FB2024_03 , released June 25, 2024
Allele: Dmel\Hsf4
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General Information
Symbol
Dmel\Hsf4
Species
D. melanogaster
Name
FlyBase ID
FBal0044864
Feature type
allele
Associated gene
Dmel\Hsf
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Jedlicka et al., 1997, Jedlicka et al., 1996)
Progenitor genotype
Cytology
Description

Amino acid replacement: V57M. Mutation is at the C-terminal end of helix 1 in the hydrophobic core of the DNA-binding domain.

(Jedlicka et al., 1997)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:18,133,912..18,133,912 [-]
Nucleotide change:

G18133912A

Amino acid change:

V57M | Hsf-PA; V57M | Hsf-PB; V57M | Hsf-PC; V57M | Hsf-PD

(Jedlicka et al., 1997)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Hsf4 adults exhibit increased mortality upon heat induction when compared to controls.

      (Merkling et al., 2019)

      Mutant flies show a reduced rate of survival compared to wild-type controls after infection with either Enterobacter cloacae, Listeria monocytogenes or Beauveria bassiana.

      (Chen et al., 2010)

      After a pre-heat shock at 36[o]C, Hsf4 mutants show substantial synaptic thermoprotection (as measured by the percentage of boutons responding) - thermoprotection is less than in pre-heat shocked wild type controls, but is greater than in Hsf4 mutants that did not receive a pre-heat shock treatment.

      (Neal et al., 2006)

      Flies show reduced resistance to heat stress compared to control flies.

      (Nielsen et al., 2005)

      Polytene chromosome puffs are absent after a shift to the non-permissive temperature.

      (Andrulis et al., 2000)

      Homozygotes are viable at temperatures of 25oC or below. Homozygotes arrest at the 1st or 2nd larval instar stage and die after 2-3 days without any further growth or development at the non-permissive temperature of 29oC. Viability is rescued in homozygous or hemizygous Hsf2/Df(2R)PclP2 flies carrying Hsf+t8. Homozygous adults show reduced survival after a 40 minute heat treatment at temperatures of 38oC and above, when compared to wild-type flies or homozygous Hsf4 flies carrying two copies of Hsf+t8. The temperature-sensitive period is at approximately 1.5-2.5 days of development. Homozygous adults are viable and fertile when kept at 29oC upon eclosion. Females mosaic for homozygous clones in the germ line are fertile at both permissive and restrictive temperatures.

      (Jedlicka et al., 1997)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Hsf4 is rescued by Hsf+t8

      (Jedlicka et al., 1997)
      Partially rescued by

      Hsf4 is partially rescued by Hsf+t8

      (Chen et al., 2010)
      Comments

      Hsf+t8 largely rescues the increased susceptibility of Hsf4 flies to infection with Enterobacter cloacae, Listeria monocytogenes or Beauveria bassiana.

      (Chen et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Heat shock proteins are not synthesised in response to heat stress in adult homozygotes, in contrast to wild-type flies. Hsp70 synthesis is induced during recovery from anoxia, as in wild-type flies.

      (Jedlicka et al., 1997)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      References (16)