FlyBase Allele Report: Dmel\kuz[k01405]

General Information

Symbol

Dmel\kuz^k01405

Species

D. melanogaster

Name

FlyBase ID

FBal0082859

Feature type

allele

Associated gene

Dmel\kuz

Associated Insertion(s)

P{lacW}kuz^k01405

Carried in Construct

Also Known As

kuz¹⁴⁰⁵

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Sotillos et al., 1997)

Progenitor genotype

Associated Insertion(s)

P{lacW}kuz^k01405

Cytology

Description

Insertion of a P{lacW} element within untranslated leader sequences.

(Sotillos et al., 1997)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:13,550,212..13,550,212 [-]

(Udolph et al., 2009, Koelzer and Klein, 2006, Koelzer and Klein, 2003, Ashburner et al., 1999, Ashburner et al., 1999.10.12, Sotillos et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | somatic clone

(Udolph et al., 2009)

visible | recessive

(Sotillos et al., 1997)

visible | recessive | somatic clone

(Sotillos et al., 1997)

Phenotype Manifest In

Detailed Description

Statement

Reference

kuz^k01405 germ line clones exhibit duplication of the RP2 neurons.

(Udolph et al., 2009)

Homozygotes have severely reduced wings and halteres, small nota, shortened legs with fused tarsal segments and rough eyes. The flies have gross alterations in the pattern of sensory organs, including the appearance of abnormal tufts of bristles or other sensory organs in locations where only one sensory organ or naked cuticle is present in wild-type flies. Cuticle lacking both microchaetae and macrochaetae is also seen. Homozygous clones induced in the dorsal part of the wing at 60-84 hours after egg laying give rise to thickening of the wing veins and nicks at the wing margin. Within the clone a cluster of campaniform sensilla develops at the position of one of the twin sensilla of the margin. kuz^1405Rev4/kuz^k01405 flies show scalloping of the wings. Homozygous larvae have imaginal discs of reduced size. The pupal notum contains lawns of neurons where only one or a few neurons are present in wild-type flies.

(Sotillos et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

kuz^k01405 has visible | recessive phenotype, suppressible by Scer\GAL4^C-765/E(spl)m8-HLH^UAS.cSa

(Sotillos et al., 1997)

kuz^k01405 has visible | recessive phenotype, suppressible by H²

(Sotillos et al., 1997)

kuz^k01405 has visible | recessive phenotype, suppressible by N^int.hs

(Sotillos et al., 1997)

Enhancer of

Statement

Reference

kuz^k01405/kuz^1405Rev4 is an enhancer of visible | dominant phenotype of Ser¹

(Sotillos et al., 1997)

Suppressor of

Statement

Reference

kuz^k01405 is a suppressor of visible phenotype of H²

(Sotillos et al., 1997)

Other

Statement

Reference

Ser¹, kuz^k01405 has visible | recessive phenotype

(Sotillos et al., 1997)

Phenotype Manifest In

Suppressed by

Statement

Reference

kuz^k01405 has dorsal mesothorax phenotype, suppressible by N^int.hs

(Sotillos et al., 1997)

kuz^k01405 has leg phenotype, suppressible by N^int.hs

(Sotillos et al., 1997)

kuz^k01405 has eye phenotype, suppressible by N^int.hs

(Sotillos et al., 1997)

kuz^k01405 has sense organ phenotype, suppressible by Scer\GAL4^C-765/E(spl)m8-HLH^UAS.cSa

(Sotillos et al., 1997)

kuz^k01405 has wing phenotype, suppressible by H²

(Sotillos et al., 1997)

kuz^k01405 has wing phenotype, suppressible by N^int.hs

(Sotillos et al., 1997)

kuz^k01405 has haltere phenotype, suppressible by N^int.hs

(Sotillos et al., 1997)

Enhancer of

Statement

Reference

kuz^k01405/kuz^1405Rev4 is an enhancer of wing phenotype of Ser¹

(Sotillos et al., 1997)

Suppressor of

Statement

Reference

kuz^k01405 is a suppressor of sense organ phenotype of H²

(Sotillos et al., 1997)

NOT Suppressor of

Statement

Reference

kuz^k01405 is a non-suppressor of wing vein phenotype of H²

(Sotillos et al., 1997)

Other

Statement

Reference

Ser¹, kuz^k01405 has wing phenotype

(Sotillos et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

The scalloping of the wing margin seen in Ser^D/+ heterozygotes is enhanced if the flies also carry kuz^1405Rev4/kuz^k01405. The neurogenic phenotype of kuz^k01405 flies is partially rescued by E(spl)^Scer\UAS.cSa expressed under the control of Scer\GAL4^C-765; many single sensory organs, rather than tufts of sensory organs, develop on the notum. The sensory organ but not the wing vein phenotypes of H² heterozygotes and homozygotes are suppressed by kuz^k01405. The neurogenic phenotype and reduced wing size of kuz^k01405 homozygotes is suppressed by H². kuz^k01405 is partially rescued by N^int.hs at 25^oC; kuz^k01405 flies carrying N^int.hs have a normal-sized notum and eyes, and their legs, wings and halteres are substantially larger than those of kuz^k01405 single mutant flies.

(Sotillos et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

kuz^H143

(Sotillos et al., 1997)

Partially rescued by

kuz^k01405 is partially rescued by kuz^UAS.cFa/Scer\GAL4^C-765

(Sotillos et al., 1997)

Comments

The development of wings, halteres, thorax, legs and eyes in homozygotes is rescued by kuz^Scer\UAS.cFa expressed under the control of Scer\GAL4^C-765. The pattern of sensory organs is restored almost to normal in these flies, although several notum microchaetae are missing and extra stout bristles (some with abnormal morphology) are seen on the anterior wing margin.

(Sotillos et al., 1997)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

May be a cluster mate of kuz^k01403.

(Ashburner et al., 1999.10.12)

Comments

Precise excision of the P{lacW} element results in reversion to wild-type.

(Sotillos et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

k01405

(Ashburner et al., 1999)

kuz¹⁴⁰⁵

(Koelzer and Klein, 2006, Koelzer and Klein, 2003, Sotillos et al., 1997)

kuz^k01405

l(2)k01405

(Ashburner et al., 1999.10.12, Sotillos et al., 1997)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

Open Close