FlyBase Allele Report: Dmel\rux[hs.PT]

General Information

Symbol

Dmel\rux^hs.PT

Species

D. melanogaster

Name

heat shock construct of Thomas

FlyBase ID

FBal0060902

Feature type

allele

Associated gene

Dmel\rux

Associated Insertion(s)

Carried in Construct

P{Hs-rux}

Also Known As

HS-rux

Key Links

Transgenic product class

cDNA

(Thomas et al., 1997)

wild_type

(Thomas et al., 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Thomas et al., 1997)

wild_type

(Thomas et al., 1997)

Mutagen

in vitro construct

(Sprenger et al., 1997, Thomas et al., 1997)

Progenitor genotype

Carried in construct

P{Hs-rux}

Cytology

Description

An Hsp70 promoter drives expression of a full-length rux cDNA.

(Thomas et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

rux

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle

(Vidwans et al., 2002)

abnormal size | embryonic stage

(Vidwans et al., 2002)

Phenotype Manifest In

mitotic cell cycle

(Vidwans et al., 2002)

nucleus | embryonic stage

(Vidwans et al., 2002)

Detailed Description

Statement

Reference

The expression of rux^hs.PT during G2₁₄ leads to a delay in M-phase 14, and then S phase 15. Consistent with these nuclei having passed through M-phase 14 they are the size of interphase 15 nuclei and exhibit normal ploidy.

(Vidwans et al., 2002)

Expression is wild type flies blocks mitosis.

(Thomas et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

rux^hs.PT is a suppressor of abnormal mitotic cell cycle phenotype of enc^unspecified

(Ohlmeyer and Schupbach, 2003)

Other

Statement

Reference

CycE^AR95, rux^hs.PT has abnormal mitotic cell cycle phenotype

(Vidwans et al., 2002)

Phenotype Manifest In

Suppressor of

Statement

Reference

rux^hs.PT is a suppressor of egg chamber phenotype of enc^unspecified

(Ohlmeyer and Schupbach, 2003)

rux^hs.PT is a suppressor of mitotic cell cycle phenotype of CycA^UAS.Δ170, Scer\GAL4^prd.RG1

(Foley and Sprenger, 2001)

Cdk1^AF.hs/rux^hs.PT is a suppressor of mitotic cell cycle phenotype of CycA^hs.PK

(Foley et al., 1999)

rux^hs.PT is a suppressor of mitotic cell cycle phenotype of Cdk1^AF.hs, CycA^hs.PK

(Foley et al., 1999)

Other

Statement

Reference

CycE^AR95, rux^hs.PT has mitotic cell cycle phenotype

(Vidwans et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The extra mitotic division phenotype seen in the egg chambers of enc^unspecified females at 25^oC is partially suppressed by rux^hs.PT; only 25% of egg chambers contain 32 cells. This suppression of enc^unspecified by rux^hs.PT is not significantly altered if the females also carry CycA^unspecified/+.

(Ohlmeyer and Schupbach, 2003)

The addition of CycA^{Scer\UAS.Δ170}, induced by heatshock, is sufficient to overcome the metaphase block seen in CycA^{Scer\UAS.Δ170},Scer\GAL4^prd.RG1 embryos.

(Foley and Sprenger, 2001)

Suppresses the induction of mitosis seen in CycA^hs.PK, cdc2^AF.hs embryos.

(Foley et al., 1999)

CycA^hs.PK flies exhibit ectopic S phase entry in the morphogenetic furrow, rux^hs.PT inhibits the ectopic S phase entry at the morphogenetic furrow but not in anterior or posterior cells. CycE^hs.PR flies exhibit ectopic S phases in the eye disc, coexpression of rux^hs.PT fails to suppress this phenotype.

(Thomas et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

rux^hs.PT

Name Synonyms

heat shock construct of Thomas

(Thomas et al., 1997)

Secondary FlyBase IDs

References (6)

Report Sections

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