FlyBase Allele Report: Dmel\Dhc64C[8-1]

General Information

Symbol

Dmel\Dhc64C^8-1

Species

D. melanogaster

Name

FlyBase ID

FBal0048198

Feature type

allele

Associated gene

Dmel\Dhc64C

Associated Insertion(s)

Carried in Construct

Also Known As

dhc^8-1

Key Links

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Gepner et al., 1996)

Mutagen

ethyl methanesulfonate

(Gepner et al., 1996)

Progenitor genotype

Cytology

Description

Genomic Southern blotting revealed no polymorphism between the mutant and wild type chromosome.

(Gepner et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Gepner et al., 1996)

Phenotype Manifest In

lipid droplet

(Gross et al., 2000)

lipid droplet | maternal effect (with Dhc64C^6-10)

(Gross et al., 2002)

Detailed Description

Statement

Reference

In embryos laid by Dhc64C^8-1/Dhc64C^6-10 females the force (applied with optical tweezers) required to stall plus end travel of lipid droplets is significantly less than for wild-type embryos. The velocity of plus end droplet travel is significantly reduced in these mutants, especially over long runs (500-1000 nm), as is the mean plus end travel distance. In addition, the frequency of pauses during plus end travel is increased. All these effects are also seen, although more weakly, in embryos from Dhc64C^8-1/+ mothers. There is no difference in the force required to stall minus end travel of lipid droplets in embryos from Dhc64C^8-1/+ mothers, although mean minus end travel distance is reduced.

(Gross et al., 2002)

Dhc64C^8-1/Dhc64C^6-10 animals survive to give fertile adults. Embryos laid by Dhc64C^8-1/Dhc64C^6-10 females develop seemingly normally through cellularisation and early gastrulation. However, lipid droplets fail to redistribute from the centre towards the periphery of these embryos during early cycle 14, in contrast to wild type. This results in embryos that are abnormally transparent from gastrulation onward.

(Gross et al., 2000)

Lethality acts during larval, pupal or pharate adult phase. Low levels (3-12%) of embryonic lethality are observed.

(Gepner et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Dhc64C[+]/Dhc64C^8-1 is an enhancer of visible | dominant phenotype of Egfr^E1

(Iyadurai et al., 2008)

Suppressor of

Statement

Reference

Dhc64C^8-1 is a suppressor of visible | dominant phenotype of DCTN1-p150^Gl-1

(Iyadurai et al., 2008)

Dhc64C^8-1/Dhc64C⁷⁷ is a suppressor of visible | dominant phenotype of DCTN1-p150^Gl-1

(Iyadurai et al., 2008)

NOT Suppressor of

Statement

Reference

Dhc64C[+]/Dhc64C^8-1 is a non-suppressor of visible | dominant phenotype of DCTN1-p150^Gl-1, S[+]/S¹

(Iyadurai et al., 2008)

Dhc64C^8-1/Dhc64C⁷⁷ is a non-suppressor of visible | dominant phenotype of DCTN1-p150^Gl-1, S[+]/S¹

(Iyadurai et al., 2008)

Phenotype Manifest In

Enhancer of

Statement

Reference

Dhc64C[+]/Dhc64C^8-1 is an enhancer of eye phenotype of Egfr^E1

(Iyadurai et al., 2008)

Suppressor of

Statement

Reference

Dhc64C^8-1 is a suppressor of eye phenotype of DCTN1-p150^Gl-1

(Iyadurai et al., 2008)

Dhc64C^8-1/Dhc64C⁷⁷ is a suppressor of eye phenotype of DCTN1-p150^Gl-1

(Iyadurai et al., 2008)

NOT Suppressor of

Statement

Reference

Dhc64C[+]/Dhc64C^8-1 is a non-suppressor of eye phenotype of DCTN1-p150^Gl-1, S[+]/S¹

(Iyadurai et al., 2008)

Dhc64C^8-1/Dhc64C⁷⁷ is a non-suppressor of eye phenotype of DCTN1-p150^Gl-1, S[+]/S¹

(Iyadurai et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

The S¹ mutation overcomes the suppression of the Gl¹ rough eye phenotype by Dhc64C^8-1, so that triple heterozygotes have an enhanced rough eye phenotype compared to Gl¹ single heterozygotes.

Gl¹ Dhc64C⁷⁷ Dhc64C^8-1 flies have completely wild-type eyes.

The enhanced rough eye phenotype of Gl¹/+ S¹/+ flies is not suppressed by Dhc64C⁷⁷/Dhc64C^8-1.

Dhc64C^8-1 dominantly enhances the eye phenotype of Egfr^E1/+ flies, resulting in a reduced, narrower eye with fewer ommatidia.

(Iyadurai et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

Dhc64C^4-19

(Gepner et al., 1996)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Dhc64C^8-1 is hemizygous lethal with Df(3L)10^H but complements all Dhc64C mutations except Dhc64C^1-1. Intragenic complementation relationships involve Dhc64C^5-6, Dhc64C^6-6, Dhc64C^6-8, Dhc64C^6-10, Dhc64C^6-12 and Dhc64C^8-1.

(Gepner et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Dhc64C^8-1

(Trovisco et al., 2016, Iyadurai et al., 2008, Delanoue and Davis, 2005)

dhc64c^8-1

(Li et al., 2023)

dhc^8-1

(Bullock et al., 2006, Bullock et al., 2006, Macdougall et al., 2003)

l(3)64Ca^8-1

Name Synonyms

Secondary FlyBase IDs

FBal0104869

References (11)

Report Sections

Open Close