FB2024_03 , released June 25, 2024
Allele: Dmel\Hsc70-4K71S.UAS
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General Information
Symbol
Dmel\Hsc70-4K71S.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0060645
Feature type
allele
Associated gene
Dmel\Hsc70-4
Associated Insertion(s)
Carried in Construct
P{UAS-Hsc70-4.K71S}
Also Known As
UAS-Hsc4.K71S, UAS-Hsc70-4K71S, UAS-HSC70-4.K71S
Key Links
Transgenic product class
missense_variant
(Elefant and Palter, 1999)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Elefant and Palter, 1999)
Mutagen
in vitro construct
(Elefant and Palter, 1999, Elefant and Palter, 1997)
Progenitor genotype
Hsc70-4UAS.cEa
(Elefant and Palter, 1999)
Carried in construct
P{UAS-Hsc70-4.K71S}
Cytology
Description

Amino acid replacement: K71S.

(Elefant and Palter, 1999)

Encodes a Hsc70-4 protein with a single point mutation in the ATP binding site.

(Elefant and Palter, 1997)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Hsc70-4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Hsc70-4K71S.Scer\UAS under the control of Scer\GAL4btl.PS results in disorganisation of the chitin extracellular matrix in the embryonic tracheal lumens. Lumen clearance is defective in these embryos, resulting in a failure of gas filling.

      (Behr et al., 2007)

      Expression of Hsc70-4K71S.Scer\UAS under the control of Scer\GAL4GMR.PF results in a rough eye phenotype.

      (Hagedorn et al., 2006)

      Expression of Hsc70-4K71S.Scer\UAS under the control of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C results in increased cell size in imaginal discs.

      (Hennig et al., 2006)

      Expression of Hsc70-4K71S.Scer\UAS, under the regulation of Scer\GAL4GMR.PF, during eye development results in disruption of the regular arrays of ommatidia and a roughening of the eye.

      (Chang et al., 2004)

      A modest effect on the eye is seen when Hsc70-4K71S.Scer\UAS is driven by Scer\GAL4GMR.PF.

      (Jin et al., 2003)

      Expression of Hsc70-4K71S.Scer\UAS under the control of Scer\GAL4GMR.PF does not result in a rough eye phenotype.

      (Shulman and Feany, 2003)

      20 day old (but not 1 day old) flies expressing Hsc70-4K71S.Scer\UAS under the control of Scer\GAL4Ddc.PL show loss of dopaminergic neurons.

      (Auluck et al., 2002)

      Animals expressing Hsc70-4K71S.Scer\UAS under the control of Scer\GAL4337Y die as first or second instar larvae. Animals expressing Hsc70-4K71S.Scer\UAS under the control of Scer\GAL4how-24B generally die as pupae or larvae (depending on the P{UAS-Hsc70-4.K71S} line used).

      (Elefant and Palter, 1999)

      Expression of Hsc70-4K71S.Scer\UAS under the control of Scer\GAL4GMR.PF has no effect on the eye.

      (Warrick et al., 1999)

      Dominant lethal allele.

      (Elefant and Palter, 1997)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT suppressed by
      Enhancer of
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Statement
      Reference

      Hsc70-4K71S.UAS, Scer\GAL4GMR.PF is a non-suppressor of visible phenotype of Hsap\MAPTV337M.UAS, Scer\GAL4GMR.PF

      (Shulman and Feany, 2003)
      Phenotype Manifest In
      Enhanced by
      NOT Enhanced by
      NOT suppressed by
      Enhancer of
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The mechanical stress sensitivity of Tpisgk-1 animals is significantly suppressed if they are also expressing Hsc70-4K71S.Scer\UAS under the control of Scer\GAL4Act5C.Switch.PR (in the presence of Mifepristone).

      (Hrizo and Palladino, 2010)

      Expression of Hsc70-4K71S.Scer\UAS under the control of Scer\GAL4GMR.PF in a auxI670K/+ background generates a severe rough eye phenotype, more severe than expression of Hsc70-4K71S.Scer\UAS under the control of Scer\GAL4GMR.PF in a wild-type background.

      (Hagedorn et al., 2006)

      Co-expression of Hsc70-4K71S.Scer\UAS and Rme-8fl.Scer\UAS.T:Disc\RFP-mRFP, under the regulation of Scer\GAL4GMR.PF, during eye development enhances the ommatidial disorganisation observed in Hsc70-4K71S.Scer\UAS (regulated by Scer\GAL4GMR.PF). In addition, a decrease in eye size is also observed, suggesting that the rough eye phenotype of Hsc70-4K71S.Scer\UAS (regulated by Scer\GAL4GMR.PF) is enhanced by Rme-8fl.Scer\UAS.T:Disc\RFP-mRFP expression. Expression of Rme-8ΔJ.Scer\UAS.T:Disc\RFP-mRFP, under the regulation of Scer\GAL4GMR.PF has little effect on the Hsc70-4K71S.Scer\UAS (under the control of Scer\GAL4GMR.PF) phenotype. Expression of auxillinfl.Scer\UAS, under the regulation of Scer\GAL4GMR.PF, has little or no effect on the rough eye phenotype of Hsc70-4K71S.Scer\UAS (expressed under the control of Scer\GAL4GMR.PF).

