8bp deletion of nucleotides 665 to 672 that introduces a frameshift.
P{HS-PN+4} fails to rescue the lethal interaction with awdK, P{HS-PN+3} does rescue the lethality (with or without heat shock) and male progeny are recovered.
pnhs.4 fails to rescue the lethal interaction of pn12C with awdK. pnhs.3 does rescue the lethality (with or without heat shock) and male progeny are recovered. Eye colour of P{HS-PN+4} transformants remains pn even after several heat pulses, P{HS-PN+3} transformants (without heat shock or with one or numerous heat shocks) exhibit wild-type colouration.