Defects in the splicing of the second intron, this allows suppression by U1 snRNA, due to the weak base pairing with the 5' end of U1 RNA to the 5' splice site, to be detected. Carried in plasmid "wDR18" and co-transfected with snRNA:U1:21D and snRNA:U1:82Ea compensatory mutations into Schneider tissue culture cells.
Defects in the splicing of the second intron, this allows suppression by U1 snRNA, due to the weak base pairing with the 5' end of U1 RNA to the 5' splice site, to be detected. Carried in plasmid "wDR18" and co-transfected with snRNA:U1:21D and snRNA:U1:82Ea compensatory mutations into Schneider tissue culture cells.