FlyBase Allele Report: Dmel\U2af38[ΔE18]

General Information

Symbol

Dmel\U2af38^ΔE18

Species

D. melanogaster

Name

FlyBase ID

FBal0058042

Feature type

allele

Associated gene

Dmel\U2af38

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

loss of function allele

(Rudner et al., 1998, Rudner et al., 1996)

Mutagen

Delta2-3 transposase

(Rudner et al., 1996)

Progenitor genotype

U2af38⁰⁶⁷⁵¹

(Rudner et al., 1996)

Cytology

Description

Imprecise excision of the P-element,resulting in a 950bp deletion that removes approximately two-thirds of the U2af38 coding sequence.

(Rudner et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Rudner et al., 1998)

lethal - all die before end of larval stage | recessive

(Rudner et al., 1996)

some die during larval stage | recessive

(Rudner et al., 1996)

Phenotype Manifest In

embryonic/larval crystal cell

(Milchanowski et al., 2004)

Detailed Description

Statement

Reference

The average number of crystal cells per embryo is reduced in homozygous stage 13-14 embryos compared to wild type.

(Milchanowski et al., 2004)

Homozygotes die during the first larval instar period.

(Rudner et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Sxl^fP7B0, U2af38^ΔE18, snf^J210 has lethal phenotype

(Nagengast et al., 2003)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The female progeny of U2af38^ΔE18/+ females mated to Sxl^fP7B0/Y males are completely viable.

The female progeny of U2af38^ΔE18/fl(2)d¹ females mated to Sxl^fP7B0/Y males show only 25.8% viability.

The female progeny of U2af38^ΔE18/fl(2)d² females mated to Sxl^fP7B0/Y males show only 90.1% viability.

(Penn et al., 2008)

When mothers heterozygous for both snf^J210 and U2af38^ΔE18 are crossed with Sxl^fP7B0 males, a significant reduction in viability is seen in Sxl^fP7B0/+ daughters.

(Nagengast et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

U2af38^ΔE18 is rescued by U2af38^ΔGly

(Rudner et al., 1998)

U2af38^ΔE18 is rescued by U2af38^+t10

(Rudner et al., 1998)

U2af38^ΔE18 is rescued by U2af38^ΔRSGly

(Rudner et al., 1998)

U2af38^ΔE18 is rescued by U2af38^ΔRS

(Rudner et al., 1998)

U2af38^ΔE18 is rescued by U2af38^+t10

(Rudner et al., 1996)

Not rescued by

U2af38^ΔE18 is not rescued by U2af38^hs.PR

(Rudner et al., 1996)

U2af38^ΔE18 is not rescued by U2af38^U2af50.PR

(Rudner et al., 1996)

U2af38^ΔE18 is not rescued by U2af38^tRb

(Rudner et al., 1996)

Comments

U2af38^ΔE18 U2af50^XR15 double mutants can be rescued by a combination of U2af50^+tS12 and U2af38^+t10 but not by a combination of U2af50^ΔRS and U2af38^ΔRS. Lethal combination could both be rescued by an identical synthetic RS domain by a combination of U2af50^RS.7 and U2af38^ΔRS.

(Rudner et al., 1998)

Homozygous lethality is rescued by U2af38^+t10, but not by U2af38^tRb, U2af38^hs.PR or U2af38^U2af50.PR. The lethality is partially rescued by U2af38^tRb in combination with U2af38^U2af50.PR.

(Rudner et al., 1996)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

U2af38^DE18

(Milchanowski et al., 2004)

U2af38^ΔE18

(Johnson et al., 2010, Penn et al., 2008)

ΔE18

(Rudner et al., 1996)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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