7.2kb per construct under the control of an Hsp70 promoter.
Expression of a 4.9kb EcoRI-HindIII per fragment fused to a 2.3kb HindIII-SalI per fragment (entire per coding sequence, 340bp of the first intron and 2.1kb DNA 3' to the site of transcription termination) is driven by a 450bp XbaI-EcoRI fragment of Hsp70 promoter (sequences necessary for heat inducible transcription).
Expression of a 6.8kb XbaI-EcoRI fragment of per (300bp of the first intron and the complete coding region) is driven by a 450bp XbaI-EcoRI fragment of an Hsp70 promoter.