FlyBase Allele Report: Dmel\nrv2[k13315]

General Information

Symbol

Dmel\nrv2^k13315

Species

D. melanogaster

Name

FlyBase ID

FBal0064138

Feature type

allele

Associated gene

Dmel\nrv2

Associated Insertion(s)

P{lacW}nrv2^k13315

Carried in Construct

Also Known As

l(2)k13315

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}nrv2^k13315

Cytology

Description

Inserted in to the second intron of nrv2.

(Genova and Fehon, 2003)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:6,792,138..6,792,138 [+]

(Esmangart de Bournonville et al., 2024, Paul et al., 2003, Kaufmann et al., 2002, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Shirasaki et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal (with Df(2L)ED6569)

(Kahsai et al., 2016)

lethal | recessive

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Phenotype Manifest In

Detailed Description

Statement

Reference

The assembly, integrity and stability of septate junctions are impaired and the midbody fails to move basally in nrv2^k13315 clones of dividing epithelial cells.

nrv2^k13315 clones of epithelial cells lack finger-like protrusions normally associated with the midbody of either wild-type or nrv2^k13315 neighboring dividing cells.

(Daniel et al., 2018)

Homozygous clones in the third larval instar tracheal system are only occasionally recovered after they have been induced at the late blastoderm/early gastrulation stage.

(Baer et al., 2007)

In nrv2^k13315 homozygous mutants the adherens junction remains intact, with the regular spacing of plasma membranes maintained. However, the septae normally located between these membranes are reduced in number or absent. The paracellular diffusion barrier is disrupted in salivary glands of nrv2^k13315 homozygous embryos. nrv2^k13315/nrv2^k13315 somatic clones generated in larvae (approx. 72 hours after egg laying) do not survive in adult tissues.

(Genova and Fehon, 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

nrv2^k13315 is an enhancer of abnormal locomotor behavior | adult stage | progressive phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^elav.PU

(Shirasaki et al., 2012)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

nrv2^k13315 has embryonic salivary gland & epithelial cell phenotype, non-suppressible by nrv1^UAS.Tag:FLAG/Scer\GAL4^e22c

(Genova and Fehon, 2003)

nrv2^k13315/nrv2^k04223 has presumptive embryonic/larval tracheal system phenotype, non-suppressible by nrv1^UAS.cPa/Scer\GAL4^e22c

(Paul et al., 2003)

nrv2^k13315/nrv2^23B has presumptive embryonic/larval tracheal system phenotype, non-suppressible by Scer\GAL4^e22c/nrv3^UAS.cPa

(Paul et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

When expressed under the control of Scer\GAL4^e22c, nrv1^{Scer\UAS.T:Zzzz\FLAG} does not rescue the dye diffusion phenotype across the salivary gland epithelium observed in nrv2^k13315 mutants.

(Genova and Fehon, 2003)

nrv2^k13315/nrv2^k04223 tracheal phenotypes are not suppressed by nrv1^Scer\UAS.cPa; Scer\GAL4^e22c. nrv2^k13315/nrv2^23B tracheal phenotypes are not suppressed by nrv3^Scer\UAS.cPa; Scer\GAL4^e22c.

(Paul et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

nrv2^k04223

(Paul et al., 2003)

Rescued by

nrv2^k13315 is rescued by nrv2^{2.UAS.Tag:FLAG}/Scer\GAL4^e22c

(Genova and Fehon, 2003)

nrv2^k13315 is rescued by Scer\GAL4^e22c/nrv2^{1.UAS.Tag:FLAG}

(Genova and Fehon, 2003)

nrv2^k13315/nrv2^k04223 is rescued by nrv2^2.UAS/Scer\GAL4^e22c

(Paul et al., 2003)

Partially rescued by

nrv2^k13315/nrv2^k04223 is partially rescued by nrv2^1.UAS/Scer\GAL4^btl.PS

(Paul et al., 2003)

nrv2^k13315/nrv2^k04223 is partially rescued by nrv2^1.UAS/Scer\GAL4^e22c

(Paul et al., 2003)

nrv2^k13315/nrv2^k04223 is partially rescued by nrv2^2.UAS/Scer\GAL4^btl.PS

(Paul et al., 2003)

Comments

When expressed under the control of Scer\GAL4^e22c, nrv2^{1.Scer\UAS.T:Zzzz\FLAG} rescues the dye diffusion phenotype across the salivary gland epithelium observed in nrv2^k13315 mutants.

(Genova and Fehon, 2003)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

11086

y¹ w^67c23; P{lacW}nrv2^k13315/CyO

Kyoto

114351

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{lacW}nrv2^k13315 P{neoFRT}40A/CyO y⁺

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Comments

Complements: Rca1⁰³³⁰⁰. Complements: nrv2^k04223.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

l(2)k13315

(Genova and Fehon, 2003, Spradling et al., 1999)

l(2)k13315^k13315

(Kaufmann et al., 2002, BDGP Project Members, 1994-1999)

nrv2^k13315

(Esmangart de Bournonville et al., 2024, Daniel et al., 2018, Tempesta et al., 2017, Shirasaki et al., 2012, Stork et al., 2009, Stork et al., 2008, Baer et al., 2007)

nrv2^l(2)k13315

(Paul et al., 2003)

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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