FlyBase Allele Report: Dmel\eg[UAS.cDa]

General Information

Symbol

Dmel\eg^UAS.cDa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Dittrich

FlyBase ID

FBal0062864

Feature type

allele

Associated gene

Dmel\eg

Associated Insertion(s)

Carried in Construct

P{UAS-eg.D}

Also Known As

UAS-eg

Key Links

Transgenic product class

wild_type

(Dittrich et al., 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Dittrich et al., 1997)

Mutagen

in vitro construct

(Dittrich et al., 1997)

Progenitor genotype

Carried in construct

P{UAS-eg.D}

Cytology

Description

The entire eg coding region is expressed under the control of UASt regulatory sequences.

(Dittrich et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^kni.L2

(Lunde et al., 2003)

Phenotype Manifest In

embryo | dorsal closure stage, with Scer\GAL4^pnr-MD237

(Gritzan et al., 2002)

embryonic dorsal epidermis, with Scer\GAL4^pnr-MD237

(Gritzan et al., 2002)

serotonergic neuron | ectopic, with Scer\GAL4^sca-537.4

(Dittrich et al., 1997)

wing sensillum | ectopic, with Scer\GAL4^kni.L2

(Lunde et al., 2003)

Detailed Description

Statement

Reference

Expression of eg^Scer\UAS.cDa under the control of Scer\GAL4^kni.L2 results in ectopic sensory bristles along the L2 wing vein.

(Lunde et al., 2003)

Expression of eg^Scer\UAS.cDa under the control of Scer\GAL4^pnr-MD237 results in a dorsal open phenotype.

(Gritzan et al., 2002)

The neuropile is disorganised in embryos expressing eg^Scer\UAS.cDa under the control of Scer\GAL4^sca-537.4. Most embryos expressing eg^Scer\UAS.cDa under the control of Scer\GAL4^sca-537.4 hatch, giving rise to first instar larvae with 1 to 2 ectopic serotonergic cells in most hemisegments. First instar larvae derived from embryos expressing eg^Scer\UAS.cDa under the control of Scer\GAL4^elav-C155 or Scer\GAL4^en-e16E do not contain ectopic serotonergic cells.

(Dittrich et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

eg^UAS.cDa/Scer\GAL4^kni.L2 is a suppressor of visible phenotype of kni^ri-1

(Lunde et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^pnr-MD237, eg^UAS.cDa has embryonic dorsal epidermis phenotype, enhanceable by Scer\GAL4^pnr-MD237

(Gritzan et al., 2002)

Scer\GAL4^pnr-MD237, eg^UAS.cDa has embryo | dorsal closure stage phenotype, enhanceable by Scer\GAL4^pnr-MD237

(Gritzan et al., 2002)

Suppressed by

Statement

Reference

Scer\GAL4^pnr-MD237, eg^UAS.cDa has embryo | dorsal closure stage phenotype, suppressible by Scer\GAL4^pnr-MD237

(Gritzan et al., 2002)

Scer\GAL4^sca-537.4, eg^UAS.cDa has serotonergic neuron | ectopic phenotype, suppressible by hkb²

(Dittrich et al., 1997)

Suppressor of

Statement

Reference

eg^UAS.cDa/Scer\GAL4^eg-Mz360 is a suppressor of serotonin neuron & ventral nerve cord phenotype of robo2^X123

(Couch et al., 2004)

eg^UAS.cDa/Scer\GAL4^kni.L2 is a suppressor of wing vein L2 phenotype of kni^ri-1

(Lunde et al., 2003)

Other

Statement

Reference

Scer\GAL4^sca-537.4, eg^UAS.cDa, hkb² has serotonergic neuron phenotype

(Dittrich et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of eg^Scer\UAS.cDa under the control of Scer\GAL4^kni.L2 restores wing vein L2 formation in kni^ri-1 flies. The restored L2 vein is decorated with a line of ectopic sensory bristles (these ectopic bristles are also seen when eg^Scer\UAS.cDa is expressed under the control of Scer\GAL4^kni.L2 in a wild-type background).

(Lunde et al., 2003)

The dorsal open phenotype caused by expression of eg^Scer\UAS.cDa under the control of Scer\GAL4^pnr-MD237 is enhanced by kay¹. The dorsal closure phenotype caused by expression of eg^Scer\UAS.cDa under the control of Scer\GAL4^pnr-MD237 is suppressed by puc^E69.

(Gritzan et al., 2002)

Most of the ectopic serotonergic cells and most of the wild-type serotonergic cells are missing if larvae expressing eg^Scer\UAS.cDa under the control of Scer\GAL4^sca-537.4 are also homozygous for hkb².

(Dittrich et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

eg^Scer\UAS.cDa

eg^UAS.cDa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Dittrich

(Dittrich et al., 1997)

Secondary FlyBase IDs

References (5)

Report Sections

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