FlyBase Allele Report: Dmel\mr[3]

General Information

Symbol

Dmel\mr³

Species

D. melanogaster

Name

FlyBase ID

FBal0065732

Feature type

allele

Associated gene

Dmel\mr

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Reed and Orr-Weaver, 1997)

Progenitor genotype

Cytology

Description

Amino acid replacement: W282term.

(Kashevsky et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:23,973,545..23,973,545 [+]

Nucleotide change:

G23973545A

Amino acid change:

W282term | mr-PA

Reported amino acid change:

W282term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

(Kashevsky et al., 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(van Roessel et al., 2004)

abnormal neurophysiology

(van Roessel et al., 2004)

lethal (with mr⁴)

(Kashevsky et al., 2002)

partially lethal (with mr¹)

(Kashevsky et al., 2002)

Phenotype Manifest In

embryonic/larval neuromuscular junction

(van Roessel et al., 2004)

NMJ bouton | increased number

(van Roessel et al., 2004)

Detailed Description

Statement

Reference

Synapses at the neuromuscular junction are greatly expanded in mr³ homozygous third instar larvae compared to controls. The size of individual boutons is similar at the mutant synapse is similar to that of controls, but the number of boutons per synapse is almost doubled compared to wild type. There is no significant increase in branching at the mutant synapse. The amplitudes of both spontaneous excitatory junctional potentials (mEJPs) and evoked EJPs are greater in homozygous larvae than in controls. Quantal content is not significantly increased in mutant neuromuscular junctions and the frequency of spontaneous events is not significantly altered. No obvious defects in pre- and postsynaptic ultrastructure are seen in mutant neuromuscular junctions and synaptic vesicle size is not significantly altered compared to wild type.

(van Roessel et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

mr¹/mr³ has partially lethal phenotype, enhanceable by CycB^+t10

(Kashevsky et al., 2002)

Suppressed by

Statement

Reference

mr³ has abnormal neuroanatomy phenotype, suppressible by Liprin-α^1J-0/Liprin-α^1J-0

(van Roessel et al., 2004)

mr³ has abnormal neuroanatomy phenotype, suppressible by Liprin-α^1J-0/Liprin-alpha[+]

(van Roessel et al., 2004)

Phenotype Manifest In

Suppressed by

Statement

Reference

mr³ has NMJ bouton | increased number phenotype, suppressible by Liprin-α^1J-0/Liprin-α^1J-0

(van Roessel et al., 2004)

mr³ has NMJ bouton | increased number phenotype, suppressible by Liprin-α^1J-0/Liprin-alpha[+]

(van Roessel et al., 2004)

mr³ has embryonic/larval neuromuscular junction phenotype, suppressible by Liprin-α^1J-0/Liprin-α^1J-0

(van Roessel et al., 2004)

mr³ has embryonic/larval neuromuscular junction phenotype, suppressible by Liprin-α^1J-0/Liprin-alpha[+]

(van Roessel et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of boutons per synapse seen at the neuromuscular junction of mr³ homozygous third instar larvae is strongly suppressed by Liprin-α^1J-0/+ and is completely suppressed by Liprin-α^1J-0/Liprin-α^1J-0.

(van Roessel et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

mr⁴/mr³ is rescued by Scer\GAL4^Act5C.PI/mr^UASp.cKa

(Kashevsky et al., 2002)

Partially rescued by

mr³ is partially rescued by Scer\GAL4^how-24B/mr^UASp.cKa

(van Roessel et al., 2004)

mr³ is partially rescued by mr^UASp.cKa/Scer\GAL4^elav.PLu

(van Roessel et al., 2004)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

mr³

(Slack et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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