FB2024_03 , released June 25, 2024
Allele: Dmel\vnγ4
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General Information
Symbol
Dmel\vnγ4
Species
D. melanogaster
Name
FlyBase ID
FBal0050936
Feature type
allele
Associated gene
Dmel\vn
Associated Insertion(s)
Carried in Construct
Key Links
Allele class

loss of function allele

amorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Schnepp et al., 1996, Simcox et al., 1996)

loss of function allele

(Szuts et al., 1998)
Mutagen
gamma ray
(Simcox et al., 1996)
Progenitor genotype
Caused by aberration
T(2;3)vnγ4
Cytology
Description

Disrupts the vn transcribed region, breaking in the major intron, intron 1.

(Schnepp et al., 1996)

Translocation breakpoint is in the first intron.

(Simcox et al., 1996)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      vnγ4/Df(3L)γ3 stage 13/14 embryos show a reduction in longitudinal glia proliferation compared to wild type (assayed using pHis3 expression).

      (Griffiths and Hidalgo, 2004)

      vnγ4/Df(3L)γ3 embryos have various defects in dMP2 fascicles. At stage 14 this fascicle may be absent altogether, it may fuse with the pCC fascicle, or it may fasciculate with the aCC axon and exit the central nervous system (CNS). Older mutant embryos show defasciculation within the CNS particularly of the second and third longitudinal fascicles. vnγ4/Df(3L)γ3 embryos have reduced numbers of longitudinal (interface) glial cells. The frequency and extent of apoptosis of this cell type is significantly greater than that seen in wild-type measured as either: 1. the percentage of embryos with more than one apoptotic longitudinal glial cell (around 80% for wild-type and 50% for mutant (0.025>p>0.05 by ξ2)); or 2. the overall incidence of apoptosis (p>0.001, Mann-Whitney U test.).

      (Hidalgo et al., 2001)

      Null allele. Shows strongest midgut phenotype of vn alleles tested. Causes lack of midgut constrictions and failure of elongation of gastric caeca.

      (Szuts et al., 1998)

      In vnγ4/Df(3L)γ3 mutants 50% of the Keilin's organs are missing. Head region is normal. Most die as embryos or larvae, but 5% survive to pupariate, with pupal cases shorter and slimmer than wild type.

      (Schnepp et al., 1996)

      Individuals transheterozygous with a vn deletion or with a strong EMS vn mutation exhibit small wings. Transheterozygotes with vnL6 are pupal/pharate adult lethal.

      (Simcox et al., 1996)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      vnγ4 has embryonic longitudinal glial cell phenotype, enhanceable by spi1

      (Hidalgo et al., 2001)
      Suppressed by
      Statement
      Reference

      vnγ4 has embryonic longitudinal glial cell phenotype, suppressible by Scer\GAL4htl.POS/BacA\p35UAS.cHa

      (Hidalgo et al., 2001)

      Df(3L)γ3/vnγ4 has wing disc phenotype, suppressible by EgfrE1

      (Schnepp et al., 1996)
      NOT suppressed by
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The decrease in interface glial cell numbers seen in vnγ4/Df(3L)γ3 is partially suppressed by BacA\p35Scer\UAS.cHa; Scer\GAL4htl.POS, but is unaffected by BacA\p35Scer\UAS.cHa; Scer\GAL4elav.PLu. The decrease in interface glial cell numbers and increase in interface glial cell apoptosis seen in vnγ4/Df(3L)γ3 are both enhanced by spi1/spi1.

      (Hidalgo et al., 2001)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescued by

      Df(3L)γ3/vnγ4 is partially rescued by vnUAS.cSa/Scer\GAL4elav.PLu

      (Hidalgo et al., 2001)
      Comments

      Interface glial cell survival in vnγ4/Df(3L)γ3 embryos is rescued by vnScer\UAS.cSa; Scer\GAL4elav.PLu.

      (Hidalgo et al., 2001)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      dddγ4
      (Simcox, 1994)
      veinγ4
      (Griffiths and Hidalgo, 2004)
      vnγ4
      (Moses et al., 2011)
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0028142
      References (8)