FlyBase Allele Report: Dmel\oc[uvi]

General Information

Symbol

Dmel\oc^uvi

Species

D. melanogaster

Name

UV-insensitive

FlyBase ID

FBal0050218

Feature type

allele

Associated gene

Dmel\oc

Associated Insertion(s)

Carried in Construct

Also Known As

otd^uvi

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

X ray

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(van den Dries et al., 1996)

Mutagen

X ray

(van den Dries et al., 1996)

Progenitor genotype

Cytology

Description

1.5kb deletion in intron 3.

(van den Dries et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal visual behavior | recessive

(van den Dries et al., 1996)

Phenotype Manifest In

Detailed Description

Statement

Reference

Remodelling of the photoreceptor zona adherens occurs 60h after puparium formation (APF)in oc^uvi mutants compared to 44 hours APF in wild type. Photoreceptors exhibit defective elongation along the lens to brain axis. Rhabdomere morphogenesis defects are also seen; on average 1.8 cells per ommatidium harbour a split rhabdomere and 1.2 cells lack a rhabdomere altogether. An increased number of stalk membranes are shorter than 2500nm compared to wild type.

(Fichelson et al., 2012)

Rhabdomeres are misshapen (they are often split and/or duplicated) and are restricted to the distal-most third of the retina in mutant flies. The organisation of the ommatidia is disrupted.

(Terrell et al., 2012)

Rhabdomeres fail to extend more than one-third of the depth of the retina in mutant flies and are often misshapen or duplicated. Ommatidial organisation is disrupted.

(McDonald et al., 2010)

The process of rhabdomere initiation has begun in mutant photoreceptor cells at 60 hours after puparium formation (APF), as in wild-type photoreceptors. However, the mutant photoreceptors show clear disorganisation of the initial stages of rhabdomere elaboration. At 72 hours APF, continuing defects in rhabdomere morphogenesis are seen. At 96 hours APF discernible rhabdomere structures are present, but they are smaller than normal and misshapen.

The rhabdomeres of the ocelli in mutant flies show little difference from those in wild-type controls.

(Mishra et al., 2010)

The shape of the rhabdomeres of all eight photoreceptor cells is abnormal in oc^uvi mutants.

(Tahayato et al., 2003)

Flies are preferentially attracted to visible light over UV light. Rhabdomeres are disorganised and often appear duplicated. The rhabdomere phenotype becomes progressively worse in more proximal sections, resulting in premature rhabdomere termination. The R8 photoreceptor is frequently abnormally localized. Heterozygotes with oc¹ show neither an R-cell nor an ocellar phenotype.

(van den Dries et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

oc^uvi has rhabdomere of eye photoreceptor cell phenotype, enhanceable by Pph13^hazy

(Mishra et al., 2010)

Suppressed by

Statement

Reference

Scer\GAL4^oc.1.6, oc^uvi has photoreceptor phenotype, suppressible | partially by Kr-h1^GD4367, Scer\GAL4^oc.1.6

(Fichelson et al., 2012)

Scer\GAL4^oc.1.6, oc^uvi has rhabdomere phenotype, suppressible | partially by Kr-h1^GD4367, Scer\GAL4^oc.1.6

(Fichelson et al., 2012)

Scer\GAL4^oc.1.6, oc^uvi has photoreceptor phenotype, suppressible | partially by Kr-h1^KK112003, Scer\GAL4^oc.1.6

(Fichelson et al., 2012)

Scer\GAL4^oc.1.6, oc^uvi has rhabdomere phenotype, suppressible | partially by Kr-h1^KK112003, Scer\GAL4^oc.1.6

(Fichelson et al., 2012)

oc^uvi has rhabdomere phenotype, suppressible by Hsap\CRX^UAS.Tag:FLAG/Scer\GAL4^oc.1.6

(Terrell et al., 2012)

oc^uvi has ommatidium phenotype, suppressible by Hsap\CRX^UAS.Tag:FLAG/Scer\GAL4^oc.1.6

(Terrell et al., 2012)

oc^uvi has rhabdomere phenotype, suppressible by Hsap\CRX^{R90W.UAS.Tag:FLAG}/Scer\GAL4^oc.1.6

(Terrell et al., 2012)

oc^uvi has ommatidium phenotype, suppressible by Hsap\CRX^{R90W.UAS.Tag:FLAG}/Scer\GAL4^oc.1.6

