FlyBase Allele Report: Hsap\OTX2[hs.PN]

General Information

Symbol

Hsap\OTX2^hs.PN

Species

H. sapiens

Name

heat shock construct of Nagao

FlyBase ID

FBal0089633

Feature type

allele

Associated gene

Hsap\OTX2

Associated Insertion(s)

Carried in Construct

P{hs-Hsap\OTX2.N}

Key Links

Transgenic product class

wild_type

(Nagao et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Nagao et al., 1998)

Mutagen

in vitro construct

(Leuzinger et al., 1998, Nagao et al., 1998)

Progenitor genotype

Carried in construct

P{hs-Hsap\OTX2.N}

Cytology

Description

An Hsp70 promoter drives Hsap\OTX2 expression.

(Nagao et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Hsap\OTX2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic brain

(Leuzinger et al., 1998)

head & ganglion & embryo

(Leuzinger et al., 1998)

larval ventral nerve cord | embryonic stage

(Leuzinger et al., 1998)

presumptive embryonic/larval central nervous system

(Leuzinger et al., 1998)

presumptive embryonic/larval central nervous system | posterior

(Adachi et al., 2001)

presumptive embryonic/larval peripheral nervous system | posterior

(Adachi et al., 2001)

Detailed Description

Statement

Reference

Overexpression of Hsap\OTX2^hs.PN for 20 minutes at stage 7-8 results in moderate disruption of the central and peripheral nervous systems in the trunk whereas the anterior part of the brain is largely unaffected.

(Adachi et al., 2001)

Overexpression of Hsap\OTX2^hs.PN in a wild-type background at stage 7-8 by a continuous heat shock of over 20 minutes at 37^oC results in the production of transformed or ectopic central nervous system structures in approximately 10% of embryos. These include neuralised embryos, dramatically increased brain lobes and ectopic head ganglionic structures, as well as fused and enlarged ventral ganglia. These transformations are much less pronounced if the heat shock is carried out at stage 9-10, and are not seen if the heat shock is carried out later than stage 12.

(Leuzinger et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Hsap\OTX2^hs.PN is a suppressor of visible | recessive phenotype of oc¹

(Nagao et al., 1998)

Phenotype Manifest In

Suppressor of

Statement

Reference

Hsap\OTX2^hs.PN is a suppressor of embryonic brain & commissure phenotype of oc²

(Adachi et al., 2001)

Hsap\OTX2^hs.PN is a suppressor of embryonic protocerebrum phenotype of oc²

(Adachi et al., 2001)

Hsap\OTX2^hs.PN is a suppressor of ocellus pigment granule phenotype of oc¹

(Nagao et al., 1998)

Hsap\OTX2^hs.PN is a suppressor of ocellus lens phenotype of oc¹

(Nagao et al., 1998)

Hsap\OTX2^hs.PN is a suppressor of adult head bristle phenotype of oc¹

(Nagao et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Partially rescues the embryonic brain defects of oc² animals.

(Adachi et al., 2001)

Rescues the oc¹ phenotype; oc¹ flies carrying Hsap\OTX2^hs.PN have either ocellar lenses and/or associated ocellar pigments. The number of macrochaetae on the vertex of the head is also rescued towards wild-type in these flies.

(Nagao et al., 1998)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

An Hsap\OTX2 cDNA is expressed under the control of a heat shock promoter.

(Leuzinger et al., 1998)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hsap\OTX2^hs.PL

Hsap\OTX2^hs.PN

Name Synonyms

heat shock construct of Nagao

(Nagao et al., 1998)

Secondary FlyBase IDs

FBal0090628

References (3)

Report Sections

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