FlyBase Allele Report: Dmel\dap[UAS.cLa]

General Information

Symbol

Dmel\dap^UAS.cLa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Lane

FlyBase ID

FBal0057654

Feature type

allele

Associated gene

Dmel\dap

Associated Insertion(s)

Carried in Construct

P{UAS-dap.L}

Also Known As

UAS-Dap, UAS-Dacapo

Key Links

Transgenic product class

cDNA

(Lane et al., 1996)

wild_type

(Lane et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Lane et al., 1996)

wild_type

(Lane et al., 1996)

Mutagen

in vitro construct

(Calvi et al., 1998, Lane et al., 1996)

Progenitor genotype

Carried in construct

P{UAS-dap.L}

Cytology

Description

UASt regulatory sequences drive expression of dap cDNA sequences.

(Lane et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dap

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates brain cancer

modeled by aurA⁸⁸³⁹

(Wang et al., 2006)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell cycle, with Scer\GAL4^en.PU

(Wong et al., 2014)

abnormal cell cycle, with Scer\GAL4^hh.PU

(Wong et al., 2014)

abnormal mitotic cell cycle, with Scer\GAL4^neur-P72

(Remaud et al., 2008, Audibert et al., 2005)

abnormal neuroanatomy, with Scer\GAL4^sca-537.4

(Berger et al., 2005)

abnormal size, with Scer\GAL4^ey.PH

(Gupta et al., 2008)

decreased occurrence of cell division | larval stage, with Scer\GAL4^GMR.PU

(Zielke et al., 2006)

decreased occurrence of cell division | larval stage, with Scer\GAL4^ptc-559.1

(Legent et al., 2006)

female sterile, with Scer\GAL4^c323

(Calvi et al., 1998)

visible, with Scer\GAL4^GMR.PF

(Gupta et al., 2008)

visible, with Scer\GAL4^ptc-559.1

(Legent et al., 2006)

visible, with Scer\GAL4^vg.int2.1

(Legent et al., 2006)

Phenotype Manifest In

adult abdomen | somatic clone, with Scer\GAL4^Act5C.PI

(Ninov et al., 2007)

cardioblast, with Scer\GAL4^twi.PG

(Han and Bodmer, 2003)

dorsal appendage, with Scer\GAL4^c323

(Calvi et al., 1998)

egg, with Scer\GAL4^c323

(Calvi et al., 1998)

egg chorion, with Scer\GAL4^c323

(Calvi et al., 1998)

embryonic/larval epidermis | somatic clone, with Scer\GAL4^Act5C.PI

(Ninov et al., 2007)

embryonic pericardial cell, with Scer\GAL4^eve.eme

(Han and Bodmer, 2003)

embryonic pericardial cell, with Scer\GAL4^twi.PG

(Han and Bodmer, 2003)

eye, with Scer\GAL4^GMR.PF

(Gupta et al., 2008)

eye disc posterior to the morphogenetic furrow | somatic clone, with Scer\GAL4^GMR.PU

(Zielke et al., 2006)

G1 phase & external sensory organ precursor cell IIa, with Scer\GAL4^neur-P72

(Audibert et al., 2005)

G1 phase & external sensory organ precursor cell IIb, with Scer\GAL4^neur-P72

(Audibert et al., 2005)

histoblast | somatic clone, with Scer\GAL4^Act5C.PI

(Ninov et al., 2007)

neuron & embryonic thorax, with Scer\GAL4^sca-537.4

(Berger et al., 2005)

thoracic neuroblast NB6-4, with Scer\GAL4^sca-537.4

(Berger et al., 2005)

wing, with Scer\GAL4^vg.int2.1

(Legent et al., 2006)

wing disc, with Scer\GAL4^ptc-559.1

(Legent et al., 2006)

wing margin, with Scer\GAL4^ptc-559.1

(Legent et al., 2006)

Detailed Description

Statement

Reference

Overexpression of dap^Scer\UAS.cLa in the imaginal discs, under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU, leads to G[[1]] cell cycle arrest.

(Wong et al., 2014)

Expression of dap^Scer\UAS.cLa under the control of Scer\GAL4^GMR.PF results in a small eye phenotype.

(Gupta et al., 2008)

S-phase in secondary precursor cells in the bristle lineage is delayed by around 20 minutes by expression of dap^Scer\UAS.cLa under the control of Scer\GAL4^neur-P72.

(Remaud et al., 2008)

Histoblast clones that express dap^Scer\UAS.cLa under the control of Scer\GAL4^Act5C.PI do not proliferate. This results in a profound delay in larval epidermis cell death. Developmental timing is not affected in these animals but pharate adult esacpers show multiple differentiation and morphogenetic abdominal defects.

(Ninov et al., 2007)

The distance between wing veins L3 and L4 at the wing margin is reduced in animals expressing dap^Scer\UAS.cLa under the control of Scer\GAL4^ptc-559.1.

There is a decrease in S phase cells in cells expressing dap^Scer\UAS.cLa under the control of Scer\GAL4^ptc-559.1 in the wing disc.

