FB2024_03 , released June 25, 2024
Allele: Dmel\rosev.PI
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General Information
Symbol
Dmel\rosev.PI
Species
D. melanogaster
Name
sevenless promoter construct of Kimmel
FlyBase ID
FBal0046367
Feature type
allele
Associated gene
Dmel\ro
Associated Insertion(s)
Carried in Construct
P{sevroI}
Key Links
Transgenic product class
wild_type
(Kimmel et al., 1990)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Kimmel et al., 1990)
Mutagen
in vitro construct
(Kimmel et al., 1990)
Progenitor genotype
Carried in construct
P{sevroI}
Cytology
Description

sev proximal promoter sequences from -967bp to +89bp drive expression of a 2.1kb ro fragment with a bacterial translation initiation sequence composed of both cDNA and genomic sequence which is missing the first intron, but contains the second intron and 3' untranslated region including the poly(A) site.

(Kimmel et al., 1990)
Allele components
Component
Use(s)
Regulatory region(s)
sev
Encoded product / tool
ro
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous flies have a visible eye phenotype. Many of the corneal lenses have an altered morphology, cone cells are disrupted or missing, and there are defects in primary pigment and bristle cell organisation and number. The R7 photoreceptor cell is often transformed into a R1-R6 photoreceptor type.

      (Kimmel et al., 1990)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Other
      Statement
      Reference

      rosev.PI, sevS11.Tag:MYC has viable phenotype

      (Maixner et al., 1998)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Not lethal in combination with sevS11.T:Hsap\MYC.

      (Maixner et al., 1998)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Mosaic analysis shows that ectopic expression of ro in the R7 cell alone by rosev.PI can transform it into an outer photoreceptor cell. sev14 flies carrying rosev.PI have no R7 photoreceptor cell, indicating that ro is unable to bypass the requirement for sev function to specify neuronal fate in the R7 precursor cell.

      (Kimmel et al., 1990)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      rosev.PI
      rosev.PK
      Name Synonyms
      sevenless promoter construct of Kimmel
      (Kimmel et al., 1990)
      Secondary FlyBase IDs
        References (2)