYT.UAS

(Kohlmaier et al., 2015)

Suppressed by

Statement

Reference

Scer\GAL4^NP7397, Src42A^CA.UAS has increased occurrence of cell division | adult stage phenotype, suppressible by Ret^GD45, Scer\GAL4^NP7397

(Perea et al., 2017)

Scer\GAL4^NP7397, Src42A^CA.UAS has increased occurrence of cell division | adult stage phenotype, suppressible by Scer\GAL4^NP7397/Stat92E^RNAi.UAS.cUa

(Kohlmaier et al., 2015)

Scer\GAL4^NP5130, Src42A^CA.UAS has hyperplasia phenotype, suppressible by Ras85D^KK108029, Scer\GAL4^NP5130

(Cordero et al., 2014)

Scer\GAL4^NP5130, Src42A^CA.UAS has hyperplasia phenotype, suppressible by Ras85D^GD12553, Scer\GAL4^NP5130

(Cordero et al., 2014)

Scer\GAL4^NP5130, Src42A^CA.UAS has hyperplasia phenotype, suppressible by Egfr^KK100051, Scer\GAL4^NP5130

(Cordero et al., 2014)

Scer\GAL4^NP5130, Src42A^CA.UAS has hyperplasia phenotype, suppressible by Egfr[+]/Egfr^t1

(Cordero et al., 2014)

Scer\GAL4^NP5130, Src42A^CA.UAS has hyperplasia phenotype, suppressible by Stat92E^GD4492/Scer\GAL4^NP5130

(Cordero et al., 2014)

Scer\GAL4^NP5130, Src42A^CA.UAS has increased occurrence of cell division | adult stage phenotype, suppressible by Hsap\MATK^UAS.cVa, Scer\GAL4^NP5130

(Cordero et al., 2014)

NOT suppressed by

Statement

Reference

Scer\GAL4^NP7397, Src42A^CA.UAS has increased occurrence of cell division | adult stage phenotype, non-suppressible by Scer\GAL4^NP7397/dome^ΔTMCYT.UAS

(Kohlmaier et al., 2015)

Scer\GAL4^NP5130, Src42A^CA.UAS has hyperplasia phenotype, non-suppressible by dome^GD2612/Scer\GAL4^NP5130

(Cordero et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^btl.PS, Src42A^CA.UAS has embryonic/larval tracheal section phenotype, enhanceable by shg[+]/shg²

(Shindo et al., 2008)

Scer\GAL4^btl.PS, Src42A^CA.UAS has embryonic/larval tracheal section phenotype, enhanceable by shg²

(Shindo et al., 2008)

Scer\GAL4^btl.PS, Src42A^CA.UAS has embryonic/larval tracheal section phenotype, enhanceable by pan^ΔN.UAS/Scer\GAL4^btl.PS

(Shindo et al., 2008)

NOT Enhanced by

Statement

Reference

Scer\GAL4^NP7397, Src42A^CA.UAS has adult midgut phenotype, non-enhanceable by Scer\GAL4^NP7397/dome^ΔTMCYT.UAS

(Kohlmaier et al., 2015)

Suppressed by

Statement

Reference

Scer\GAL4^NP7397, Src42A^CA.UAS has intestinal stem cell of posterior adult midgut epithelium phenotype, suppressible by Ret^GD45, Scer\GAL4^NP7397

(Perea et al., 2017)

Scer\GAL4^NP7397, Src42A^CA.UAS has adult midgut phenotype, suppressible by Scer\GAL4^NP7397/Stat92E^RNAi.UAS.cUa

(Kohlmaier et al., 2015)

Scer\GAL4^NP5130, Src42A^CA.UAS has intestinal stem cell of posterior adult midgut epithelium phenotype, suppressible by Ras85D^GD12553, Scer\GAL4^NP5130

(Cordero et al., 2014)

Scer\GAL4^NP5130, Src42A^CA.UAS has intestinal stem cell of posterior adult midgut epithelium phenotype, suppressible by Egfr^KK100051, Scer\GAL4^NP5130

(Cordero et al., 2014)

Scer\GAL4^NP5130, Src42A^CA.UAS has intestinal stem cell of posterior adult midgut epithelium phenotype, suppressible by Egfr[+]/Egfr^t1