Expression of dap^Scer\UAS.cLa under the control of Scer\GAL4^vg.int2.1 results in nicks in the wing.

(Legent et al., 2006)

Over-expression of dap^Scer\UAS.cLa under the control of Scer\GAL4^GMR.PU blocks the second wave of mitosis posterior to the morphogenetic furrow in the eye imaginal disc.

(Zielke et al., 2006)

Overexpression of dap^Scer\UAS.cLa under the control of Scer\GAL4^neur-P72 triggers a G1-phase in pIIa and pIIb cells.

(Audibert et al., 2005)

Overexpression of dap^Scer\UAS.cLa, under the regulation of Scer\GAL4^sca-537.4 results in a reduction in the number of neurons in the NB6-4t lineage (from the wild-type number of 6 to 2-4 in approximately 81% of hemisegments), but homeotic transformation of the lineage does not occur. In addition, the number of glial cells is not affected, neither in the thorax nor in the abdomen.

(Berger et al., 2005)

When dap^Scer\UAS.cLa is driven by Scer\GAL4^twi.PG, most hemisegments of mutant embryos show only one eve expressing pericardial cell (EPC), suggesting that the last division generating two EPCs did not occur. A reduction is also seen in the number of myocardial cells in these mutants. When dap^Scer\UAS.cLa is driven by Scer\GAL4^eve.eme, both DA1 and DO2 founders form, but the number of EPCs is reduced to half.

(Han and Bodmer, 2003)

Females expressing dap^Scer\UAS.cLa under the control of Scer\GAL4^c323 are sterile, and lay flaccid eggs with thin eggshells and smaller dorsal appendages than normal.

(Calvi et al., 1998)

Scer\GAL4^F4-mediated expression results in inhibition of endoreplication.

(Weiss et al., 1998)

Scer\GAL4^prd.RG1 mediated overexpression results in a stable G1 arrest.

(Lane et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^vg.int2.1, dap^UAS.cLa has visible phenotype, enhanceable by vg[+]/vg^null

(Legent et al., 2006)

Suppressed by

Statement

Reference

dap^UAS.cLa, vg[+]/vg^null has decreased occurrence of cell division | larval stage phenotype, suppressible by vg^UAS.cZa/Scer\GAL4^vg.int2.1

(Delanoue et al., 2004)

Scer\GAL4^vg.int2.1, dap^UAS.cLa, vg[+]/vg^null has visible phenotype, suppressible by vg^UAS.cZa/Scer\GAL4^vg.int2.1

(Delanoue et al., 2004)

Enhancer of

Statement

Reference

dap^UAS.cLa, Scer\GAL4^ey.PH is an enhancer of visible phenotype of Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

dap^UAS.cLa, Scer\GAL4^ey.PH is an enhancer of abnormal size phenotype of Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Suppressor of

Statement

Reference

dap^UAS.cLa, Scer\GAL4^hh.PU is a suppressor of increased cell death phenotype of BubR1^VDRC.cUa, Scer\GAL4^hh.PU, bsk^KK108156

(Wong et al., 2014)

dap^UAS.cLa, Scer\GAL4^en.PU is a suppressor of increased cell death phenotype of BubR1^VDRC.cUa, Scer\GAL4^en.PU, bsk^KK108156

(Wong et al., 2014)

dap^UAS.cLa/Scer\GAL4^wor.PA is a suppressor of abnormal neuroanatomy phenotype of aurA⁸⁸³⁹

(Wang et al., 2006)

dap^UAS.cLa/Scer\GAL4^Act5C.PP is a suppressor of increased occurrence of cell division | somatic clone | larval stage phenotype of Scer\GAL4^Act5C.PP, ci^UAS.cDa

(Duman-Scheel et al., 2002)

NOT Suppressor of

Statement

Reference

dap^UAS.cLa/Scer\GAL4^vg.PM is a non-suppressor of lethal | pupal stage phenotype of Scer\GAL4^vg.PM, stwl^UY823

(Brun et al., 2006)

dap^UAS.cLa, Scer\GAL4^GMR.PU is a non-suppressor of increased occurrence of cell division | larval stage phenotype of Rca1^{UAS.Tag:HA.Tag:FLAG}, Scer\GAL4^GMR.PU

(Zielke et al., 2006)

Other

Statement

Reference

Scer\GAL4^vg.int2.1, dap^UAS.cLa, vg[+]/vg^null has visible phenotype

(Delanoue et al., 2004)

dap^UAS.cLa, vg[+]/vg^null has decreased occurrence of cell division | larval stage phenotype

(Delanoue et al., 2004)

Scer\GAL4^vg.PU, dap^UAS.cLa, vg[+]/vg^null has visible phenotype

(van de Bor et al., 1999)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^vg.int2.1, dap^UAS.cLa has wing phenotype, enhanceable by vg[+]/vg^null

(Legent et al., 2006)

Suppressed by

Statement

Reference

Scer\GAL4^vg.int2.1, dap^UAS.cLa, vg[+]/vg^null has wing phenotype, suppressible by vg^UAS.cZa/Scer\GAL4^vg.int2.1

(Delanoue et al., 2004)

