FlyBase Allele Report: Dmel\ed[k01102]

General Information

Symbol

Dmel\ed^k01102

Species

D. melanogaster

Name

FlyBase ID

FBal0064604

Feature type

allele

Associated gene

Dmel\ed

Associated Insertion(s)

P{lacW}ed^k01102

Carried in Construct

Also Known As

l(2)k01102, k01102

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}ed^k01102

Cytology

Description

Insertion of P{lacW} into the first intron of ed, upstream of the coding region.

(Bai et al., 2001)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:4,031,769..4,031,769 [+]

(Dworkin and Gibson, 2006, Swan et al., 2006, Swan et al., 2006, Ahmed et al., 2003, Rawlins et al., 2003, Spencer and Cagan, 2003, Bai et al., 2001, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Dworkin and Gibson, 2006, Spradling et al., 1999, BDGP Project Members, 1994-1999)

lethal - all die before end of larval stage | recessive

(Spencer and Cagan, 2003)

lethal - all die before end of pupal stage (with ed^m1)

(Ahmed et al., 2003)

lethal - all die before end of pupal stage | recessive

(Bai et al., 2001)

partially lethal - majority die (with Df(2L)ed-dp)

(Rawlins et al., 2003)

partially lethal - majority die (with ed^6.1)

(Rawlins et al., 2003)

partially lethal - majority die (with ed^lH23)

(Rawlins et al., 2003)

some die during larval stage | recessive

(Spencer and Cagan, 2003)

some die during pupal stage (with Df(2L)ed-dp)

(Rawlins et al., 2003)

some die during pupal stage (with ed^6.1)

(Rawlins et al., 2003)

some die during pupal stage (with ed^lH23)

(Rawlins et al., 2003)

some die during pupal stage (with ed^m1)

(Ahmed et al., 2003)

some die during pupal stage | recessive

(Bai et al., 2001)

visible | dominant

(Dworkin and Gibson, 2006)

Phenotype Manifest In

chemosensory ventral triple row & microchaeta | ectopic (with ed^lH23)

(Rawlins et al., 2003)

dorsal triple row & microchaeta | ectopic (with ed^lH23)

(Rawlins et al., 2003)

dorsocentral bristle | ectopic (with Df(2L)ed-dp)

(Rawlins et al., 2003)

dorsocentral bristle | ectopic (with ed^6.1)

(Rawlins et al., 2003)

dorsocentral bristle | ectopic (with ed^lH23)

(Rawlins et al., 2003)

eye (with ed^m1)

(Ahmed et al., 2003)

photoreceptor cell R7 | somatic clone | increased number

(Spencer and Cagan, 2003)

photoreceptor cell R8 | increased number

(Spencer and Cagan, 2003)

photoreceptor cell R8 | increased number (with Df(2L)ed-dp)

(Rawlins et al., 2003)

photoreceptor cell R8 | increased number (with ed^slA12)

(Spencer and Cagan, 2003)

photoreceptor cell R8 | somatic clone | increased number

(Spencer and Cagan, 2003)

scutellar bristle | ectopic (with Df(2L)ed-dp)

(Rawlins et al., 2003)

scutellar bristle | ectopic (with ed^6.1)

(Rawlins et al., 2003)

scutellar bristle | ectopic (with ed^lH23)

(Rawlins et al., 2003)

sex comb (with ed^m1)

(Ahmed et al., 2003)

wing

(Dworkin and Gibson, 2006)

Detailed Description

Statement

Reference

Heterozygotes show a direct effect on the shape of the wing.

(Dworkin and Gibson, 2006)

ed^k01102/ed^m1 animals usually die as pupae, but adult escapers are sometimes produced in low density cultures. These escapers have mild defects in the leg; the legs have the correct number of segments, but the sex combs are malformed in males. They also have a rough eye phenotype.

(Ahmed et al., 2003)

Twinning of R8 photoreceptor cells is seen in ed^k01102/Df(2L)ed-dp animals.

(Rawlins et al., 2003)

ed^lH23/ed^k01102 flies have extra dorsocentral, scutellar and recurved wing margin bristles (chemosensory ventral triple row + dorsal triple row). ed^6.1/ed^k01102 and ed^k01102/Df(2L)ed-dp flies have extra dorsocentral and scutellar bristles.

(Rawlins et al., 2003)

Homozygotes are viable until the late third larval instar. Homozygous and ed^k01102/ed^slA12 eye discs contain ommatidia with multiple R8 cells. This phenotype is also seen in homozygous clones in mosaic eye discs. Ommatidia in homozygous clones in the eye sometimes contain extra R7 cells and in all cases where ommatidia contain extra R7 cells, extra R8 cells are also present.

(Spencer and Cagan, 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

ed[+]/ed^k01102 is an enhancer of visible | dominant phenotype of Egfr^E1

(Spencer and Cagan, 2003)

Suppressor of

Statement

Reference

ed^k01102 is a suppressor of visible phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

ed[+]/ed^k01102 is a suppressor of visible | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

ed^k01102 has photoreceptor cell R8 | increased number phenotype, enhanceable by aos^W11

(Spencer and Cagan, 2003)

ed^k01102 has photoreceptor cell R8 | increased number phenotype, enhanceable by aos^Δ7/argos[+]

(Spencer and Cagan, 2003)

Enhancer of

Statement

Reference

ed[+]/ed^k01102 is an enhancer of eye phenotype of Egfr^E1

(Spencer and Cagan, 2003)

Suppressor of

Statement

Reference

ed^k01102 is a suppressor of eye phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

ed[+]/ed^k01102 is a suppressor of eye | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

ed^k01102/+ strongly enhances the Egfr^E1/+ phenotype, resulting in eyes that are approximately 1/3 the size of those of Egfr^E1/+ flies. argos^Δ7/+ mildly enhances the ed^k01102 phenotype; the number of ommatidia with multiple R8 cells is increased from 18% to 34% in the double mutant flies. argos^W11 enhances the ed^k01102 phenotype; the number of ommatidia with multiple R8 cells is increased from 21% to 46% in the double mutant flies.