(Cordero et al., 2014)

Scer\GAL4^NP5130, Src42A^CA.UAS has intestinal stem cell of posterior adult midgut epithelium phenotype, suppressible by Stat92E^GD4492/Scer\GAL4^NP5130

(Cordero et al., 2014)

Scer\GAL4^NP5130, Src42A^CA.UAS has intestinal stem cell of posterior adult midgut epithelium phenotype, suppressible by Ras85D^KK108029, Scer\GAL4^NP5130

(Cordero et al., 2014)

Scer\GAL4^btl.PS, Src42A^CA.UAS has embryonic/larval tracheal section phenotype, suppressible by shg^UAS.sgGFP/Scer\GAL4^btl.PS

(Shindo et al., 2008)

Scer\GAL4^slbo.2.6, Src42A^CA.UAS has actin filament | ectopic phenotype, suppressible | partially by Cortactin^unspecified

(Somogyi and Rorth, 2004)

NOT suppressed by

Statement

Reference

Scer\GAL4^NP7397, Src42A^CA.UAS has adult midgut phenotype, non-suppressible by Scer\GAL4^NP7397/dome^ΔTMCYT.UAS

(Kohlmaier et al., 2015)

Scer\GAL4^NP5130, Src42A^CA.UAS has intestinal stem cell of posterior adult midgut epithelium phenotype, non-suppressible by dome^GD2612/Scer\GAL4^NP5130

(Cordero et al., 2014)

Suppressor of

Statement

Reference

Scer\GAL4^c415/Src42A^CA.UAS is a suppressor of dorsal appendage phenotype of bwk¹⁵¹, cic⁰⁸⁴⁸²

(Tran and Berg, 2003)

NOT Suppressor of

Statement

Reference

Scer\GAL4^unspecified/Src42A^CA.UAS is a non-suppressor of embryonic/first instar larval cuticle | dorsal | germline clone phenotype of Dok^PG155

(Biswas et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of intestinal progenitor cells in mitosis characteristic for adult flies expressing Src42A^CA.Scer\UAS driven by Scer\GAL4^esg-NP7397 (combined with tub-Gal80[ts] for temporal control) is suppressed by co-expression of Ret^GD45.

(Perea et al., 2017)

One copy of shg² enhances the tracheal epithelium defects seen when Src42A^CA.Scer\UAS is expressed under the control of Scer\GAL4^btl.PS. The dorsal trunk is broken in many places and cells are more rounded. Expressing Src42A^CA.Scer\UAS in a homozygous shg² background results in complete cell dissociation.

Expression of shg^{Scer\UAS.T:Avic\GFP-rs} partially suppresses the tracheal defects seen when Src42A^CA.Scer\UAS is expressed under the control of Scer\GAL4^btl.PS. Tracheal cells maintain apico-basal polarity for at least two hours longer than when Src42A^CA.Scer\UAS is expressed alone, and dorsal branch extension is also partially restored.

Expression of pan^ΔN.Scer\UAS enhances the tracheal phenotype seen when Src42A^CA.Scer\UAS is expressed under the control of Scer\GAL4^btl.PS. Tracheal cells lose polarity and dissociate from the epithelium.

(Shindo et al., 2008)

Expression of either the activated Src42A^CA.Scer\UAS transgene fails to rescue Dok^PG155 germ-line clone embryos.

(Biswas et al., 2006)

When Src42A^CA.Scer\UAS is driven by Scer\GAL4^slbo.2.6 in a homozygous Cortactin^unspecified background, a small but significant suppression is seen in the F-Actin phenotype.

(Somogyi and Rorth, 2004)

The mutant dorsal appendage phenotype seen in bwk¹⁵¹/bwk⁰⁸⁴⁸² eggs is suppressed by expression of Src42A^CA.Scer\UAS under the control of Scer\GAL4^c415.

(Tran and Berg, 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

6410

w¹¹¹⁸; P{UAS-Src42A.CA}5

Kyoto

108774

w¹¹¹⁸; P{UAS-Src42A.CA}5

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Src42^Y511F

(Tamada et al., 2021)

Src42A^CA.Scer\UAS

Src42A^CA.UAS

Src42A^CA

(Tsarouhas et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (29)

Report Sections

Open Close