FlyBase Allele Report: Dmel\per[hs.PEa]

General Information

Symbol

Dmel\per^hs.PEa

Species

D. melanogaster

Name

heat shock construct a of Ewer

FlyBase ID

FBal0050250

Feature type

allele

Associated gene

Dmel\per

Associated Insertion(s)

Carried in Construct

P{hsp70-per7.2}

Key Links

Transgenic product class

wild_type

(Ewer et al., 1988)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Ewer et al., 1988)

Mutagen

in vitro construct

(Frisch et al., 1994, Ewer et al., 1990, Ewer et al., 1988)

Progenitor genotype

Carried in construct

P{hsp70-per7.2}

Cytology

Description

7.2kb per construct under the control of an Hsp70 promoter.

(Frisch et al., 1994)

Expression of a 4.9kb EcoRI-HindIII per fragment fused to a 2.3kb HindIII-SalI per fragment (entire per coding sequence, 340bp of the first intron and 2.1kb DNA 3' to the site of transcription termination) is driven by a 450bp XbaI-EcoRI fragment of Hsp70 promoter (sequences necessary for heat inducible transcription).

(Ewer et al., 1990)

Expression of a 6.8kb XbaI-EcoRI fragment of per (300bp of the first intron and the complete coding region) is driven by a 450bp XbaI-EcoRI fragment of an Hsp70 promoter.

(Ewer et al., 1988)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

per

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mating rhythm

(Sakai and Ishida, 2001)

abnormal memory | heat sensitive

(Sakai et al., 2004)

Phenotype Manifest In

Detailed Description

Statement

Reference

In wild-type males, 5 hours of courtship conditioning is insufficient to reduce courtship behaviour 5 days later. However, it is sufficient to cause such reduction in per^hs.PEa males heat shocked before conditioning. In these heat shocked males there is no reduction in courtship behaviour during conditioning, suggesting that the effect is due to improved long term memory of courtship conditioning, rather than an effect on courtship behaviour itself.

(Sakai et al., 2004)

After 7 days of light dark (LD) entrainment, when the mating activity is measured in these flies on the second day of dark dark (DD) cycling thee is no difference seen in mating activities between circadian time (CT) 12 and CT18. However, after heatshock the mating activity at CT12 is significantly lower than at CT18.

(Sakai and Ishida, 2001)

When reared at 18^oC adults exhibit arrhythmic behaviour. When shifted to 29^oC adult behaviour is rhythmic.

(Frisch et al., 1994)

Shifting from 18 to 19^oC at different stages of the life cycle demonstrates rhythmic adult behaviour can only be obtained if per expression is induced in the adult. The action of per after light:dark entrainment seems to be sufficient for activity rhythms to be exhibited in constant darkness.

(Ewer et al., 1988)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

per^hs.PEa has abnormal memory | heat sensitive phenotype, non-enhanceable by CrebB^b.hs

(Sakai et al., 2004)

NOT suppressed by

Statement

Reference

per^hs.PEa has abnormal memory | heat sensitive phenotype, non-suppressible by CrebB^b.hs

(Sakai et al., 2004)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

CrebB-17A^b.hs does not affect the reduction in courtship activity seen in per^hs.PEa males five days after 5 hours of courtship conditioning preceded by heat shock.

(Sakai et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

per^hs.PEa rescues per⁰¹

(Sakai et al., 2004)

Comments

per^hs.PEa rescues the loss of long term memory in per⁰¹ males as assayed by reduction in mating activity in the presence of 5 days after conditioning for 7 hours as long as heat shock is given just before conditioning.

(Sakai et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

per^hs.PEa

Name Synonyms

heat shock construct a of Ewer

(Ewer et al., 1988)

Secondary FlyBase IDs

References (6)

Report Sections

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