FlyBase Allele Report: Dmel\Tm2[D53]

General Information

Symbol

Dmel\Tm2^D53

Species

D. melanogaster

Name

FlyBase ID

FBal0060550

Feature type

allele

Associated gene

Dmel\Tm2

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: S185F. Nucleotide substitution: C?T.

(Naimi et al., 2001)

Amino acid replacement: S185F.

(Sparrow et al., 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:15,313,006..15,313,006 [+]

Nucleotide change:

C15313006T

Reported nucleotide change:

C?T

Amino acid change:

Reported amino acid change:

S185F

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Naimi et al., 2001, Sparrow et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight | dominant

(Naimi et al., 2001)

Phenotype Manifest In

Detailed Description

Statement

Reference

Tm2^D53 mutants do not show abnormal behaviour at 38^oC.

(Montana and Littleton, 2004)

Heterozygotes and homozygotes have a small, significant reduction in flight ability which is age independent (wing beat frequency is reduced). The walking speed of Tm2^D53 flies is very similar to wild type and does not change with age.

(Naimi et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Tm2^D53 is a suppressor of lethal phenotype of Mhc^S1

(Montana and Littleton, 2004)

Tm2^D53/Tm2[+] is a suppressor of abnormal jumping phenotype of wupA^hdp-2

(Naimi et al., 2001)

Tm2^D53/Tm2^D53 is a suppressor of abnormal jumping phenotype of wupA^hdp-2

(Naimi et al., 2001)

Tm2^D53 is a suppressor of abnormal locomotor behavior | larval stage phenotype of wupA^hdp-2

(Naimi et al., 2001)

Tm2^D53/Tm2[+] is a suppressor of abnormal locomotor behavior phenotype of wupA^hdp-2

(Naimi et al., 2001)

Tm2^D53/Tm2[+] is a suppressor of abnormal locomotor behavior phenotype of up¹⁰¹

(Naimi et al., 2001)

Other

Statement

Reference

Tm2^D53/Tm2[+], up¹⁰¹ has flightless phenotype

(Naimi et al., 2001)

Tm2^D53/Tm2[+], wupA^hdp-2 has abnormal flight phenotype

(Naimi et al., 2001)

Tm2^D53, wupA^hdp-2 has abnormal flight phenotype

(Naimi et al., 2001)

Tm2^D53, up¹⁰¹ has flightless phenotype

(Naimi et al., 2001)

Phenotype Manifest In

Suppressor of

Statement

Reference

Tm2^D53 is a suppressor of indirect flight muscle cell phenotype of Mhc^S1

(Montana and Littleton, 2004)

Tm2^D53/Tm2^D53 is a suppressor of indirect flight muscle cell phenotype of wupA^hdp-2

(Naimi et al., 2001)

Tm2^D53/Tm2[+] is a suppressor of indirect flight muscle cell phenotype of wupA^hdp-2

(Naimi et al., 2001)

Tm2^D53/Tm2[+] is a suppressor of somatic muscle cell of leg phenotype of wupA^hdp-2

(Naimi et al., 2001)

Tm2^D53/Tm2^D53 is a suppressor of indirect flight muscle cell phenotype of up¹⁰¹

(Naimi et al., 2001)

Tm2^D53/Tm2[+] is a suppressor of indirect flight muscle cell phenotype of up¹⁰¹

(Naimi et al., 2001)

Tm2^D53/Tm2^D53 is a suppressor of wing phenotype of up¹⁰¹

(Naimi et al., 2001)

Tm2^D53/Tm2[+] is a suppressor of wing phenotype of up¹⁰¹

(Naimi et al., 2001)

Tm2^D53 is a suppressor of phenotype of wupA^hdp-2

(Sparrow et al., 1997)

NOT Suppressor of

Statement

Reference

Tm2^D53 is a non-suppressor of indirect flight muscle cell phenotype of Mhc¹³

(Naimi et al., 2001)

Tm2^D53 is a non-suppressor of indirect flight muscle cell phenotype of wupA^hdp-3

(Naimi et al., 2001)

Tm2^D53 is a non-suppressor of indirect flight muscle cell phenotype of wupA^hdp-5

(Naimi et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Mhc^S1/+ structural defects are partially suppressed in Tm2^D53 mutants, as the indirect flight muscles of double mutants display less degradation despite the presence of indented thoraces compared to similarly aged Mhc^S1/+ flies. Tm2^D53 suppresses the recessive lethality of Mhc^S1, increasing homozygote viability from 2.38% to 80.92%.

(Montana and Littleton, 2004)

The hypercontraction of indirect flight muscles seen in wupA^hdp-2 flies is completely suppressed by Tm2^D53/+. IFMs of 2-3 day old wupA^hdp-2 ; Tm2^D53/+ flies show an almost complete restoration of wild-type myofibrillar structure. However, some myofibrils show small areas of disorganised filament lattice at their centres. By 6-7 days, all of the myofibrils contain extensive disorganised areas. wupA^hdp-2 myofibrillar defects are completely suppressed by Tm2^D53/Tm2^D53. wupA^hdp-2 flies that also carry one or two copies of Tm2^D53 hold their wings in the resting position. Young wupA^hdp-2 ; Tm2^D53/+ or wupA^hdp-2 ; Tm2^D53/Tm2^D53 flies can fly, but less well than wild type. By 6-7 days old, the flight ability of wupA^hdp-2 ; Tm2^D53/+ but not wupA^hdp-2 ; Tm2^D53/Tm2^D53 flies is dramatically reduced. wupA^hdp-2 ; Tm2^D53/+ flies show some suppression of the wupA^hdp-2 leg muscle phenotype. The larval locomotory and feeding behaviour defects of wupA^hdp-2 larvae are suppressed by Tm2^D53. The up¹⁰¹ wings-up and IFM phenotypes are completely suppressed by Tm2^D53/Tm2^D53 and partially suppressed by Tm2^D53/+. In both cases, the rescued flies are flightless.

(Naimi et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

D53

(Naimi et al., 2001)

Tm2^D53

Name Synonyms

Secondary FlyBase IDs

References (4)

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