(Terrell et al., 2012)

oc^uvi has rhabdomere phenotype, suppressible by Hsap\CRX^{I138fs48.UAS.Tag:FLAG}/Scer\GAL4^oc.1.6

(Terrell et al., 2012)

oc^uvi has ommatidium phenotype, suppressible by Hsap\CRX^{I138fs48.UAS.Tag:FLAG}/Scer\GAL4^oc.1.6

(Terrell et al., 2012)

oc^uvi has rhabdomere phenotype, suppressible by Scer\GAL4^oc.1.6/Hsap\CRX^{E80A.UAS.Tag:FLAG}

(Terrell et al., 2012)

oc^uvi has ommatidium phenotype, suppressible by Scer\GAL4^oc.1.6/Hsap\CRX^{E80A.UAS.Tag:FLAG}

(Terrell et al., 2012)

oc^uvi has rhabdomere phenotype, suppressible by Hsap\OTX1^UAS.Tag:FLAG/Scer\GAL4^oc.1.6

(Terrell et al., 2012)

oc^uvi has ommatidium phenotype, suppressible by Hsap\OTX1^UAS.Tag:FLAG/Scer\GAL4^oc.1.6

(Terrell et al., 2012)

oc^uvi has rhabdomere phenotype, suppressible by Scer\GAL4^oc.1.6/Hsap\OTX2^UAS.Tag:FLAG

(Terrell et al., 2012)

oc^uvi has ommatidium phenotype, suppressible by Scer\GAL4^oc.1.6/Hsap\OTX2^UAS.Tag:FLAG

(Terrell et al., 2012)

NOT suppressed by

Statement

Reference

oc^uvi has rhabdomere phenotype, non-suppressible by Scer\GAL4^oc.1.6/Hsap\CRX^{K88N.UAS.Tag:FLAG}

(Terrell et al., 2012)

oc^uvi has ommatidium phenotype, non-suppressible by Scer\GAL4^oc.1.6/Hsap\CRX^{K88N.UAS.Tag:FLAG}

(Terrell et al., 2012)

Enhancer of

Statement

Reference

oc^uvi is an enhancer of rhabdomere of eye photoreceptor cell phenotype of Pph13^hazy

(Mishra et al., 2010)

Other

Statement

Reference

Pph13^hazy, oc^uvi has eye photoreceptor cell phenotype

(Mishra et al., 2010)

Pph13^hazy, oc^uvi has ocellus rhabdomere phenotype

(Mishra et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Kr-h1^GD4367 under the control of Scer\GAL4^oc.1.6 partially suppresses the apical membrane maturation defects seen in oc^uvi mutant photoreceptors. The photoreceptor elongation defects, rhabdomere loss and stalk membrane length are all also partially suppressed.

Expression of Kr-h1^KK112003 under the control of Scer\GAL4^oc.1.6 partially suppresses the apical membrane maturation defects seen in oc^uvi mutant photoreceptors.

(Fichelson et al., 2012)

The process of rhabdomere initiation has begun in photoreceptor cells at 60 hours after puparium formation (APF) in oc^uvi ; Pph13^hazy double mutants, as in wild-type photoreceptors. However, the mutant photoreceptors show clear disorganisation of the initial stages of rhabdomere elaboration. At 72 hours APF the defects in rhabdomere morphogenesis in the double mutant are more severe than in either single mutant; the apical rhabdomeric membrane lacks any definable shape and organisation in the double mutant. By 96 hours APF no rhabdomere is recognisable in the double mutants. Instead, there is a region of apical membrane that extends out into the inter-rhabdomeral space in distal portions of the retina, but this membrane lacks any organised structure. This membrane structure is visible along the entire length of the photoreceptor cell. No photoreceptor cell structures can be discerned beyond approximately 20μm from the surface of the retina, compared with 100μm for a wild-type photoreceptor cell.

oc^uvi ; Pph13^hazy double mutants lack rhabdomeres in the ocelli photoreceptor cells.

(Mishra et al., 2010)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\OTX1^{Scer\UAS.T:Zzzz\FLAG} or Hsap\OTX2^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^oc.1.6 rescues rhabdomere elongation in oc^uvi flies (so that the rhabdomeres extend the entire depth of the retina in the rescued animals). However, misshapen rhabdomeres are seen in the rescued flies and ommatidia are frequently disorganised.

