FlyBase Allele Report: Dmel\Fas2[e86]

General Information

Symbol

Dmel\Fas2^e86

Species

D. melanogaster

Name

FlyBase ID

FBal0044815

Feature type

allele

Associated gene

Dmel\Fas2

Associated Insertion(s)

Carried in Construct

Also Known As

fasII^e86

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Hebbar and Fernandes, 2005, Schuster et al., 1996, Schuster et al., 1996, Grenningloh et al., 1991)

Mutagen

P-element activity

(Grenningloh et al., 1991)

Progenitor genotype

Ecol\lacZ^Fas2-A31

(Grenningloh et al., 1991)

Cytology

Description

Imprecise excision of the P-element from Ecol\lacZ^Fas2-A31.

(Grenningloh et al., 1991)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Beumer et al., 2002, Schuster et al., 1996, Schuster et al., 1996, Grenningloh et al., 1991)

abnormal neuroanatomy | larval stage

(Davis et al., 1996)

viable

(Schuster et al., 1996, Schuster et al., 1996, Grenningloh et al., 1991)

visible | male

(Adam et al., 2003)

Phenotype Manifest In

bouton

(Beumer et al., 2002)

calyx of adult mushroom body

(Kurusu et al., 2002)

lobe system of adult mushroom body

(Kurusu et al., 2002)

male genitalia

(Adam et al., 2003)

neuromuscular junction

(Beumer et al., 2002)

synapse

(Davis et al., 1996, Schuster et al., 1996, Schuster et al., 1996)

synapse | ectopic

(Wolfgang et al., 2004)

Detailed Description

Statement

Reference

Fas2^EB112/Fas2^e86 transheterozygotes do not have mushroom body lobe defects in the adult.

(Fushima and Tsujimura, 2007)

Dorsal longitudinal muscle a (DLMa) is innervated by a single motor neuron that has, on average, 5 contact points with the muscle in both wild type and Fas2^e86 mutants.

(Hebbar and Fernandes, 2005)

Mutants exhibit a synaptic over-growth phenotype.

(Wolfgang et al., 2004)

About 5% of mutant adult males show genitalia rotation defects.

(Adam et al., 2003)

The increase in frequency seen in the synaptic drive to aCC/RP2 during the first 28hr of larval life is not affected in the hypomorphic Fas2^e86.

(Baines et al., 2002)

Mutants show overgrowth at the larval neuromuscular junction.

(Beumer et al., 2002)

In 50% of the mushroom bodies of mutant animals, gross structural defects in the lobes and calyxes are seen.

(Kurusu et al., 2002)

50% increase in number of MN boutons at muscle 6. Mild heat induced expression of CrebB-17A^a.hs during larval stages significantly increases the quantal content of the synapse without altering quantal size (as shown by measuring excitatory postsynaptic potential, EPSP). Expression of CrebB-17A^a.hs increases the efficacy of single boutons in Fas2^e86 to wild type.

(Davis et al., 1996)

In homozygous embryos the SNb fails to diverge from the ISN partially (partial bypass) in 6% segments. Fas2^e93; beat-Ia² flies have a strong beat-Ia phenotype, 86% segments have a divergence defect.

(Fambrough and Goodman, 1996)

An increase in synaptic sprouting.

(Schuster et al., 1996)

Growth of the synapse increases when Fas2 expression level is low causing a rise in number of boutons.

(Schuster et al., 1996)

Neuronal activity is normal; bursting pattern of motor neuron action potentials controlling larval locomotion is similar to wild type. Excitatory postsynaptic potentials (EPSP) reveal synaptic transmission is also normal. Number of boutons is increased compared to wild type due to reduction in Fas2 expression, but the mean quantal content or the quantal size is not altered reflecting no change in synaptic strength. The extra boutons are not silent but should have an average quantal content lower than wild type. Bouton number does not increase when Fas2^Scer\UAS.cLa is expressed from Scer\GAL4^E62-2 in Fas2^e86 larvae.

(Schuster et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Fas2^e86 has abnormal neuroanatomy phenotype, suppressible by mys^ts1

(Beumer et al., 2002)

Suppressor of

Statement

Reference

Fas2^e86 is a suppressor of abnormal neuroanatomy phenotype of mys^ts1

(Beumer et al., 2002)

Phenotype Manifest In

Suppressed by

Statement

Reference

Fas2^e86 has synapse | ectopic phenotype, suppressible by Gαs^B19

(Wolfgang et al., 2004)

Fas2^e86 has bouton phenotype, suppressible by mys^ts1

(Beumer et al., 2002)

Fas2^e86 has neuromuscular junction phenotype, suppressible by mys^ts1

(Beumer et al., 2002)

Suppressor of

Statement

Reference

Fas2^e86 is a suppressor of bouton phenotype of mys^ts1

(Beumer et al., 2002)

Fas2^e86 is a suppressor of neuromuscular junction phenotype of mys^ts1

(Beumer et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The addition of G-sα60A^B19 suppresses the synaptic over-growth phenotype seen in Fas2^e86 mutants.

(Wolfgang et al., 2004)

Bouton numbers at the larval neuromuscular junction are not significantly different from wild type in Fas2^e86 mys^ts1 double mutants.

(Beumer et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Fas2^e86 is rescued by Fas2^UAS.cLa/Scer\GAL4^E62-2

(Schuster et al., 1996)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Fas2^e86

FasII^e86

(Davis et al., 1996, Schuster et al., 1996, Schuster et al., 1996)

FasIIe⁸⁶

(Hebbar et al., 2003)

fas II^e86

(Kurusu et al., 2002)

fas2^e86

(Beumer et al., 2002)

fasII^e86

(Fushima and Tsujimura, 2007, Wolfgang et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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