Scer\GAL4^prd.RG1, dap^UAS.cLa has cell cycle phenotype, suppressible by CycE^UAS.cLa, Scer\GAL4^prd.RG1

(Lane et al., 1996)

Enhancer of

Statement

Reference

dap^UAS.cLa, Scer\GAL4^ey.PH is an enhancer of eye phenotype of Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Suppressor of

Statement

Reference

dap^UAS.cLa, Scer\GAL4^hh.PU is a suppressor of wing disc phenotype of BubR1^VDRC.cUa, Scer\GAL4^hh.PU, bsk^KK108156

(Wong et al., 2014)

dap^UAS.cLa, Scer\GAL4^en.PU is a suppressor of wing disc phenotype of BubR1^VDRC.cUa, Scer\GAL4^en.PU, bsk^KK108156

(Wong et al., 2014)

dap^UAS.cLa/Scer\GAL4^wor.PA is a suppressor of neuroblast | supernumerary & larval brain phenotype of aurA⁸⁸³⁹

(Wang et al., 2006)

dap^UAS.cLa/Scer\GAL4^Act5C.PP is a suppressor of S phase | ectopic | somatic clone phenotype of Scer\GAL4^Act5C.PP, ci^UAS.cDa

(Duman-Scheel et al., 2002)

dap^UAS.cLa/Scer\GAL4^Act5C.PP is a suppressor of eye disc morphogenetic furrow | somatic clone phenotype of Scer\GAL4^Act5C.PP, ci^UAS.cDa

(Duman-Scheel et al., 2002)

NOT Suppressor of

Statement

Reference

dap^UAS.cLa, Scer\GAL4^GMR.PU is a non-suppressor of eye disc posterior to the morphogenetic furrow | somatic clone phenotype of Rca1^{UAS.Tag:HA.Tag:FLAG}, Scer\GAL4^GMR.PU

(Zielke et al., 2006)

Other

Statement

Reference

Scer\GAL4^vg.int2.1, dap^UAS.cLa, vg[+]/vg^null has wing phenotype

(Delanoue et al., 2004)

Scer\GAL4^vg.PU, dap^UAS.cLa, vg[+]/vg^null has wing phenotype

(van de Bor et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Overexpression of dap^Scer\UAS.cLa in the imaginal discs does not affect the amount of apoptosis seen in BubR1^VDRC.cUa knockdown cells (with all transgenes under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU).

Overexpression of dap^Scer\UAS.cLa in the imaginal discs greatly reduces the amount of apoptosis seen in bsk^KK108156 and BubR1^VDRC.cUa knockdown cells (with all transgenes under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU).

(Wong et al., 2014)

The severity of the wing nicking phenotype caused by expression of dap^Scer\UAS.cLa under the control of Scer\GAL4^vg.int2.1 is greatly enhanced by vg^null/+.

(Legent et al., 2006)

Over-expression of Rca1^{Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG} and dap^Scer\UAS.cLa under the control of Scer\GAL4^GMR.PU does not alter the ability of Rca1^{Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG} to induce ectopic cell division in the eye disc.

(Zielke et al., 2006)

Expression of dap^Scer\UAS.cLa along the dorsal/ventral boundary of the wing under the control of Scer\GAL4^vg.int2.1 in a vg^null/+ mutant background results in nicks at the wing tips. There is a significant decrease in mitotic cells along the dorsal/ventral boundary.

Co-expression of vg^Scer\UAS.cZa in flies expressing dap^Scer\UAS.cLa at the dorsal/ventral wing boundary under the control of Scer\GAL4^vg.int2.1 in a vg^null/+ mutant background rescues wing morphology, and mitotic activity of cells is restored in this region.

(Delanoue et al., 2004)

Co-expression of dap^Scer\UAS.cLa blocks the ability of ci^Scer\UAS.cDa (expressed under the control of Scer\GAL4^Act5C.PP) to induce S phase in clones in the morphogenetic furrow.

(Duman-Scheel et al., 2002)

Expression of dap^Scer\UAS.cLa under the control of Scer\GAL4^vg.PU in a vg^null/+ background results in a mutant wing phenotype.

(van de Bor et al., 1999)

Simultaneous expression of CycE^Scer\UAS.cLa completely rescues the cell cycle arrest induced by Scer\GAL4^prd.RG1 mediated overexpression of dap^Scer\UAS.cLa.

(Lane et al., 1996)

Xenogenetic Interactions

Statement

Reference

Expression of dap^Scer\UAS.cLa greatly enhances the eye phenotypes seen when Mmus\Shisa5^Scer\UAS.cGa is expressed under the control of Scer\GAL4^ey.PH.

(Gupta et al., 2008)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Incorporation of BrdU is undetectable in virtually all follicle cells in egg chambers of females expressing dap^Scer\UAS.cLa under the control of Scer\GAL4^c323 or Scer\GAL4^hs.PB.

(Calvi et al., 1998)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

dap^Scer\UAS.cLa

dap^UAS.cLa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Lane

(Lane et al., 1996)

Secondary FlyBase IDs

References (27)

Report Sections

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