FB2024_03 , released June 25, 2024
Allele: Dmel\Shab3
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General Information
Symbol
Dmel\Shab3
Species
D. melanogaster
Name
FlyBase ID
FBal0083185
Feature type
allele
Associated gene
Dmel\Shab
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Vahasoyrinki et al., 2006, Hegde et al., 1997)
Mutagen
X ray
(Hegde et al., 1999)
Progenitor genotype
Cytology
Description

Two deletions; 24bp from nucleotides 508 to 531, and 356bp from nucleotides 656 to 1011. The second deletion results in a frame shift, creating a stop codon 74bp downstream of the mutation. The predicted protein is truncated prior to the S1 segment.

(Hegde et al., 1999)

Deletion leading to a frameshift, causing effectively a null mutation.

(Hegde et al., 1997)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3L:2,924,679..2,924,702 [+]
Comment:

The first of two deleted regions in mutant; a 24bp deletion.

(Hegde et al., 1999)
deletion
3L:2,924,827..2,925,182 [+]
Comment:

The second of two deleted regions in mutant; a 356bp deletion which causes a frameshift and early translation termination.

(Hegde et al., 1999)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Loss of Shab in Shab3 mutants removes nearly all of the delayed rectifier K[+] current present in the neurons, while mEPSPs remain stabilised after curare treatment.

      (Ping and Tsunoda, 2011)

      Electrical current recordings from Shab3 mutant photoreceptors show the slowly inactivating current is eliminated, but the transient K+ current is intact in these mutants.

      Shab3 ommatidia exhibit a standard configuration of photoreceptors with normal rhabdoms. In whole-cell recordings, the cell capacitances, which are dominated by the contribution of the microvillar membrane, are indistinguishable from the wild-type, as is the appearance of dissociated ommatidia.

      Shab3 photoreceptors exhibit 'bump' waveforms (elementary responses of the phototransduction machinery corresponding to single-photon absorptions) and impulse responses to light flashes that are similar to wild-type.

      The macroscopic kinetics of Shab3 photoreceptors are not significantly different from those of wild-type photoreceptors.

      Shab3 photoreceptors show reduced steady-state input resistances.

      Shab3 photoreceptors have an input resistance that is significantly reduced from that of wild-type. This decreased input resistance maintains the sensitivity of the photoreceptors to dynamic light intensity fluctuations.

      (Vahasoyrinki et al., 2006)

      Homozygotes are paralysed within 3 minutes at 39oC. They recover from paralysis within 4 minutes of being transferred to 25oC. The delayed rectifier current (IK) is reduced by 61.2 +/- 2.7% compared to wild-type in Shab3 larval muscles.

      (Hegde et al., 1999)

      The voltage-activated delayed sustained current (IK) is partly reduced (by approximately 65%) in Shab3 larval muscles.

      (Singh and Singh, 1999)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Statement
      Reference

      Shab3 has abnormal neurophysiology phenotype, enhanceable by Sh14

      (Vahasoyrinki et al., 2006)
      Suppressed by
      Statement
      Reference

      Shab3 has viable phenotype, suppressible by Sh14

      (Vahasoyrinki et al., 2006)
      Enhancer of
      Statement
      Reference

      Shab3 is an enhancer of abnormal neurophysiology phenotype of Sh14

      (Vahasoyrinki et al., 2006)
      Suppressor of
      Statement
      Reference

      Shab3 is a suppressor of viable phenotype of Sh14

      (Vahasoyrinki et al., 2006)
      Other
      Statement
      Reference

      Sh14, Shab3 has sterile phenotype

      (Vahasoyrinki et al., 2006)
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      Shab3 has photoreceptor phenotype, enhanceable by Sh14

      (Vahasoyrinki et al., 2006)
      Enhancer of
      Statement
      Reference

      Shab3 is an enhancer of photoreceptor phenotype of Sh14

      (Vahasoyrinki et al., 2006)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Sh14; Shab3 double mutants are poorly viable and infertile.

      Examination of Shab3 photoreceptor whole-cell currents reveals that the steady-state current is at least two-times greater than in Sh14; Shab3 double mutants.

      (Vahasoyrinki et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      Shab1

      (Hegde et al., 1999)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      9g
      (Hegde et al., 1999)
      Shab3
      (Ping and Tsunoda, 2011, Vahasoyrinki et al., 2006)
      Shab9g
      (Ueda and Wu, 2006, Hegde et al., 1997)
      Name Synonyms
      Secondary FlyBase IDs
        References (6)