FlyBase Allele Report: Dmel\tkv[UAS.cNa]

General Information

Symbol

Dmel\tkv^UAS.cNa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Nellen

FlyBase ID

FBal0050627

Feature type

allele

Associated gene

Dmel\tkv

Associated Insertion(s)

Carried in Construct

P{UAS-tkv.Na}

Also Known As

UAS-tkv

Key Links

Transgenic product class

cDNA

(Nellen et al., 1996)

wild_type

(Nellen et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Nellen et al., 1996)

wild_type

(Nellen et al., 1996)

Mutagen

in vitro construct

(Nellen et al., 1996)

Progenitor genotype

Carried in construct

P{UAS-tkv.Na}

Cytology

Description

UASt regulatory sequences drive expression of a wild-type tkv cDNA.

(Nellen et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

tkv

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape | third instar larval stage, with Scer\GAL4^ap-md544

(Widmann and Dahmann, 2009)

visible, with Scer\GAL4^Act5C.PI

(Funakoshi et al., 2001)

visible, with Scer\GAL4^dpp.blk1

(Tanimoto et al., 2000)

Phenotype Manifest In

wing, with Scer\GAL4^dpp.blk1

(Tanimoto et al., 2000)

wing, with Scer\GAL4^ptc-559.1

(Shen et al., 2008)

wing, with Scer\GAL4^Tub.PU

(Crickmore and Mann, 2006)

wing, with Scer\GAL4^Tub.PU, Scer\GAL4^vg.int2.1

(Crickmore and Mann, 2006)

wing | prepupal stage, with Scer\GAL4^ptc-559.1

(Shen et al., 2008)

wing disc, with Scer\GAL4^Tub.PU

(Crickmore and Mann, 2006)

wing disc | third instar larval stage, with Scer\GAL4^ap-md544

(Widmann and Dahmann, 2009)

wing pouch, with Scer\GAL4^ptc-559.1

(Shen et al., 2008)

wing sensillum, with Scer\GAL4^Act5C.PI

(Funakoshi et al., 2001)

wing vein, with Scer\GAL4^Act5C.PI

(Funakoshi et al., 2001)

wing vein L4, with Scer\GAL4^dpp.blk1

(Tanimoto et al., 2000)

wing vein L5, with Scer\GAL4^dpp.blk1

(Tanimoto et al., 2000)

Detailed Description

Statement

Reference

In early third instar larvae, expression of tkv^Scer\UAS.cNa in the dorsal wing disc under the control of Scer\GAL4^ap-md544 results in an apical-basal cell length 1.4 times longer than in control cells. Expressing cells are pseudostratified, and the apical cell circumference is decreased compared to control cells. The shape of the prospective dorsal hinge cells are not altered. In late third instar larval wing discs, dorsal pouch cells are moderately longer along their apical-basal axis compared with control cells.

(Widmann and Dahmann, 2009)

Anterior overexpression of tkv^Scer\UAS.cNa by Scer\GAL4^ptc-559.1 results in ectopic apical fold formation along the anteroposterior boundary of the larval wing pouch leading to gap formation in the prepupal and adult wing.

(Shen et al., 2008)

The wings of flies that express tkv^Scer\UAS.cNa under the control of Scer\GAL4^tub are ~30% smaller than wild-type wings.

(Crickmore and Mann, 2006)

Expression of tkv^Scer\UAS.cNa in the wing and haltere, driven by Scer\GAL4^tub and Scer\GAL4^vg.int2.1, results in wings that are smaller than wild-type flies. Cell size in tkv^Scer\UAS.cNa-overexpressing wings is the same as for wild-type wings. Furthermore, wing disc size is reduced in larvae expressing tkv^Scer\UAS.cNa under the control of only Scer\GAL4^tub.

(Crickmore and Mann, 2006)

Expression of tkv^Scer\UAS.cNa under the control of Scer\GAL4^Act5C.PI in clones in the wing results in bifurcation of veins and transformation of bristles.

(Funakoshi et al., 2001)

Expression of tkv^Scer\UAS.cNa under the control of Scer\GAL4^dpp.blk1 results in small wings with abnormal patterning in the central region of the wing. Veins 4 and 5 appear to partially fuse and there is abnormal overgrowth of the region between veins 3 and 4.

(Tanimoto et al., 2000)

Coexpression of P{UAS-tkv} and P{UAS-put.N} with Scer\GAL4^69B causes strong dorsalisation of the embryo. Such embryos fail to develop ventral denticle belts and exhibit dorsal hairs along the entire dorsoventral axis. Expression of P{UAS-tkv} alone with Scer\GAL4^69B has no effect on embryonic patterning.

(Nellen et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^Tub.PU, tkv^UAS.cNa has wing phenotype, enhanceable by dpp^hr4/dpp^d6

(Crickmore and Mann, 2006)

Enhancer of

Statement

Reference

tkv^UAS.cNa/Scer\GAL4^Tub.PU is an enhancer of wing phenotype of dpp^hr4/dpp^d6

(Crickmore and Mann, 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of tkv^Scer\UAS.cNa under the control of Scer\GAL4^Act5C in a Ubx^pbx-1/Ubx^9.22 background reduces the size of the P compartment of haltere discs relative to that observed in Ubx^pbx-1/Ubx^9.22 discs (from 1.45 to 0.83), although this ratio is still greater than that of wild-type discs.

(Crickmore and Mann, 2006)

Flies that express tkv^Scer\UAS.cNa, under the control of Scer\GAL4^tub, in a dpp^hr4/dpp^d6 background, have smaller wings than flies expressing the transgene in a wild-type background and than dpp^hr4/dpp^d6 flies that do not express tkv^Scer\UAS.cNa.

(Crickmore and Mann, 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

tkv^UAS.cNa/Scer\GAL4^Tub.PU rescues tkv^xtr

(Gibson and Perrimon, 2005)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

tkv^Scer\UAS.cNa

tkv^UAS.cNa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Nellen

(Nellen et al., 1996)

Secondary FlyBase IDs

References (17)

Report Sections

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