Expression of Hsap\CRX^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^oc.1.6 rescues rhabdomere elongation in oc^uvi flies (so that the rhabdomeres extend the entire depth of the retina in the rescued animals). Almost all ommatidia form well-organised trapezoids.

Expression of Hsap\CRX^{K88N.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^oc.1.6 does not rescue rhabdomere or ommatidial morphology in oc^uvi flies.

Expression of either Hsap\CRX^{R90W.Scer\UAS.T:Zzzz\FLAG} or Hsap\CRX^{I138fs48.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^oc.1.6 largely rescues rhabdomere and ommatidial morphology in oc^uvi flies.

Expression of Hsap\CRX^{E80A.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^oc.1.6 partially rescues rhabdomere and ommatidial morphology in oc^uvi flies.

(Terrell et al., 2012)

Complementation and Rescue Data

Rescued by

oc^uvi is rescued by Scer\GAL4^oc.1.6/oc^UAS.Tag:FLAG

(Terrell et al., 2012)

oc^uvi is rescued by Scer\GAL4^oc.1.6/oc^UAS.FL

(McDonald et al., 2010)

oc^uvi is rescued by Scer\GAL4^oc.1.6/oc^ΔN.UAS

(McDonald et al., 2010)

Partially rescued by

oc^uvi is partially rescued by oc^ΔC.UAS/Scer\GAL4^oc.1.6

(McDonald et al., 2010)

oc^uvi is partially rescued by oc^ΔNC.UAS/Scer\GAL4^oc.1.6

(McDonald et al., 2010)

oc^uvi is partially rescued by Scer\GAL4^oc.1.6/oc^ΔA.UAS

(McDonald et al., 2010)

oc^uvi is partially rescued by Scer\GAL4^oc.1.6/oc^ΔB.UAS

(McDonald et al., 2010)

oc^uvi is partially rescued by Scer\GAL4^oc.1.6/oc^ΔAB.UAS

(McDonald et al., 2010)

oc^uvi is partially rescued by oc^ΔABC.UAS/Scer\GAL4^oc.1.6

(McDonald et al., 2010)

oc^uvi is partially rescued by Scer\GAL4^oc.1.6/oc^HD.UAS

(McDonald et al., 2010)

oc^uvi is partially rescued by oc^hs.PF

(Tahayato et al., 2003)

Comments

Expression of oc^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^oc.1.6 rescues rhabdomere elongation in oc^uvi flies (so that the rhabdomeres extend the entire depth of the retina in the rescued animals). The rescued rhabdomeres have normal morphology and ommatidial organisation is wild type.

(Terrell et al., 2012)

Expression of oc^Scer\UAS.FL or oc^ΔN.Scer\UAS under the control of Scer\GAL4^oc.1.6 rescues rhabdomere elongation and ommatidial organisation in oc^uvi flies.

Expression of either oc^ΔC.Scer\UAS, oc^{ΔNC.Scer\UAS}, oc^ΔA.Scer\UAS, oc^ΔB.Scer\UAS, oc^{ΔAB.Scer\UAS} or oc^{ΔABC.Scer\UAS} under the control of Scer\GAL4^oc.1.6 partially rescues rhabdomere elongation and ommatidial organisation in oc^uvi flies.

Expression of oc^HD.Scer\UAS under the control of Scer\GAL4^oc.1.6 weakly rescues rhabdomere elongation and ommatidial organisation in oc^uvi flies.

(McDonald et al., 2010)

Almost complete rescue of oc^uvi phenotypes is seen after five successive heat shocks before 48 hours after puparium formation (APF). The rhabdomeres appear normal in shape and are not split. Heat shocks later than 48 hours APF fail to rescue the rhabdomeric defects.

(Tahayato et al., 2003)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

oc^uvi

otd<up> uvi</up>

(Jukam et al., 2013)

otd^UVI

(Fichelson et al., 2012)

otd^uvi

(Chen and Montell, 2020, Yan et al., 2017, Mishra et al., 2016, Mahato et al., 2014, Jukam et al., 2013, Mishra et al., 2013, Terrell et al., 2012, McDonald et al., 2010, Mishra et al., 2010, Ranade et al., 2008, Sprecher et al., 2007, Tahayato et al., 2003, van den Dries et al., 1996)

otduvi

(McDonald et al., 2006)

Name Synonyms

UV-insensitive

(van den Dries et al., 1996)

Secondary FlyBase IDs

References (16)

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