FlyBase Allele Report: Dmel\pnt[05484Δ33]

General Information

Symbol

Dmel\pnt^05484Δ33

Species

D. melanogaster

Name

FlyBase ID

FBal0035451

Feature type

allele

Associated gene

Dmel\pnt

Associated Insertion(s)

Carried in Construct

Also Known As

pnt^Δ33

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(O'Neill et al., 1994)

Progenitor genotype

pnt⁰⁵⁴⁸⁴

(O'Neill et al., 1994)

Cytology

Description

Imprecise excision of P{PZ}pnt⁰⁵⁴⁸⁴ has deleted sequence from the P1 isoform specific exon '1'.

(O'Neill et al., 1994)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage | somatic clone

(Zhu et al., 2011)

decreased cell number | embryonic stage 15

(Wang et al., 2017)

decreased cell number | embryonic stage 15 (with pnt^MI03880)

(Wang et al., 2017)

lethal | recessive

(O'Neill et al., 1994)

Phenotype Manifest In

dorsal air sac primordium | somatic clone

(Cabernard and Affolter, 2005)

dorsal appendage | maternal effect | somatic clone

(Morimoto et al., 1996)

embryonic/larval oenocyte | embryonic stage 15

(Wang et al., 2017)

embryonic/larval oenocyte | embryonic stage 15 (with pnt^MI03880)

(Wang et al., 2017)

type II neuroblast | larval stage | somatic clone

(Zhu et al., 2011)

Detailed Description

Statement

Reference

Oenocytes are completely absent in pnt^05484Δ33 homozygous or pnt^05484Δ33/pnt^MI03880 transheterozygous stage 15 embryos lack oenocytes, unlike controls.

(Wang et al., 2017)

Adult midgut intestinal stem cell somatic MARCM clones homozygous for pnt^05484Δ33 do not display any growth defects during regeneration: The rate of mitosis upon infection with Pseudomonas entomophila in the mosaic midgut is comparable to controls.

(Jin et al., 2015)

pnt^05484Δ33 mutant type II neuroblast (NB) clones appear much smaller than wild type clones, with the number of both immature and mature intermediate neural progenitors reduced by almost 50%. Accordingly, the number of GMCs is also reduced by half. Cell cycle progression appears normal in pnt^05484Δ33 mutant type II NBs.

(Zhu et al., 2011)

pnt^05484Δ33 homozygous clones in the dorsal air sac primordium grow and normally but never contribute to the tip of the primordium.

(Cabernard and Affolter, 2005)

In somatic clones mutant for pnt^05484Δ33 in third instar eye discs, most R2-5 photoreceptors do not undergo G1 arrest (about 17% do), and even fewer (about 4%) differentiate.

(Yang and Baker, 2003)

Homozygous and pnt^05484Δ33/pnt^Δ88 embryos have wild-type midgut development.

(Bilder et al., 1998)

Eggs laid by mosaic females (generated using Scer\FRT/Scer\FLP1 recombination system) that have a clone of mutant follicle cells in the dorsal-anterior region or a clone that covers the entire follicle cell layer exhibit a single broad dorsal appendage 4 times wider than a wild type appendage. This is not a fusion of the two appendages but cells from the middle region taking on an appendage-producing cell fate.

(Morimoto et al., 1996)

Clones in the eye are missing photoreceptors.

(O'Neill et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

pnt^05484Δ33 is a non-enhancer of visible phenotype of BacA\p35^GMR.PH, Dp^GMR.PD, E2f1^GMR.PD

(Staehling-Hampton et al., 1999)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The growth of intestinal stem cell somatic MARCM clones expressing cic^KK100838 under the control of Scer\GAL4^tub.PU in the adult midgut is not reduced by combination with pnt^05484Δ33.

(Jin et al., 2015)

Does not enhance the phenotype of E2f^GMR.PD Dp^GMR.PD BacA\p35^GMR.PH flies.

(Staehling-Hampton et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

pnt^ksh-14

(Staehling-Hampton et al., 1999)

pnt^pjc-5

(Staehling-Hampton et al., 1999)

pnt^pjc-6

(Staehling-Hampton et al., 1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

pnt-P1

(Yang and Baker, 2003)

pnt^05484Δ33

pnt^Δ33

(Wang et al., 2017, Jin et al., 2015, Shwartz et al., 2013, Weng et al., 2012, Zhu et al., 2011, Cabernard and Affolter, 2005, Yang and Baker, 2003, Bilder et al., 1998, Morimoto et al., 1996)

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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