      (Chang et al., 2004)
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression of Hsc70-4K71S.Scer\UAS with Scer\GAL4GMR.PF>Hsap\GJB3F137L.Scer\UAS.T:Hsap\MYC leads to further deleterious eye phenotypes manifesting in complete loss of pigmentation and increased black degeneration lesions.

      (Tang et al., 2015)

      Co-expression of BacA\p35Scer\UAS.cHa suppresses the Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 induced loss of rhabdomeres and significantly reduces the percentage of cells with aggregates.

      (Wong et al., 2008)

      The progressive degeneration and pigmentation loss eye phenotype characteristic for flies expressing Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PU is enhanced by co-expression of Hsc70-4K71S.Scer\UAS.

      (Huen and Chan, 2005)

      Expression of Hsc70-4K71S.Scer\UAS in flies expressing Hsap\HDQ108.ex1p.Scer\UAS, both under the control of Scer\GAL4elav-C155, increases the survival rate from almost 0% to over 80%.

      The presence of the Hsc70-4K71S.Scer\UAS transgene does partially suppress Hsap\HDQ108.ex1p.Scer\UAS-mediated locomotor dysfunction (when both are expressed under the control of Scer\GAL4elav-C155).

      Expression of Hsc70-4K71S.Scer\UAS in flies expressing Hsap\HDQ108.ex1p.Scer\UAS under the control of Scer\GAL4elav-C155 in a CrebB-17A+tIa transgene background increases the survival rate from approximately 10% in Hsap\HDQ108.ex1p.Scer\UAS (Scer\GAL4elav-C155) CrebB-17A+tIa mutants, to over 100%, indicating a synergistic effect between Hsc70-4K71S.Scer\UAS and CrebB-17A+tIa.

      (Iijima-Ando et al., 2005)

      The rough eye phenotype caused by expression of Hsap\MAPTV337M.Scer\UAS under the control of Scer\GAL4GMR.PF is not modified if the flies are also carrying Hsc70-4K71S.Scer\UAS.

      (Shulman and Feany, 2003)

      Co-expression of Hsc70-4K71S.Scer\UAS accelerates the loss of dopaminergic neurons caused by expression of Hsap\SNCAScer\UAS.cFa under the control of Scer\GAL4Ddc.PL; neuronal loss is apparent in 1 day old flies expressing both Hsc70-4K71S.Scer\UAS and Hsap\SNCAScer\UAS.cFa under the control of Scer\GAL4Ddc.PL whereas neuronal loss in flies overexpressing only Hsap\SNCAScer\UAS.cFa under the control of Scer\GAL4Ddc.PL occurs by 10 to 20 days.

      (Auluck et al., 2002)

      Co-expression of Hsc70-4K71S.Scer\UAS with Hsap\ARQ112.Scer\UAS.T:Ivir\HA1 in the eye (under the control of Scer\GAL4GMR.PF enhances the eye phenotype, such that the flies show a severely degenerated eye, whereas expression of Hsap\ARQ112.Scer\UAS.T:Ivir\HA1 alone mildly affects the eye.

      Co-expression of Pros261.Scer\UAS and Hsc70-4K71S.Scer\UAS, together with the pathogenic Hsap\ARQ112.Scer\UAS.T:Ivir\HA1 lines (all under the control of the Scer\GAL4GMR.PF driver) enhances the eye phenotype.

      (Chan et al., 2002)

      Expression of DnaJ-1Scer\UAS.T:Zzzz\FLAG cannot suppress the severe eye degeneration seen in flies co-expressing Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 and Hsc70-4K71S.Scer\UAS under the control of Scer\GAL4GMR.PU.

      (Chan et al., 2000)

      Enhances the disruption of the eye caused by expression of Hsap\MJDQ78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF.

      (Warrick et al., 1999)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (3)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (6)
      Reported As
      Symbol Synonym
      HSC4K71S
      (Elefant and Palter, 1997)
      Hsc4DN
      (Ghosh and Feany, 2004)
      Hsc70-4K71S.Scer\UAS
      Hsc70-4K71S.UAS
      Hsc70-4K71S
      (Chang et al., 2004)
      K71S
      (Elefant and Palter, 1995)
      Name Synonyms
      Secondary FlyBase IDs
        References (22)