FB2024_03 , released June 25, 2024
Allele: Dmel\cup01355
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General Information
Symbol
Dmel\cup01355
Species
D. melanogaster
Name
FlyBase ID
FBal0060458
Feature type
allele
Associated gene
Dmel\cup
Associated Insertion(s)
P{PZ}cup01355
Carried in Construct
Also Known As
cup1355, fs(2)01355
Key Links
Allele class

hypomorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - molecular evidence

(Keyes and Spradling, 1997)
Mutagen
P-element activity
(Keyes and Spradling, 1997)
Progenitor genotype
Associated Insertion(s)
P{PZ}cup01355
Cytology
Description

Insertion of a P{PZ} element within the untranslated region of the first exon.

(Keyes and Spradling, 1997)
Allele components
Component
Use(s)
Inserted element
P{PZ}
Encoded product / tool
lacZ
Tagged with
Tag:NLS(P)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Egg chamber maturation proceeds normally in homozygous females.

      (Grimaldi et al., 2007)

      Egg chambers arrest at stage S14. Nurse cell nuclear chromatin in the oldest egg chambers appears normal. The mature egg chambers have an open chorion.

      (Keyes and Spradling, 1997)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Other
      Statement
      Reference

      Nup154tlp2, cup01355 has ovariole phenotype

      (Grimaldi et al., 2007)

      Nup154tlp2, cup01355 has chromosome & nurse cell phenotype

      (Grimaldi et al., 2007)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      GALTΔ1V2/+ genetically complements +/cup01355.

      GALTΔ1AP2/+ genetically complements +/cup01355.

      GALTC2/+ genetically complements +/cup01355.

      (Kushner et al., 2010)

      The ovarioles of cup01355 Nup154tlp2 double homozygous females show a "string of pearls" morphology due to the arrest of egg-chamber development at around stage 4-5. Stage 6-7 egg chambers are only occasionally produced in the double mutant females. In these egg chambers, virtually all nurse cell nuclei have abnormal chromatin configurations that range from tight condensed "spiral" chromosomes (never seen in wild-type nurse cells) to "blob-like" chromosomes (transiently assumed by chromosome arms in wild type prior to their normal dispersal).

      (Grimaldi et al., 2007)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      cup01355/cup3 is rescued by Scer\GAL4VP16.nanos.UTR/cupUAS.cNa

      (Nelson et al., 2004)
      Partially rescued by

      cup01355/cup3 is partially rescued by Scer\GAL4VP16.nanos.UTR/cupMT.UAS.cNa

      (Nelson et al., 2004)
      Comments

      cup01355/cupKG00049 females crossed to wild-type males produce viable embryos that develop into phenotypically normal flies.

      (Kushner et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Mobilisation of the P{PZ} insertion in cup01355 produces many fertile revertants, indicating that the P{PZ} insertion is responsible for the cup01355 phenotype.

      (Keyes and Spradling, 1997)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      References (11)